AB97075

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

Name: Goat Anti-Rabbit IgG (Cy3 ®) preadsorbed secondary antibody (ab97075) | Abcam
Brand: Abcam Limited
SKU: AB97075
Price: 295 USD
Availability: InStock

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(53 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 53 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB97075)

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB97075)

Image : Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

Sample : mouse endoderm cell

Preparation :

Fix in 3% PFA in PBS for 30 min at RT

Primary antibody : Rabbit anti-talin (ab71333), 1 : 100

Secondary antibody : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1 : 100

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB97075)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB97075)

Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)

Preparation :
Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 μm thickness

Primary antibody : Rabbit anti-laminin alpha 1, 1 : 400

Secondary antibody : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3®) (ab97075), 1 : 100
Nuclei were counterstained with SYTO 16

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (53)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:443 PubMed41053028

2025

A knock-in mouse model for GABRG2-related epileptic encephalopathy displays spontaneous generalized seizures and cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Dingding Shen,Jiali Wan,Xin Zhang,Jiahui Sui,Longwu Zhan,Yuqin Zheng,Yaohui Ni,Qi Zhang

Communications biology 8:1053 PubMed40665023

2025

Mechanisms and reversibility of nicotine-induced spermatogenesis impairment and DNA methylation changes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajun Cui,Chenglu Wang,Yuxuan Zheng,Yu Zhang,Sisi Luo,Zhuoran Ren,Xueyun Qin,Mo Zhang,Fang Gao,Hefeng Huang,Jing Shu,Guolian Ding

Journal of cellular and molecular medicine 29:e70643 PubMed40548841

2025

Circadian Gene NPAS2 Relieves Hypertrophic Scar Formation via CDC25A-Mediated Fibroblasts Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Wei,Yongqiang Xiao,Zhaorong Xu,Xiaodong Chen,Qiong Jiang,Yu Fu,Jianji Yan,Zhaohong Chen,Pengfei Luo,Huazhen Liu

Research (Washington, D.C.) 8:0706 PubMed40405912

2025

Defective Endothelial Glutaminolysis Contributes to Impaired Angiogenesis and Poor Ischemic Tissue Repair in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Meina Zhao,Jiaheng Zhou,Yang Hu,Xinpei Wang,Jiong An,Meijie Liu,Pengfei Zhang,Xing Zhang,Jingwen Wang,Xing Jin,Miaomiao Xi,Jia Li

Cell reports. Medicine 6:102052 PubMed40239632

2025

Catalytic neural stem cell exosomes for multi-stage targeting and synergistical therapy of retinal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqiang Yang,Xiaojun Wang,Diwei Zheng,Jing Feng,Wenjun Kong,Yue Li,Guanghui Ma,Wei Wei,Yong Tao

Nature communications 15:10747 PubMed39737919

2024

NET formation-mediated in situ protein delivery to the inflamed central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Yina Wu,Jinwon Park,Quoc-Viet Le,Junho Byun,Jaehyun Choi,Enzhen Xu,Jaiwoo Lee,Yu-Kyoung Oh

Scientific reports 14:22344 PubMed39333662

2024

High mobility group box-1 protein promotes astrocytic CCL5 production through the MAPK/NF-κB pathway following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghao Chi,Junqin Lu,Tao He,Yijia Wang,Xinli Zhou,Yuxin Zhang,Longshun Qiu

Heliyon 10:e32457 PubMed39027525

2024

LIM homeobox 1 (LHX1) induces endoplasmic reticulum stress and promotes preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Liyin Qiu,Zhaozhen Liu,Shouzhen Chen,Yiting Wu,Jianying Yan

The European journal of neuroscience 60:4503-4517 PubMed38951719

2024

Characterization of sciatic nerve myelin sheath during development in C57BL/6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Chen,Tongxin Shang,Junjie Sun,Yuhua Ji,Leilei Gong,Aihong Li,Fei Ding,Mi Shen,Qi Zhang

Journal of cellular physiology 239:e31248 PubMed38501506

2024

Naringin activates semaphorin 3A to ameliorate TGF-β-induced endothelial-to-mesenchymal transition related to atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ju Lai,Shang-Hung Chang,Ying-Chang Tung,Gwo-Jyh Chang,Celina De Almeida,Wei-Jan Chen,Yung-Hsin Yeh,Feng-Chun Tsai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB97075)

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB97075)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (53)

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