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Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 27 publications.


Images

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883), expandable thumbnail
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883), expandable thumbnail
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883), expandable thumbnail
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883), expandable thumbnail
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
DyLight® 488
Applications
IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Alternative names

Recommended products

Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 27 publications.

Key facts

Description
Goat Anti-Rabbit IgG H&L (DyLight® 488)
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
DyLight® 488
Applications
IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal
Pre-adsorbed
No
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed HEK293T cells permeabilized with 90% methanol labeling human Phospho KAP-1 (S824) with Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human CDK1 with Anti-CDK1 antibody [BLR085G] ab265590 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human CHD4 with Anti-CHD4 antibody [BLR066G] ab264417 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed etoposide treated HEK293T cells permeabilized with 90% methanol labeling human gamma H2AX with Anti-gamma H2A.X (phospho S139) antibody [BLR053F] ab243906 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Zeb1 with Anti-ZEB1 antibody [BLR102H] ab276129 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human MED12 with Anti-MED12 antibody [BLR084G] ab275962 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Ramos cells permeabilized with 90% methanol labeling human CD27 with Anti-CD27 antibody [BLR083G] ab265589 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human MEK2 with Anti-MEK2 antibody [BLR079G] ab265586 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Bcl11a with Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free ab264423 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Ramos cells permeabilized with 90% methanol labeling human Senataxin with Anti-Senataxin antibody [BLR050F] - BSA free ab243904 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human PBRM1 with Anti-PBRM1/BAF180 antibody [BL-39-2C3] ab243876 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling MDMX/MDM4 with Anti-MDMX/MDM4 antibody [BL-3-2F2] ab243859 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details, Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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