Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB97077)

Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells.

Rab7 was detected with an Rabbit anti-Rab7 antibody, followed by ab97077 at 1/500 dilution in ICC/IF analysis of HeLa cells.

(From Figure S1 of Humphries et al)

Humphries, W.H. 4th et al PLoS One. 2011;6(10):e26626. doi: 10.1371/journal.pone.0026626. Epub 2011 Oct 24 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB97077)

Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)

Preparation :

• Fix in 3% PFA in PBS for 30 min at RT
• Incubate in 7.5% sucrose-PBS for 3h at RT
• Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
• Embed the EBs in tissue-Tek OCT compound
• Cut frozen sections to 4-20 μm thickness

Primary antibody : Rabbit anti-laminin alpha 1, 1 : 400
Secondary antibody : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1 : 100
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1 : 100
Nuclei were counterstained with SYTO 16

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (AB97077)

ab97077 (1/200) detecting ab5176 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue); ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.

Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada