Goat Anti-Rabbit IgG H&L (FITC)

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (AB97050)

Immunocytochemical/immunofluorescent analysis of 4% paraformaldehyde-fixed Triton X-100 permeabilized Goat skeletal muscle-derived satellite cells stained for NSE using ab53025.

After washing in PBS, the cells were incubated with Goat Anti-Rabbit IgG H&L (FITC) (ab97050) (Green).

Ren Y et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014).

• Flow Cyt

AbReview36849****

Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (AB97050)

ab84235 staining melanoma inhibitory activity in a human melanoma cell line by Flow Cytometry. Cells were harvested with EDTA and washed in PBS. Cells were pemeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. A FITC-conjugated goat anti-rabbit IgG H&L (ab97050) (1/100) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (AB97050)

The FACS staining was performed on HEK cells expressing human CCR5 with PBS/BSA (0.5%)/Azide (0.05%) as FACS buffer. We used a primary polyclonal rabbit antibody against CCR5 at a 1/100 dillution, incubated for 1 hour at 4C. The secondary antibody was ab97050 used at a concentration of 1/100 for 30min at 4C. No fixation or permeabilization was performed and gaiting was done on alive cells.

This image is courtesy of an anonymous Abreview

• Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (AB97050)

The FACS staining was perform on THP-1 cell lines with Rabbit polyclonal to CCR2 (ab21667) at a 1/100 dillution for 30 min at 4C. The secondary antibody was ab97050 used at 1/100 for 20min at 4C. The buffer used was PBS/BSA (0.5%)/Azide (0.05%). No fixation or permeabilization was performed and gating was done on alive cells.

This image is courtesy of an anonymous Abreview

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (FITC) (AB97050)

Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)

Preparation :

• Fix in 3% PFA in PBS for 30 min at RT
• Incubate in 7.5% sucrose-PBS for 3h at RT
• Incubate in 15% sucrose-PBS at 4° overnight
• Embed the EBs in tissue-Tek OCT compound
• Cut frozen sections to 4-20 μm thickness

Primary antibody : Rabbit anti-laminin alpha 1, 1 : 400
Secondary antibody : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1 : 1000
Nuclei were counterstained stained with DRAQ7™ (ab109202), 1 : 1000

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School