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Suitable for Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB. Ideal for western blot. Cited in 5847 publications.
Alternative names=Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
- Host species
Goat
- Target species
Rabbit
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Conjugation
HRP
- Applications
Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB
- Clonality
Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/120000 | Notes - |
Application ICC | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/1000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/1000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000 - 1/20000 | Notes Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283). Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances. |
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Suitable for Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB. Ideal for western blot. Cited in 5847 publications.
Alternative names=Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Key facts
- Description
Goat Anti-Rabbit IgG H&L (HRP)
- Host species
Goat
- Target species
Rabbit
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Conjugation
HRP
- Applications
Dot, IM, ELISA, ICC, IHC-Fr, IHC-P, WB
- Clonality
Polyclonal
- Pre-adsorbed
No
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate- Purification technique
Affinity purification Immunogen
- Purification notes
RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer's feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
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