Suitable for IHC-P, Flow Cyt, ICC/IF, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 35 publications.
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- The Journal of biological chemistry 299:1045992023An optimized reporter of the transcription factor hypoxia-inducible factor 1α reveals complex HIF-1α activation dynamics in single cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStevan Jeknić,Takamasa Kudo,Joanna J Song,Markus W CovertPubMed 36907438
- Scientific reports 12:186872022Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLudwig Schmiderer,David Yudovich,Leal Oburoglu,Martin Hjort,Jonas LarssonPubMed 36333335
- Scientific reports 12:148832022Brown adipose tissue dysfunction promotes heart failure via a trimethylamine N-oxide-dependent mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohko Yoshida,Ippei Shimizu,Atsuhiro Shimada,Keita Nakahara,Sachiko Yanagisawa,Minoru Kubo,Shinji Fukuda,Chiharu Ishii,Hiromitsu Yamamoto,Takamasa Ishikawa,Kuniyuki Kano,Junken Aoki,Goro Katsuumi,Masayoshi Suda,Kazuyuki Ozaki,Yutaka Yoshida,Shujiro Okuda,Shigeo Ohta,Shiki Okamoto,Yasuhiko Minokoshi,Kanako Oda,Toshikuni Sasaoka,Manabu Abe,Kenji Sakimura,Yoshiaki Kubota,Norihiko Yoshimura,Shingo Kajimura,Maria Zuriaga,Kenneth Walsh,Tomoyoshi Soga,Tohru MinaminoPubMed 36050466
- iScience 25:1045472022Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuka Hayashi,Ippei Shimizu,Yohko Yoshida,Ryutaro Ikegami,Masayoshi Suda,Goro Katsuumi,Shinya Fujiki,Kazuyuki Ozaki,Manabu Abe,Kenji Sakimura,Shujiro Okuda,Toshiya Hayano,Kazuhiro Nakamura,Kenneth Walsh,Naja Zenius Jespersen,Søren Nielsen,Camilla Scheele,Tohru MinaminoPubMed 35754738
- iScience 25:1042682022Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohko Yoshida,Ippei Shimizu,Yung-Ting Hsiao,Masayoshi Suda,Goro Katsuumi,Masahide Seki,Yutaka Suzuki,Shujiro Okuda,Tomoyoshi Soga,Tohru MinaminoPubMed 35521515
- PloS one 16:e02606452021Optimised CO2-containing medium for in vitro culture and transportation of mouse preimplantation embryos without CO2 incubator.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuyuki Kikuchi,Sayaka Wakayama,Daiyu Ito,Masatoshi Ooga,Teruhiko WakayamaPubMed 34941870
- Cancer research 82:648-6642021Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGina Bouchard,Fernando Jose Garcia-Marques,Loukia Georgiou Karacosta,Weiruo Zhang,Abel Bermudez,Nicholas McIlvain Riley,Sushama Varma,Lindsey Catherine Mehl,Jalen Anthony Benson,Joseph B Shrager,Carolyn Ruth Bertozzi,Sharon J Pitteri,Amato J Giaccia,Sylvia Katina PlevritisPubMed 34853070
- Scientific reports 11:183842021Empagliflozin maintains capillarization and improves cardiac function in a murine model of left ventricular pressure overload.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaaki Nakao,Ippei Shimizu,Goro Katsuumi,Yohko Yoshida,Masayoshi Suda,Yuka Hayashi,Ryutaro Ikegami,Yung Ting Hsiao,Shujiro Okuda,Tomoyoshi Soga,Tohru MinaminoPubMed 34526601
- iScience 24:1028152021Mailing viable mouse freeze-dried spermatozoa on postcards.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaiyu Ito,Sayaka Wakayama,Rina Emura,Masatoshi Ooga,Teruhiko WakayamaPubMed 34471856
- Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 85:161-1692021CD34 protein is expressed in murine, canine, and porcine lungs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGurpreet K Aulakh,Sushmita Maltare,Le Nguyen Phuong Khanh,Baljit SinghPubMed 34248259
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Horse, Chicken, Cow, Human, Pig
- Conjugation
- Cy5®
- Applications
- IHC-P, Flow Cyt, ICC/IF, WB
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for IHC-P, Flow Cyt, ICC/IF, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 35 publications.
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Horse, Chicken, Cow, Human, Pig
- Conjugation
- Cy5®
- Applications
- IHC-P, Flow Cyt, ICC/IF, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Humphries, W.H. 4th et al PLoS One. 2011;6(10):e26626. doi: 10.1371/journal.pone.0026626. Epub 2011 Oct 24 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab97077)
Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells.Rab7 was detected with an Rabbit anti-Rab7 antibody, followed by ab97077 at 1/500 dilution in ICC/IF analysis of HeLa cells.
(From Figure S1 of Humphries et al)
Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolImmunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab97077)
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3% PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 μm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1:100
F-actin was stained with CytoPainter F-actin staining kit (blue) (F-actin Staining Kit - Blue Fluorescence - Cytopainter ab112124), 1:100
Nuclei were counterstained with SYTO 16
Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, CanadaImmunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab97077)
ab97077 (1/200) detecting Anti-Histone H3 (phospho S10) antibody ab5176 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue); Anti-Histone H3 (phospho S10) antibody ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.
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