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AB6564

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

5

(1 Review)

|

(82 Publications)

Goat Anti-Rabbit IgG H&L (Cy5 ®) is a preadsorbed secondary antibody that is suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 80 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images
Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)
  • IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

Immunofluorescence stainings of human prostate tissues

Sections were double labeled for different PAK isoforms, together with calponin (marker for smooth muscle cells), pan-cytokeratin (marker for epithelial cells of glands), or tyrosine hydroxylase (TH, marker for catecholaminergic nerves). Yellow color in merged pictures may indicate colocalization of targets. Shown are representative stainings from series with tissues from n = 5 patients for each combination.

ab6908 was used as secondary antibody to visualize various rabbit anti-PAK primary antibodies (red).

(From Figure 2 of Hennenberg et al)

Hennenberg, M. et al PLoS One. 2012;7(11):e50904. doi: 10.1371/journal.pone.0050904. Epub 2012 Nov 30 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

ab6564 was used at dilution 1/500 with the primary antibody ab24586 in ICC/IF. See the review on ab24586.

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)
  • Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB6564)

ab6564 was used at dilution 1/300 with the primary antibody ab19898 in Flow Cyt. See the review on ab19898.

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Cy5®

Excitation/Emission

Ex: 650nm, Em: 667nm

Applications

ICC/IF, Flow Cyt, ELISA, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"<p></p>" } } }
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You may be interested in:

AB6563

Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed

4

2 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
Storage buffer
pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (82)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed38256961

2024

Acute Kidney Injury Caused by Rhabdomyolysis Is Ameliorated by Serum Albumin-Based Supersulfide Donors through Antioxidative Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Mayumi Ikeda-Imafuku,Tatsuya Fukuta,Victor Tuan Giam Chuang,Tomohiro Sawa,Toru Maruyama,Masaki Otagiri,Tatsuhiro Ishida,Yu Ishima

Nature communications 15:438 PubMed38200007

2024

Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin Zhang

Nature communications 15:137 PubMed38167344

2024

p53 suppresses MHC class II presentation by intestinal epithelium to protect against radiation-induced gastrointestinal syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Wang,Chun-Yuan Chang,Xue Yang,Fan Zhou,Juan Liu,Jill Bargonetti,Lanjing Zhang,Ping Xie,Zhaohui Feng,Wenwei Hu

American journal of cancer research 13:5577-5589 PubMed38058835

2023

Detection of CSTF2 by nano fluorescent probe and its correlation with malignant biological characteristics in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongmin Jiang,Xiaozhi Liu,Yongqing Deng, Duojierenqing, Renqingcuo,Wenhan Wu,Wei Meng,Zhijiang Shao

International journal of molecular sciences 24: PubMed37958797

2023

NLRP3 Inflammasome Activates Endothelial-to-Mesenchymal Transition via Focal Adhesion Kinase Pathway in Bleomycin-Induced Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chih Chen,Wen-Kuang Yu,Vincent Yi-Fong Su,Han-Shui Hsu,Kuang-Yao Yang

Annals of medicine 55:2260387 PubMed37729607

2023

A 12-gene panel in estimating hormone-treatment responses of castration-resistant prostate cancer patients generated using a combined analysis of bulk and single-cell sequencing data.

Applications

Unspecified application

Species

Unspecified reactive species

Juanlan Huang,Dale Liu,Jun Li,Jing Xu,Shaowei Dong,Hao Zhang

PeerJ 11:e15894 PubMed37727693

2023

Tibial cortex transverse transport potentiates diabetic wound healing activation of SDF-1/CXCR4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shuanji Ou,Xiaodong Wu,Yang Yang,Changliang Xia,Wei Zhang,Yudun Qu,Jiaxuan Li,Bo Chen,Lilin Zhu,Changpeng Xu,Yong Qi

iScience 26:107455 PubMed37680481

2023

Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Kun Xu,Jie-Xi Liu,Chen-Xi Zheng,Lu Liu,Chao Ma,Jiong-Yi Tian,Yuan Yuan,Yuan Cao,Shu-Juan Xing,Si-Ying Liu,Qiang Li,Ya-Juan Zhao,Liang Kong,Yong-Jin Chen,Bing-Dong Sui

Cells 12: PubMed37296669

2023

TREM2 Agonism with a Monoclonal Antibody Attenuates Tau Pathology and Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Fassler,Clara Benaim,Jacob George

Journal of neuroinflammation 20:87 PubMed36997969

2023

Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxian Lun,Yubin Li,Xuefeng Gao,Zelong Gong,Xiaoliang Chen,Jinhu Zou,Chengxing Zhou,Yuanyuan Huang,Bingliang Zhou,Pengwei Huang,Hong Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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