AB6941

Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

Name: Goat Anti Rabbit (IgG) secondary antibody preadsorbed Cy3.5® (ab6941) | Abcam
Brand: Abcam Limited
SKU: AB6941

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(6 Publications)

Suitable for Flow Cyt, ICC, IHC-Fr, IHC-P, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Cy3.5®

Excitation/Emission

Ex: 581nm, Em: 596nm

Applications

IHC-Fr, ICC, IHC-P, ELISA, ICC/IF, Flow Cyt

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Product details

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above

Storage buffer

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 117:105815 PubMed40541539

2025

MyD88 signalling in B cells and antibody responses during Oropouche virus-induced neurological disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Augusto Toledo-Teixeira,Pierina Lorencini Parise,Bruno Brito Pereira da Silva,Camila Lopes Simeoni,Aline Vieira,Julia Forato,Matheus Cavalheiro Martini,Mariene Ribeiro Amorim,Karina Bispo-Dos-Santos,Natália Silva Brunetti,Gabriela Fabiano de Souza,Lais Durço Coimbra,Marina Alves Fontoura,Stéfanie Primon Muraro,Priscilla Paschoal Barbosa,Valquíria Aparecida Matheus,Xinyi Hua,Pedro Manoel Mendes de Moraes Vieira,Fabiana Granja,Pritesh Lalwani,Marco Aurélio Ramirez Vinolo,Guilherme Paier Milanez,Rafael Elias Marques,Ceri Alan Fielding,William Marciel de Souza,Alessandro Dos Santos Farias,David Anthony Price,Michael Steven Diamond,Eduardo Lani Volpe Silveira,José Luiz Proenca-Modena

American journal of cancer research 13:5577-5589 PubMed38058835

2023

Detection of CSTF2 by nano fluorescent probe and its correlation with malignant biological characteristics in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongmin Jiang,Xiaozhi Liu,Yongqing Deng, Duojierenqing, Renqingcuo,Wenhan Wu,Wei Meng,Zhijiang Shao

ACS omega 6:7815-7828 PubMed33778293

2021

Power of an Organic Electron Acceptor in Modulation of Intracellular Mitochondrial Reactive Oxygen Species: Inducing JNK- and Caspase-Dependent Apoptosis of Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Saswat Mohapatra,Gaurav Das,Varsha Gupta,Prasenjit Mondal,Masashi Nitani,Yutaka Ie,Shreyam Chatterjee,Yoshio Aso,Surajit Ghosh

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:2241-2251 PubMed30332298

2018

Insulin receptor plays a central role in skin carcinogenesis by regulating cytoskeleton assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Galina Weingarten,Aya Ben Yaakov,Erez Dror,Jenny Russ,Thomas M Magin,C Ronald Kahn,Efrat Wertheimer

Journal of neuroinflammation 15:10 PubMed29316939

2018

Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface-the protective role for BDNF.

Applications

IHC-P

Species

Mouse

Albina F Ramalho,Bruna Bombassaro,Nathalia R Dragano,Carina Solon,Joseane Morari,Milena Fioravante,Roberta Barbizan,Licio A Velloso,Eliana P Araujo

Journal of neuroinflammation 14:178 PubMed28865476

2017

Inhibition of hypothalamic leukemia inhibitory factor exacerbates diet-induced obesity phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Milena Fioravante,Bruna Bombassaro,Albina F Ramalho,Nathalia R Dragano,Joseane Morari,Carina Solon,Natalia Tobar,Celso D Ramos,Licio A Velloso

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (6)

Product promise