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Suitable for Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB. Ideal for western blot. Cited in 3099 publications.

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB

Clonality

Polyclonal

Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/120000
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
1/1000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/1000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/2000 - 1/20000
Notes

Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.

Images

Target data

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Suitable for Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB. Ideal for western blot. Cited in 3099 publications.

Key facts

Description

Goat Anti-Rabbit IgG H&L (HRP)

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB

Clonality

Polyclonal

Pre-adsorbed

No

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate

Purification technique

Affinity purification Immunogen

Purification notes

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer's feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.

Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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