Goat Anti-Rabbit IgG H&L (HRP)

36 product images

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Lysates:
  Lane 1,3,5: 0.5µg/lane untreated histone
  Lane 2,4,6: 0.5µg/lane colcemid treated histone - treated
  Primary antibody:
  Lane 1-2 : Anti-Histone H3 (phospho S10) antibody ab5176 (1g/ml)
  Lane 3-4 :Anti-Histone H3 (phospho S10) antibody ab5176 (1g/ml) + phospho peptide (Anti-Histone H3 (phospho S10) antibody ab5176) 1g/ml
  Lane 5-6 :Anti-Histone H3 (phospho S10) antibody ab5176 (1g/ml) + non-phospho peptide ( Human Histone H3 (unmodified ) peptide ab2903 batch 3) 1g/ml

  All lanes: Western blot - Anti-Histone H3 (phospho S10) antibody (Anti-Histone H3 (phospho S10) antibody ab5176) at 1 µg/mL

  Lanes 1, 3 and 5: untreated histones at 0.5 µg

  Lanes 2, 4 and 6: colcemid treated histones at 0.5 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 15 kDa

  Observed band size: 15-20 kDa

  Exposure time: 10s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Rabbit polyclonal to RAD54 (Anti-RAD54 antibody ab10705) at 1/500.

  Lane 1 Marker
  Lane 2 HeLa nuclear extract
  Lane 3 HeLa cell lysate
  Lane 4 A432 cell lysate
  Lane 5 MCF7 cell lysate
  Lane 6 HEK 293 cell lysate

  Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.

  Lane 1: Western blot - Anti-RAD54 antibody (Anti-RAD54 antibody ab10705)

  Lanes 2 - 6: Western blot - Anti-RAD54 antibody (Anti-RAD54 antibody ab10705) at 1/500 dilution

  Lane 1: Marker

  Lane 2: HeLa nuclear cell lysate

  Lane 3: HeLa cell lysate

  Lane 4: A432 cell lysate

  Lane 5: MCF7 cell lysate

  Lane 6: Hek 293 cell lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Predicted band size: 84 kDa

  Observed band size: 70 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Lane 1: Western blot - Anti-RBBP4 antibody (Anti-RBBP4 antibody ab1765) at 1/500 dilution

  Lane 2: Western blot - Anti-RBBP4 antibody (Anti-RBBP4 antibody ab1765) at 1/1000 dilution

  All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

  Predicted band size: 48 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (Anti-Histone H2A.Z antibody - ChIP Grade ab4174) at 1/500 dilution

  All lanes: Calf thymus histone lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 14 kDa

  Observed band size: 15 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (Anti-beta Tubulin antibody - Loading Control ab6046) at 1/500 dilution

  Lanes 1 and 5: HeLa Cell lysate at 20 µg

  Lanes 2 and 6: A431 Cell lysate at 20 µg

  Lanes 3 and 7: MCF7 Cell lysate at 20 µg

  Lanes 4 and 8: 293 Cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 49 kDa

  Observed band size: 55 kDa

  Exposure time: 10s

• 

  Farah et al PLoS One. 2018 Feb 2;13(2):e0191526. doi: 10.1371/journal.pone.0191526. eCollection 2018. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Anti-phospho-tau immunostaining in Dryocopus lineatus.
  

  Tau-positivity in the midbrain (Panel A (shown) and B) and the corpus callosum (C) of the Dryocopus lineatus brain. The axonal tract staining demonstrates a thread-like pattern, similar to that seen with Gallyas sliver staining. Occasional intracellular tau-accumulations were identified within neurons (D).

  For full method, please see paper.

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-beta Actin antibody (Anti-beta Actin antibody ab8227) at 1/1000 dilution

  Lanes 1 and 6: HeLa nuclear lysate at 20 µg

  Lanes 2 and 7: HeLa whole cell lysate at 20 µg

  Lanes 3 and 8: A431 cell lysate at 20 µg

  Lane 4: Jurkat cell lysate at 20 µg

  Lanes 10 and 5: HEK 293 cell lysate at 20 µg

  Lane 9: Jurkate cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 41 kDa

  Observed band size: 41.7 kDa

  Exposure time: 5s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade ab8895 is specific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. This is shown in lane 2 where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (Human Histone H3 (mono methyl K4) peptide ab1340).

  All lanes: Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade ab8895) at 1/500 dilution

  All lanes: Calf thymus histone lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 15 kDa

  Observed band size: 18 kDa

  Exposure time: 2min

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade ab9053 is specifically blocked by the addition of the immunizing peptide (Human Histone H4 (tri methyl K20) peptide ab17567).

  All lanes: Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade ab9053) at 1 µg/mL

  All lanes: Calf thymus histone lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 11 kDa

  Observed band size: 13 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Rabbit polyclonal to Histone H3 (phospho T11) - Anti-Histone H3 (phospho T11) antibody ab5168 at 1/500 dilution.

  Lane 1 contains 1ug calf thymus histone prep.

  Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)

  All lanes: Western blot - Anti-Histone H3 (phospho T11) antibody (Anti-Histone H3 (phospho T11) antibody ab5168) at 1/500 dilution

  All lanes: Calf thymus histone lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

  Performed under reducing conditions.

  Predicted band size: 15 kDa

  Observed band size: 17 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade ab1791 overnight at 4°C.
  

  Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody was used for detection.

  Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade ab1791) at 1/1000 dilution

  Lanes 1 and 4: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lanes 2 and 5: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

  Lanes 3 and 6: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 15 kDa

  Observed band size: 17 kDa

  Exposure time: 10s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-Histone H4 antibody - ChIP Grade (Anti-Histone H4 antibody - ChIP Grade ab10158) at 1 µg/mL

  Lanes 1 and 3: Histone prep at 20 µg

  Lane 2: HeLa histone lysate at 20 µg

  Lane 4: HeLa Histone lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Secondary ab: Alexa Fluor 680 Goat anti-rabbit IgG
  
  0.5μg histone prep used per lane

  Primary antibody:
  Lane 1: Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048 (Histone H3 Mono Methyl K36) 1/500
  Lane 2: Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1783 (Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048) (Histone H3 Mono Methyl K36) peptide 1μg/ml
  Lane 3: Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048 (Histone H3 Mono Methyl K36) 1/500 + ab1794 (Anti-Histone H3 (di methyl K36) antibody - ChIP Grade ab9049) (Histone H3 Di Methyl K36) peptide 1μg/ml
  Lane 4: Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048 (Histone H3 Mono Methyl K36) 1/500 + Human Histone H3 (tri methyl K36) peptide ab1785 (Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade ab9050) (Histone H3 Tri Methyl K36) peptide 1μg/ml
  Lane 5: Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048 (Histone H3 Mono Methyl K36) 1/500 + Human Histone H3 (unmodified ) peptide ab2623 (Histone H3 (23-34) – unmodified) peptide 1μg/ml
  Lane 6: Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048 (Histone H3 Mono Methyl K36) 1/500 + Human Histone H3 (mono methyl K4) peptide ab1340 (Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade ab8895) (Histone H3 Mono methyl K4) peptide 1μg/ml

  Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048 specifically recognise

  All lanes: Western blot - Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade ab9048) at 1/500 dilution

  All lanes: Histone prep at 0.5 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 15 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial Loading Control (Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial Loading Control ab15895) at 1 µg/mL

  Lanes 1 and 6: Hela Nuclear lysate at 20 µg

  Lanes 2 and 7: Hela cell lysate at 20 µg

  Lanes 3 and 8: A431 cell lysate at 20 µg

  Lanes 4 and 9: Jurkat cell lysate at 20 µg

  Lanes 10 and 5: HEK293 cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 31 kDa

  Observed band size: 31 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Lanes 1 & 3: Sumo 2+3 antibody (Anti-Sumo 2 + Sumo 3 antibody ab3742) at 1/500 dilution
  Lanes 2 & 4: Sumo 2+3 antibody (Anti-Sumo 2 + Sumo 3 antibody ab3742) at 1/1000 dilution
  Lanes 1-4: HeLa nuclear extract at 20 ug

  Lane 1: as above
  Lane 2: as above
  Lane 3: Sumo 2+3 peptide (ab13760) at 1 ug/ml
  Lane 4: Sumo 2+3 peptide (ab13760) at 1 ug/ml

  
  Secondary
  Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time: 1 minute

  Predicted band sizes : 11.6 & 10.

  Lanes 1 and 3: Western blot - Anti-Sumo 2 + Sumo 3 antibody (Anti-Sumo 2 + Sumo 3 antibody ab3742) at 1/500 dilution

  Lanes 2 and 4: Western blot - Anti-Sumo 2 + Sumo 3 antibody (Anti-Sumo 2 + Sumo 3 antibody ab3742) at 1/1000 dilution

  All lanes: HeLa nuclear extract at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 12 kDa

  Observed band size: 15 kDa, 18 kDa

  Exposure time: 1min

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Western blot, on recombinant Human CTGF produced in E. coli, using Anti-CTGF antibody ab5097 at 1/500.
  
  Not yet tested against endogenous CTGF
  

  Secondary antibody - anti-rabbit HRP (ab6721)

  All lanes: Western blot - Anti-CTGF antibody (Anti-CTGF antibody ab5097) at 1/500 dilution

  Lanes 1 - 2: recombinant human CTGF at 0.01 µg

  Lanes 3 - 4: recombinant human CTGF at 0.1 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 38 kDa

  Observed band size: 38 kDa

  Exposure time: 10s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade ab9053 is specifically blocked by the addition of the immunizing peptide (Human Histone H4 (tri methyl K20) peptide ab17567).

  All lanes: Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade ab9053) at 1 µg/mL

  All lanes: Calf thymus histone lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 11 kDa

  Observed band size: 13 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Rabbit polyclonal to BRG1 (Anti-BRG1 antibody ab4081) at 1/500 on HeLa Nuclear extract (20 ug per lane).

  Lane 1: Anti-BRG1 antibody ab4081 (1/500)
  Lane 2: Anti-BRG1 antibody ab4081 (1/500) + blocking peptide (1 ug)

  Secondary antibody: Goat anti-rabbit HRP - ab6721

  All lanes: Western blot - Anti-BRG1 antibody (Anti-BRG1 antibody ab4081) at 1/500 dilution

  All lanes: HeLa Nuclear extract at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Predicted band size: 185 kDa

  Observed band size: 235 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-Cyclophilin B antibody (Anti-Cyclophilin B antibody ab16045) at 1 µg/mL

  Lane 1: Rat Liver at 20 µg

  Lane 2: Mouse 3T3 at 20 µg

  Lane 3: Dog at 20 µg

  Lane 4: Chicken at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 24 kDa

  Observed band size: 25 kDa

  Exposure time: 30s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-Cyclophilin B antibody (Anti-Cyclophilin B antibody ab16045) at 1 µg/mL

  Lanes 1 and 6: HeLa nuclear lysate at 20 µg

  Lanes 2 and 7: HeLa whole cell lysate at 20 µg

  Lanes 3 and 8: A431 whole cell lysate at 20 µg

  Lanes 4 and 9: Jurkat whole cell lysate at 20 µg

  Lanes 10 and 5: HEK293 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 24 kDa

  Observed band size: 21 kDa

  Exposure time: 30s

• 

  This image is courtesy of a customer review submitted by Maryna Polyakova.

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Blocked with 5% non-fat milk for 1 hour at 18°C

  All lanes: Anti-NEK7 antibody (ab80948) at 1/2000 dilution

  All lanes: Mouse brain tissue cytoplasmic lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Exposure time: 10s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-RUNX2 antibody (Anti-RUNX2 antibody ab23981) at 1/1000 dilution

  Lane 1: Bone tissue (tibia) from 17 weeks old Wistar male rats treated with doxorubicin hydrochloride injections for 7 weeks.

  Lane 2: Bone tissue (tibia) from 20 weeks old Wistar female rats.

  Lane 3: Bone tissue (tibia) from 56 weeks old ooforectomized Wistar female rats.

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

  Performed under reducing conditions.

  Predicted band size: 57 kDa

  Observed band size: 57 kDa

  Exposure time: 40s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Lanes 1, 3 and 4: Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody ab5131) at 1/500 dilution

  Lane 2: Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody ab5131) at 1/2000 dilution

  All lanes: HeLa nuclear extract at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 217 kDa

  Observed band size: 250 kDa

  Exposure time: 30s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Lane 1 - 2 : EEA1 antibody - Early Endosome Marker (Anti-EEA1 antibody - Early Endosome Marker ab2900) at 1/500 dilution, HEK293 Whole Cell lysate at 20 ug
  Lane 1 : as above
  Lane 2 : EEA1 peptide (ab14946) at 1 ug
  
  Secondary
  Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 160kD
  Observed band size : 180kD (why is the actual band size different from the predicted?)

  All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (Anti-EEA1 antibody - Early Endosome Marker ab2900) at 1/500 dilution

  All lanes: HEK293 Whole Cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 162 kDa

  Observed band size: 180 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (Anti-Histone H3 (acetyl K18) antibody - ChIP Grade ab1191) at 1 µg/mL

  All lanes: Histone prep at 0.5 µg/mL

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 15 kDa

  Observed band size: 17 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  The Western blot shows that ab14944 reacts strongly with mouse 3T3 and CHO cell lysates. Weak cross-reactivity is seen with Xenopus, mouse brain, mouse liver, rat brain and dog lysates. The antibody does not appear to cross-react with rat liver lysate.

  All lanes: Western blot - Anti-EEA1 antibody - Early Endosome Marker (Anti-EEA1 antibody - Early Endosome Marker ab2900) at 1 µg/mL

  Lane 1: Xenopus laevis lysate at 20 µg

  Lane 2: Mouse 3T3 cell lysate at 20 µg

  Lane 3: Mouse brain cell lysate at 20 µg

  Lane 4: Mouse liver cell lysate at 20 µg

  Lane 5: Rat brain cell lysate at 20 µg

  Lane 6: Rat liver cell lysate at 20 µg

  Lane 7: Dog lysate at 20 µg

  Lane 8: CHO cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 162 kDa

  Observed band size: 100 kDa, 180 kDa

  Exposure time: 30s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-beta Actin antibody (Anti-beta Actin antibody ab8227) at 1/1000 dilution

  Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 20 µg

  Lanes 2 and 7: HeLa whole cell lysate at 20 µg

  Lane 3: A431 (human epidermoid carcinoma cell line) cell lysate at 20 µg

  Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 20 µg

  Lane 5: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg

  Lane 6: HeLa nuclear lysate at 20 µg

  Lane 8: A431 cell lysate at 20 µg

  Lane 9: Jurkat cell lysate at 20 µg

  Lane 10: HEK-293 cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 41 kDa

  Observed band size: 41.7 kDa

  Exposure time: 5s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-beta Actin antibody (Anti-beta Actin antibody ab8227) at 1/1000 dilution

  Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cells at 20 µg

  Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) cells at 20 µg

  Lane 3: Fish Liver at 20 µg

  Lane 4: Rabbit Liver at 20 µg

  Lane 5: MDCK (Canine kidney cell line) cells at 20 µg

  Lane 6: EBTr (cow trachea) cells at 20 µg

  Lane 7: SL-29 (chicken day 11 embryo) cells at 20 µg

  Lane 8: CHO (Chinese hamster ovary cell line) cells at 20 µg

  Lane 9: Xenopus laevis embryo at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 41 kDa

  Observed band size: 41.7 kDa

  Exposure time: 30s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-gamma H2A.X (phospho S139) antibody (Anti-gamma H2A.X (phospho S139) antibody ab2893) at 1/500 dilution

  Lanes 1, 3 and 5: Control HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate Histone preparation

  Lanes 2, 4 and 6: Colcemid treated HeLa whole cell lysate Histone preparation

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 15 kDa

  Observed band size: 17 kDa, 50 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Anti-alpha Tubulin antibody - Loading Control ab4074 cross-reacts strongly with Mouse 3T3 lysate and shows weak cross-reactivity with Rat liver, Cow, Chicken and CHO lysates.

  All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody - Loading Control ab4074) at 1 µg/mL

  Lane 1: Mouse 3T3 lysate at 20 µg

  Lane 2: Rat liver lysate at 20 µg

  Lane 3: Cow cell lysate at 20 µg

  Lane 4: Chicken cell lysate at 20 µg

  Lane 5: CHO lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 50 kDa

  Observed band size: 50 kDa

  Exposure time: 30s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Primary antibody incubated for 15 hours at 4°C in TBST.
  

  3% BSA used as blocking agent for 1 hour at 23°C.

  All lanes: Western blot - Anti-TGF beta Receptor I antibody (Anti-TGF beta Receptor I antibody ab31013) at 1/1000 dilution

  Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

  Lane 2: A2780 (Human ovarian cancer cell line) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 55 kDa

  Exposure time: 15s

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Secondary antibody - goat anti-rabbit H&L (HRP) (ab6721)

  All lanes: Western blot - Anti-CD45 antibody (Anti-CD45 antibody ab10558) at 1/500 dilution

  All lanes: Jurkat Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Predicted band size: 147 kDa

  Exposure time: 3min

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Anti-Histone H3 (tri methyl K9, phospho S10) antibody ab5819 is specific for Histone H3 phosphorylated at residue S10 and tri methylated at residue K9. The activity of the antibody is blocked by the addition of the immunizing peptide, ab15644 (lanes 3 and 4).

  All lanes: Western blot - Anti-Histone H3 (tri methyl K9, phospho S10) antibody (Anti-Histone H3 (tri methyl K9, phospho S10) antibody ab5819) at 1/500 dilution

  Lanes 1, 3, 5, 7 and 9: HeLa histones at 0.5 µg

  Lanes 10, 2, 4, 6 and 8: Colcemid-treated HeLa histones at 0.5 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 15 kDa

  Observed band size: 22 kDa, 60 kDa

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  This image is courtesy of an anonymous customer review

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Blocking Step: 5% Milk for 1 hour at 25°C.

  All lanes: Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (Anti-P70 S6 Kinase (phospho T389) antibody ab2571) at 1/250 dilution

  Lane 1: Normal Rat liver whole tissue lysate. at 20 µg

  Lane 2: Rat liver whole tissue lysate 24 hours after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg

  Lane 3: Rat liver whole tissue lysate 1 week after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 59 kDa

  Observed band size: 70 kDa

• 

  Image is courtesy of a customer review submitted by Dr Helder Fonseca.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Immunohistochemical analysis of PFA-fixed paraffin-embedded rat cardiac tissue sections, labeling Conexin 43 with Anti-Connexin 43 / GJA1 antibody ab117843 at a dilution of 1/500 incubated for 12 hours at 4°C in 1% BSA in TBS. Antigen retrival was via Tris-EDTA pH 9.0 (heat mediated). Blocking was 3% BSA incubated for 1 hour at 37°C. The secondary was ab6721 at 1/500.

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

  Rabbit polyclonal to Histone H4 acetyl K91 (Anti-Histone H4 (acetyl K91) antibody - ChIP Grade ab4627). All lanes contain 1ug of calf thymus Histone prep.

  Lane 1: Anti-Histone H4 (acetyl K91) antibody - ChIP Grade ab4627 at 1/500
  Lane 2: Anti-Histone H4 (acetyl K91) antibody - ChIP Grade ab4627 at 1/1000
  Lane 3: Anti-Histone H4 (acetyl K91) antibody - ChIP Grade ab4627 at 1/500 + 2ug acetyl K91 blocking peptide (ab10112)
  Lane 4: Anti-Histone H4 (acetyl K91) antibody - ChIP Grade ab4627 at 1/1000 + 2ug acetyl K91 blocking peptide (ab10112)

  Secondary antibody: Goat anti-rabbit (HRP) - ab6721 at 1/2000
  

  Predicted Molecular weight11KDa

  Lanes 1 and 3: Western blot - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (Anti-Histone H4 (acetyl K91) antibody - ChIP Grade ab4627) at 1/500 dilution

  Lanes 2 and 4: Western blot - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (Anti-Histone H4 (acetyl K91) antibody - ChIP Grade ab4627) at 1/1000 dilution

  All lanes: Calf thymus histone prep. at 1 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

  Predicted band size: 11 kDa

  Observed band size: 11 kDa