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Goat Anti-Rabbit IgG H&L (Cy5 ®) is a preadsorbed secondary antibody that is suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 80 publications


Images

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564), expandable thumbnail
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
Conjugation
Cy5®
Applications
Flow Cyt, IHC-Fr, IHC-P, ICC/IF, ELISA
Clonality
Polyclonal

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Reactivity data

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/10000.00000 - 1/50000.00000
Notes

-

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Alternative names

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Goat Anti-Rabbit IgG H&L (Cy5 ®) is a preadsorbed secondary antibody that is suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 80 publications

Key facts

Description
Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
Conjugation
Cy5®
Applications
Flow Cyt, IHC-Fr, IHC-P, ICC/IF, ELISA
Clonality
Polyclonal
Pre-adsorbed
Yes
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Purification technique
Affinity purification Immunogen
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

This antibody reacts with the heavy and light chains of Rabbit IgG

Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564), expandable thumbnail
    Hennenberg, M. et al PLoS One. 2012;7(11):e50904. doi: 10.1371/journal.pone.0050904. Epub 2012 Nov 30 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)

    Immunofluorescence stainings of human prostate tissues

    Sections were double labeled for different PAK isoforms, together with calponin (marker for smooth muscle cells), pan-cytokeratin (marker for epithelial cells of glands), or tyrosine hydroxylase (TH, marker for catecholaminergic nerves). Yellow color in merged pictures may indicate colocalization of targets. Shown are representative stainings from series with tissues from n = 5 patients for each combination.

    Goat Anti-Guinea pig IgG H&L (HRP) ab6908 was used as secondary antibody to visualize various rabbit anti-PAK primary antibodies (red).

    (From Figure 2 of Hennenberg et al)

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)

    ab6564 was used at dilution 1/500 with the primary antibody ab24586 in ICC/IF. See the review on ab24586.

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)

    ab6564 was used at dilution 1/300 with the primary antibody Anti-CD133 antibody - Stem Cell Marker ab19898 in Flow Cyt. See the review on Anti-CD133 antibody - Stem Cell Marker ab19898.

Downloads

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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