Suitable for ICC, IHC-Fr, IHC-P, WB, ELISA.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info 1/10.00000 - 1/100.00000 | Notes Substrate:15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness. |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/400 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/100.00000 - 1/1000.00000 | Notes Substrate:10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm. |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Suitable for ICC, IHC-Fr, IHC-P, WB, ELISA.
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
ab136782 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
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