AB6564

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

Name: Goat Anti-Rabbit IgG ( Cy5®) preadsorbed secondary antibody (ab6564) | Abcam
Brand: Abcam Limited
SKU: AB6564
Price: 235 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(104 Publications)

Goat Anti-Rabbit IgG H&L (Cy5 ®) is a preadsorbed secondary antibody that is suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 80 publications

3 Images

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

Immunofluorescence stainings of human prostate tissues

Sections were double labeled for different PAK isoforms, together with calponin (marker for smooth muscle cells), pan-cytokeratin (marker for epithelial cells of glands), or tyrosine hydroxylase (TH, marker for catecholaminergic nerves). Yellow color in merged pictures may indicate colocalization of targets. Shown are representative stainings from series with tissues from n = 5 patients for each combination.

ab6908 was used as secondary antibody to visualize various rabbit anti-PAK primary antibodies (red).

(From Figure 2 of Hennenberg et al)

Hennenberg, M. et al PLoS One. 2012;7(11):e50904. doi: 10.1371/journal.pone.0050904. Epub 2012 Nov 30 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

ab6564 was used at dilution 1/500 with the primary antibody ab24586 in ICC/IF. See the review on ab24586.

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

ab6564 was used at dilution 1/300 with the primary antibody ab19898 in Flow Cyt. See the review on ab19898.

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Cy5®

Excitation/Emission

Ex: 650nm, Em: 667nm

Applications

IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

Storage buffer

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (104)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:507 PubMed41013769

2025

E2 pretreatment alleviates aggregation of intravenously injected mesenchymal stem cells in TBI by regulating BRG1 to affect adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Wangan Li,Xiangyu Wang,Zhiming Feng,Shiting Hua,Xiaoxiong Zou,Zelin Lai,Yingqian Cai,Yuxi Zou,Yanping Tang,Cai Lin,Guobiao Luo,Xiaolin Cai,Xiaodan Jiang

International journal of molecular sciences 26: PubMed40943496

2025

ZFHX3 Knockdown Enhances Metabolic Distress in Atrial Myocytes Through Mitochondrial and Calcium Dysregulation: Mitigation by Trimetazidine.

Applications

Unspecified application

Species

Unspecified reactive species

Baigalmaa Lkhagva,Shuen-Hsin Liu,Satoshi Higa,Yu-Hsun Kao,Yi-Jen Chen

Frontiers in molecular neuroscience 18:1628860 PubMed40747242

2025

Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia.

Applications

Unspecified application

Species

Unspecified reactive species

Erick J Martinez-Colin,Ivonne Lezama,Lenin D Ochoa-de la Paz,Monica Lamas

Methods in molecular biology (Clifton, N.J.) 2944:257-277 PubMed40553289

2025

Modeling Glioma Stem Cell-Mediated Tumorigenesis Using Zebrafish Patient-Derived Xenograft Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Hema Priya Mahendran,Alan Cieslukowski,Dorota Lubanska,Nicholas Philbin,Keith F Stringer,Philip Habashy,Mat Stover,Samira Bashiri,Ana C deCarvalho,Mohamed A R Soliman,Abdalla Shamisa,Lisa A Porter

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413709 PubMed40150862

2025

UGDH Lactylation Aggravates Osteoarthritis by Suppressing Glycosaminoglycan Synthesis and Orchestrating Nucleocytoplasmic Transport to Activate MAPK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Weiren Lan,Xueman Chen,Huai Yu,Jianzhao Ruan,Jingliang Kang,Xiaoyu Nie,Yumei Cao,Su'an Tang,Changhai Ding

Nature communications 16:2300 PubMed40055357

2025

POSA for fast, amplified and multiplexed protein imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Wenkang Zhang,Hengfeng Jiang,Liang Han,Jie Liu,Jie Wang,Feng He,Leilei Tian

International journal of molecular medicine 55: PubMed40052591

2025

Inhibitors of the ubiquitin‑proteasome system rescue cellular levels and ion transport function of pathogenic pendrin (SLC26A4) protein variants.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Bernardinelli,Rapolas Jamontas,Arnoldas Matulevičius,Florian Huber,Houssein Nasser,Sophie Klaus,Haixia Zhu,Jiangang Gao,Silvia Dossena

Frontiers in cell and developmental biology 13:1450407 PubMed39974724

2025

Cisplatin resistance alters ovarian cancer spheroid formation and impacts peritoneal invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia C Powell,Marcos Quintela,David W James,Emenike Onyido,David Howard,Kadie Edwards,Jordan L Turney,Charlotte R Morgan,Jenny Worthington,Nicole Williams,Alexander Dulebo,Heiko Haschke,Deyarina Gonzalez,R Steven Conlan,Lewis W Francis

The Journal of biological chemistry 301:108177 PubMed39798872

2025

Replisomal coupling between the α-pol III core and the τ-subunit of the clamp loader complex (CLC) are essential for genomic integrity in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren J Butterworth,Malisha U Welikala,Cody W Klatt,Kaitlyn E Rheney,Michael A Trakselis

Nature communications 15:9803 PubMed39532876

2024

Complement and microglia activation mediate stress-induced synapse loss in layer 2/3 of the medial prefrontal cortex in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haven Tillmon,Breeanne M Soteros,Liang Shen,Qifei Cong,Mackenna Wollet,Julianne General,Hanna Chin,John Beichen Lee,Flavia R Carreno,David A Morilak,Jun Hee Kim,Gek Ming Sia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Alternative Products

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (104)

Product promise