JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96886

Goat Anti-Rabbit IgG H&L (DyLight® 650)

5

(1 Review)

|

(10 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Cited in 10 publications.
19 Images
Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HT-29 cells permeabilized with 90% methanol labeling human SMC1 with ab243875 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Detection of human E-Cadherin (shaded) in HT29 cells by flow cytometry. Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling mouse beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed Daudi cells permeabilized with 90% methanol labeling human TFEB with ab264421 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human MED1 with ab243893 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling human beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed K562 cells permeabilized with 90% methanol labeling human CTCF with ab243897 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed HEK293T cells permeabilized with 90% methanol labeling mouse B7-H3/CD276 with ab243882 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed K562 cells permeabilized with 90% methanol labeling human BRD3 with ab264420 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Detection of human Granzyme B (shaded) in SR (human lymphoma cell line) cells by flow cytometry.

SR cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 106 cells.

Antibody : Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (ab243879) or isotype control (unshaded).

Secondary : DyLight® 650-conjugated goat anti-rabbit IgG (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed mIMCD-3 cells permeabilized with 90% methanol labeling human 53BP1 with ab243868 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details, Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed NIH3T3 cells permeabilized with 90% methanol labeling mouse B7-H3/CD276 with ab243882 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed A20 cells permeabilized with 90% methanol labeling mouse CTCF with ab243897 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed NIH3T3 cells permeabilized with 90% methanol labeling human SMC1 with ab243875 at 1 µl per 1 x 10^6 cells etoposide treated (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)
  • Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Rabbit IgG H&L (DyLight® 650) (AB96886)

Flow cytometry overlay histogram of 4% formaldehyde fixed mIMCD-3 cells permeabilized with 90% methanol labeling human MED1 with ab243893 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

ICC/IF, IHC-P, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p>DyLight<sup>®</sup> 650 replaces DyLight<sup>®</sup> 649. The DyLight<sup>®</sup> 650 excitation is 652nm and the emission is 672nm. DyLight<sup>®</sup> 650 hence provides the same far-red fluorescence and photostability as the DyLight<sup>®</sup> 649 dye. Please refer to the vial in order to check whether the product you received is DyLight<sup>®</sup> 649 or DyLight<sup>®</sup> 650. This information however will have no impact on your experiments.</p>" } } }

Product details

DyLight® 650 replaces DyLight® 649.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0316443 PubMed40029831

2025

Understanding the early molecular changes associated with radiation therapy-A preliminary bulk RNA sequencing study.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Miller,Henning De May,David L Rou,Jayant P Agarwal,Sujee Jeyapalina

Blood cancer journal 13:125 PubMed37591861

2023

The oncogenic human B-cell lymphoma MYD88 L265P mutation genocopies activation by phosphorylation at the Toll/interleukin-1 receptor (TIR) domain.

Applications

Unspecified application

Species

Unspecified reactive species

Marthe Minderman,Hildo Lantermans,Carmen van der Zwaan,Arie J Hoogendijk,Maartje van den Biggelaar,Marie José Kersten,Marcel Spaargaren,Steven T Pals

BMC molecular and cell biology 23:26 PubMed35794526

2022

Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Wei Lee,Chia-Chen Kuo,Chi-Jung Liang,Huei-Jyuan Pan,Chia-Ning Shen,Chau-Hwang Lee

British journal of pharmacology 179:1753-1768 PubMed34825370

2022

Sphingosine-1-phosphate/TGF-β axis drives epithelial mesenchymal transition in asthma-like disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Riemma,Ida Cerqua,Barbara Romano,Elena Irollo,Antonio Bertolino,Rosa Camerlingo,Elisabetta Granato,Giuseppina Rea,Stefania Scala,Michela Terlizzi,Giuseppe Spaziano,Rosalinda Sorrentino,Bruno D'Agostino,Fiorentina Roviezzo,Giuseppe Cirino

iScience 24:103018 PubMed34522861

2021

Encephalopathy-causing mutations in Gβ () alter regulation of neuronal GIRK channels.

Applications

Unspecified application

Species

Unspecified reactive species

Haritha P Reddy,Daniel Yakubovich,Tal Keren-Raifman,Galit Tabak,Vladimir A Tsemakhovich,Maria H Pedersen,Boris Shalomov,Sophie Colombo,David B Goldstein,Jonathan A Javitch,Amal K Bera,Nathan Dascal

International journal of molecular sciences 20: PubMed31717998

2019

Mechanisms of Neuronal Death in the Cerebral Cortex during Aging and Development of Alzheimer's Disease-Like Pathology in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Darya V Telegina,Gleb K Suvorov,Oyuna S Kozhevnikova,Nataliya G Kolosova

Scientific reports 9:6749 PubMed31043642

2019

G protein-coupled estrogen receptor mediates anti-inflammatory action in Crohn's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Damian Jacenik,Marta Zielińska,Anna Mokrowiecka,Sylwia Michlewska,Ewa Małecka-Panas,Radzisław Kordek,Jakub Fichna,Wanda M Krajewska

Scientific reports 9:508 PubMed30679535

2019

Mutual action by Gγ and Gβ for optimal activation of GIRK channels in a channel subunit-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Galit Tabak,Tal Keren-Raifman,Uri Kahanovitch,Nathan Dascal

Aging 8:2713-2733 PubMed27750209

2016

An antioxidant specifically targeting mitochondria delays progression of Alzheimer's disease-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia A Stefanova,Natalia A Muraleva,Kseniya Yi Maksimova,Ekaterina A Rudnitskaya,Elena Kiseleva,Darya V Telegina,Nataliya G Kolosova

Journal of pineal research 59:163-77 PubMed25988948

2015

Melatonin attenuates impairments of structural hippocampal neuroplasticity in OXYS rats during active progression of Alzheimer's disease-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia A Stefanova,Kseniya Y Maksimova,Elena Kiseleva,Ekaterina A Rudnitskaya,Natalia A Muraleva,Nataliya G Kolosova
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com