Goat Anti-Rabbit IgG H&L (DyLight® 488)

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human MED12 with ab275962 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Detection of human ARID2 (shaded) in Jurkat cells by flow cytometry. Antibody : Rabbit anti-ARID2 recombinant monoclonal antibody [BLR0213K] (ab314046) or isotype control (unshaded). Secondary : DyLight^® 488-conjugated goat anti-rabbit IgG (ab96883).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Immunofluorescence analysis of FFPE sections of human stomach carcinoma tissue stained for PCNA with a rabbit anti-PCNA primary used at a 1/100 dilution.

Secondary Antibody : Green-fluorescent goat anti-rabbit IgG-heavy and light chain Antibody DyLight^® 488 Conjugated ab96883 (left) and ab96883 (right) used at a 1/100 dilution (5µg/ml)

DAPI was used for nuclear staining.

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human CDK1 with ab265590 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Ramos cells permeabilized with 90% methanol labeling human CD27 with ab265589 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Zeb1 with ab276129 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Bcl11a with ab264423 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Ramos cells permeabilized with 90% methanol labeling human Senataxin with ab243904 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling MDMX/MDM4 with ab243859 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details, Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human MEK2 with ab265586 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human CHD4 with ab264417 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed etoposide treated HEK293T cells permeabilized with 90% methanol labeling human gamma H2AX with ab243906 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human PBRM1 with ab243876 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

• Flow Cyt

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Flow cytometry overlay histogram of 4% formaldehyde fixed HEK293T cells permeabilized with 90% methanol labeling human Phospho KAP-1 (S824) with ab243870 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• ELISA

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ELISA - Goat Anti-Rabbit IgG H&L (DyLight® 488) (AB96883)

Detection of human ARID2 (shaded) in Jurkat cells by flow cytometry. Antibody : Rabbit anti-ARID2 recombinant monoclonal antibody [BLR0213K] (ab314046) or isotype control (unshaded). Secondary : DyLight^® 488-conjugated goat anti-rabbit IgG (ab96883).