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AB6717

Goat Anti-Rabbit IgG H&L (FITC)

5

(2 Reviews)

|

(585 Publications)

Goat anti-rabbit IgG H&L (FITC) (ab6717) is a secondary antibody with a maximum absorption wavelength of 495 nm and a maximum emission wavelength of 519 nm. Suitable for ICC/IF, IHC, Flow cytometry and ELISA applications.

- Ideal for multi-color fluorescent cell and tissue imaging and analysis.
- Proven performance: cited in over 580 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (AB6717)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (AB6717)

Subcellular localisation of PDCD4 was assessed by immunocytochemical staining. ARIP cells were seeded at 3×105 cells/well on sterile cover slips in six well plates and incubated overnight under standard culture conditions.

Following specific experimental conditions, media was removed and cells were washed with PBS and fixed with 3.7% formalin. Cells were then permeabilised with 0.1% triton X-100 in PBS followed by blocking in blocking buffer (10% Goat serum; 2% BSA; 0.2% Tween 20; 0.7% Glycerol in PBS) for 1 hour. Cells were incubated overnight with ab51495 at 1/100 dilution followed by ab6717 (green) at 1/80 dilution. The nuclear counterstain is DAPI (Blue).

Results are representative of three separate experiments and images were representative of six separate fields. PDCD4 localized and expressing At G0 : Cytoplasmic and very low expression; At N12 : Cytoplasmic and low expression; At N24 : very high cytoplasmic and low nuclear expression; At H12 : Cytoplasmic expression and At H24 very high cytoplasmic and low nuclear expression. Over-all PDCD4 was highly expressed in the cytoplasm, with very low expression under serum starved conditions

Kumar S et al. The role of tumour suppressor PDCD4 in beta cell death in hypoxia. PLoS One 12:e0181235 (2017). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (AB6717)
  • Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (AB6717)

ab6717 was used with the primary antibody ab66506 in Flow Cyt. See Abreview on ab66506.

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-FoFr, IHC-Fr, IM, ELISA, ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-FoFr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" } } }
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AB6785

Goat Anti-Mouse IgG H&L (FITC)

5

1 Reviews

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (585)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e28639 PubMed38586324

2024

Melatonin increases AKT and SOD gene and protein expressions in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Lotfy,Aalaa Khattab,Mohammed Shata,Ahmad Alhasbani,Abdallah Khalaf,Saeed Alsaeedi,Mahdi Thaker,Hazza Said,Harun Tumi,Hassan Alzahmi,Omar Alblooshi,Mohamad Hamdan,Amjad Hussein,Biduth Kundu,Ernest A Adeghate

British journal of cancer 130:1599-1608 PubMed38519706

2024

Persistence of peripheral CD8 + CD28- T cells indicates a favourable outcome and tumour immunity in first-line HER2-positive metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoran Liu,Xiangming Cheng,Feng Xie,Kun Li,Yongcan Shi,Bin Shao,Xu Liang,Fengling Wan,Shidong Jia,Yue Zhang,Yiqiang Liu,Huiping Li

Cell biology international 48:872-882 PubMed38480956

2024

Curcumin relieves oxaliplatin-induced neuropathic pain via reducing inflammation and activating antioxidant response.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Wei Zhang,Xu Sun,Yi-Wen Xu,Wei Meng,Qiong Tang,Hui Gao,Ling Liu,Shao-Hui Chen

Communications biology 7:67 PubMed38195842

2024

SH3RF2 contributes to cisplatin resistance in ovarian cancer cells by promoting RBPMS degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Ting Gong,Fang-Hua Liu,Qian Xiao,Yi-Zi Li,Yi-Fan Wei,He-Li Xu,Fan Cao,Ming-Li Sun,Feng-Li Jiang,Tao Tao,Qi-Peng Ma,Xue Qin,Yang Song,Song Gao,Lang Wu,Yu-Hong Zhao,Dong-Hui Huang,Qi-Jun Wu

PloS one 19:e0294514 PubMed38165884

2024

Cathepsin B aggravates atherosclerosis in ApoE-deficient mice by modulating vascular smooth muscle cell pyroptosis through NF-κB / NLRP3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Quanwei Zhao,Danan Liu,Bo Zhou,Fujun Liao,Long Chen

Experimental and therapeutic medicine 27:44 PubMed38144917

2023

Emodin inhibits HDAC6 mediated NLRP3 signaling and relieves chronic inflammatory pain in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ding-Wen Cheng,Yiwen Xu,Tao Chen,Shu-Qing Zhen,Wei Meng,Hai-Li Zhu,Ling Liu,Min Xie,Fangshou Zhen

Frontiers in immunology 14:1307693 PubMed38143750

2023

Novel S2 subunit-specific antibody with broad neutralizing activity against SARS-CoV-2 variants of concern.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Kyu Heo,Won-Hee Lim,Jihyun Yang,Sumin Son,Sang Jick Kim,Doo-Jin Kim,Haryoung Poo,Eun-Wie Cho

Toxics 11: PubMed38133381

2023

Early-Life Exposure to the Mycotoxin Fumonisin B1 and Developmental Programming of the Ovary of the Offspring: The Possible Role of Autophagy in Fertility Recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Awadh Alhelaisi,Abdulkarem Alrezaki,Saber Nahdi,Waleed Aldahmash,Saleh Alwasel,Abdel Halim Harrath

Toxins 15: PubMed37999527

2023

Involvement of Autophagy and Oxidative Stress-Mediated DNA Hypomethylation in Transgenerational Nephrotoxicity Induced in Rats by the Mycotoxin Fumonisin B1.

Applications

Unspecified application

Species

Unspecified reactive species

Nouf Aldawood,Sarah Almustafa,Saleh Alwasel,Waleed Aldahmash,Abir Ben Bacha,Abdullah Alamri,Mohammad Alanazi,Abdel Halim Harrath

American journal of cancer research 13:5577-5589 PubMed38058835

2023

Detection of CSTF2 by nano fluorescent probe and its correlation with malignant biological characteristics in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongmin Jiang,Xiaozhi Liu,Yongqing Deng, Duojierenqing, Renqingcuo,Wenhan Wu,Wei Meng,Zhijiang Shao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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