JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96902

Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

Be the first to review this product! Submit a review

|

(10 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p>DyLight<sup>®</sup> 650 replaces DyLight<sup>®</sup> 649. The DyLight<sup>®</sup> 650 excitation is 652nm and the emission is 672nm. DyLight<sup>®</sup> 650 hence provides the same far-red fluorescence and photostability as the DyLight<sup>®</sup> 649 dye. Please refer to the vial in order to check whether the product you received is DyLight<sup>®</sup> 649 or DyLight<sup>®</sup> 650. This information however will have no impact on your experiments.</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

DyLight® 650 replaces DyLight® 649.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Rabbit IgG
style
left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:874756 PubMed35923848

2022

Preliminary Study of S100B and Sema3A Expression Patterns in Regenerating Muscle Implicates P75-Expressing Terminal Schwann Cells and Muscle Satellite Cells in Neuromuscular Junction Restoration.

Applications

Unspecified application

Species

Unspecified reactive species

Nasibeh Daneshvar,Judy E Anderson

Virulence 12:2214-2227 PubMed34494942

2021

The ApoA-I mimetic peptide 4F attenuates in vitro replication of SARS-CoV-2, associated apoptosis, oxidative stress and inflammation in epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Theodoros Kelesidis,Sharma Madhav,Anton Petcherski,Hugo Cristelle,Ellen O'Connor,Nan W Hultgren,Eleni Ritou,David S Williams,Orian S Shirihai,Srinivasa T Reddy

Anticancer research 41:27-42 PubMed33419797

2021

Molecular Targeting of H/MDM-2 Oncoprotein in Human Colon Cancer Cells and Stem-like Colonic Epithelial-derived Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anusha Thadi,William F Morano,Marian Khalili,Blake D Babcock,Mohammad F Shaikh,Deshka S Foster,Yelena Piazza,Elizabeth M Gleeson,Eve Goldstein,Lindsay Steele,Paul M Campbell,B O Lin,Matthew R Pincus,Wilbur B Bowne

American journal of physiology. Cell physiology 319:C116-C128 PubMed32374678

2020

Premature satellite cell activation before injury accelerates myogenesis and disrupts neuromuscular junction maturation in regenerating muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Nasibeh Daneshvar,Ryuichi Tatsumi,Jason Peeler,Judy E Anderson

Molecular therapy : the journal of the American Society of Gene Therapy 26:2580-2591 PubMed30143435

2018

Transvascular Delivery of Hydrophobically Modified siRNAs: Gene Silencing in the Rat Brain upon Disruption of the Blood-Brain Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno M D C Godinho,Nils Henninger,James Bouley,Julia F Alterman,Reka A Haraszti,James W Gilbert,Ellen Sapp,Andrew H Coles,Annabelle Biscans,Mehran Nikan,Dimas Echeverria,Marian DiFiglia,Neil Aronin,Anastasia Khvorova

Artificial organs 42:736-745 PubMed29660790

2018

Development of a Tissue-Engineered Artificial Ligament: Reconstruction of Injured Rabbit Medial Collateral Ligament With Elastin-Collagen and Ligament Cell Composite Artificial Ligament.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Hirukawa,Shingo Katayama,Tatsuya Sato,Kota Inoue,Kosuke Niwa,Naoya Ito,Tetsuya Hattori,Takashi Hosoi,Hironori Unno,Yoshiaki Suzuki,Masahiro Hasegawa,Keiichi Miyamoto,Takashi Horiuchi

Cell stress & chaperones 22:589-600 PubMed28337642

2017

The small heat shock proteins αB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of α-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Dezerae Cox,Heath Ecroyd

Scientific reports 6:32939 PubMed27609527

2016

Roles and regulation of autophagy and apoptosis in the remodelling of the lepidopteran midgut epithelium during metamorphosis.

Applications

IF

Species

Unspecified reactive species

Davide Romanelli,Morena Casartelli,Silvia Cappellozza,Magda de Eguileor,Gianluca Tettamanti

The Journal of biological chemistry 288:2167-78 PubMed23233672

2012

A shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins.

Applications

ICC/IF

Species

Unspecified reactive species

Marina Shenkman,Bella Groisman,Efrat Ron,Edward Avezov,Linda M Hendershot,Gerardo Z Lederkremer

Molecular biology of the cell 22:3715-24 PubMed21832153

2011

Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors.

Applications

ICC/IF

Species

Unspecified reactive species

Rashid Mehmood,Noriko Yasuhara,Masahiro Fukumoto,Souichi Oe,Taro Tachibana,Yoshihiro Yoneda
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Secondary Antibodies

AB6717

Goat Anti-Rabbit IgG H&L (FITC)

secondary-antibodies

goat-rabbit-igg-h-l-fitc-ab6717

5

(2 reviews)

Alternative Product
Secondary Antibodies

AB175471

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-568-ab175471

5

(1 reviews)

Alternative Product
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-488-ab150077

5

(20 reviews)

Alternative Product
Secondary Antibodies

AB150079

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-647-ab150079

3

(1 reviews)

Alternative Product
Secondary Antibodies

AB150080

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-594-ab150080

5

(3 reviews)

Alternative Product
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

secondary-antibodies

goat-rabbit-igg-h-l-hrp-ab205718

5

(8 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com