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Suitable for IHC-P, ICC, ELISA, WB. Ideal for western blot. Cited in 1099 publications.


Images

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051), expandable thumbnail
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051), expandable thumbnail
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051), expandable thumbnail
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051), expandable thumbnail
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051), expandable thumbnail

Publications

  • Genes 15:2024
    FANCM Gene Variants in a Male Diagnosed with Sertoli Cell-Only Syndrome and Diffuse Astrocytoma.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Monika Logara Klarić,Tihana Marić,Lucija Žunić,Lovro Trgovec-Greif,Filip Rokić,Ana Fiolić,Ana Merkler Šorgić,Davor Ježek,Oliver Vugrek,Antonia Jakovčević,Maja Barbalić,Robert Belužić,Ana Katušić Bojanac
    PubMed 38927643
  • Aging 16:1352-13732024
    MicroRNA-141-3p reduces pulmonary hypoxia/reoxygenation injury through suppression of Beclin-1-dependent autophagy.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yanping Zhan,Lei Li,Chen Guo,Yang Zhang,Lili Zhao,Zhe Tao,Hua Zhang,Shibiao Chen
    PubMed 38261732

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

IHC-P, ICC, ELISA, WB

Clonality

Polyclonal

Abcam Recommends

Reactivity data

Application

IHC-P

Reactivity

Reacts

Dilution info

1/200.00000 - 1/5000.00000

Notes

-

Application

ICC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/10000.00000 - 1/100000.00000

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

1/2000.00000 - 1/20000.00000

Notes

Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

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Suitable for IHC-P, ICC, ELISA, WB. Ideal for western blot. Cited in 1099 publications.

Alternative names

Key facts

Description

Goat Anti-Rabbit IgG H&L (HRP)

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

IHC-P, ICC, ELISA, WB

Clonality

Polyclonal

Pre-adsorbed

No

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Isotype

IgG

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: PBS, 0.2% BSA

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Part of the AbExcel range.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

38 product images

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] ab241062) at 1/5000 dilution

    Lane 1: HEK-293 (human embryonic kidney) over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 15 µg

    Lane 2: MLi-2 (100nM for 60min) treated HEK-293 over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    The basal expression level of AKT1 (phospho S473) varies in different cell lines reported by PMID: 19372546. But to detect clear signal, treatment is strongly recommended when using this antibody.

    Blocking and Diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-AKT1 (pS473) + AKT2 (pS474) + AKT3 (pS472) antibody [EPR18853] (Anti-AKT1 (pS473) + AKT2 (pS474) + AKT3 (pS472) antibody [EPR18853] ab192623) at 1/1000 dilution

    Lane 1: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: LNCaP (Human prostate carcinoma epithelial cell) treated with 0.1 uM Calyculin A for 30 minutes whole cell lysates at 20 µg

    Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 5: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 6: HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg

    Lane 7: C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

    Lane 8: Mouse brain lysates at 20 µg

    Lane 9: Rat heart lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

    Exposure time: 50s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-ALDH3A1 antibody [EPR7405] ab128902, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ALDH3A1 antibody [EPR7405] (Anti-ALDH3A1 antibody [EPR7405] ab128902) at 1/5000 dilution

    All lanes: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    All lanes: Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (Anti-Nucleolin (phospho T84) antibody [EPR8080] ab155977) at 1/5000 dilution

    Lane 1: Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg

    Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 76 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-CXCL7/PBP antibody [EPR19780] ab206966, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CXCL7/PBP antibody [EPR19780] (Anti-CXCL7/PBP antibody [EPR19780] ab206966) at 1/1000 dilution

    All lanes: Human NAP2 active recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa

    Observed band size: 11 kDa

    Exposure time: 5s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (Catalog#Recombinant human PKC alpha protein (Active) ab55672) contains aa1-672 with GST-tag; Active human PKC gamma full length protein (Catalog#Recombinant human PKC gamma protein ab60842) contains aa1-677 with GST-tag.

    All lanes: Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] ab184746) at 1/1000 dilution

    Lane 1: Active human PKC alpha full length protein at 0.02 µg

    Lane 2: Active human PKC gamma full length protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77 kDa, 78 kDa

    Exposure time: 1s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-CASZ1 antibody [EPR23824-4] ab259847, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: Mouse liver (PMID: 16631614)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 33594190, 32060262)

    Exposure time: 81 seconds

    All lanes: Western blot - Anti-CASZ1 antibody [EPR23824-4] (Anti-CASZ1 antibody [EPR23824-4] ab259847) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: Mouse E18 heart tissue lysate at 20 µg

    Lane 3: Mouse P20 retina tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 190 kDa

    Observed band size: 150 kDa, 250 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] ab75991, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] ab75991) at 1/1000 dilution

    Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes, whole cell lysate at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    All lanes: Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] ab151728) at 1/2000 dilution

    All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-alpha 1 Fetoprotein antibody [EPR26548-19] ab290637, the same antibody clone in different buffer formulation.

    Blocking and dilution buffer and concentration was 5% NFDM/TBST.

    All lanes: Western blot - Anti-alpha 1 Fetoprotein antibody [EPR26548-19] (Anti-alpha 1 Fetoprotein antibody [EPR26548-19] ab290637) at 1000 Cells

    Lane 1: Untreated Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Hepa1-6 treated with 300ng/ml Brefeldin A for 24h whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa

    Observed band size: 70 kDa

    Exposure time: 37s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-PU.1/Spi1 antibody [EPR3158Y] ab76543, the same antibody clone in a different buffer formulation.

    PU.1 is a 37-42 kDa member of the ets family of transcription factors. The molecule weight observed is consistent with what has been described in the literature (PMID: 28362429, 31586032, 28586009).

    All lanes: Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (Anti-PU.1/Spi1 antibody [EPR3158Y] ab76543) at 1/1000 dilution

    Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 2: THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 3: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa

    Observed band size: 37-42 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was produced using Anti-LYVE1 antibody [RM1008] ab281587, the same clone but in a different formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

    Exposure time: 37 seconds

    Lanes 1 and 3: Mouse lung lysate at 20 µg

    Lane 2: Mouse brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Exposure times: Lanes 1,2: 1 second; Lanes 3-6: 5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RPS6 (phospho S240 + S244) antibody [EPR20770] (Anti-RPS6 (phospho S240 + S244) antibody [EPR20770] ab215214) at 1/5000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma cell line) grown in serum-free media overnight, whole cell lysate at 20 µg

    Lane 2: MCF7 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes, whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast cell line) grown in serum-free media for 4 hours, whole cell lysate at 20 µg

    Lane 4: NIH/3T3 grown in serum-free media for 4 hours, then grown in 20% FBS media for 30 minutes, whole cell lysate at 20 µg

    Lane 5: PC-12 (rat adrenal gland pheochromocytoma cell line) grown in serum-free media overnight, whole cell lysate at 20 µg

    Lane 6: PC-12 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa

    Observed band size: 32 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was produced using Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control ab76020, the same antibody clone in a different formulation

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    We suggest not to boil the sample after lysis.

    All lanes: Western blot - Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free (Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free ab167390) at 1/100000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared from RIPA lysis method at 15 µg

    Lane 2: HeLa whole cell lysate prepared from 1% SDS HOT lysis method at 15 µg

    Lane 3: HeLa whole cell lysate prepared from RIPA lysis method at 15 µg

    Lane 4: HeLa whole cell lysate prepared from 1%SDS HOT lysis method at 15 µg

    Lane 5: Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysate prepared from RIPA lysis method at 15 µg

    Lanes 6 and 8: Raw264.7 whole cell lysate prepared from 1%SDS HOT lysis method at 15 µg

    Lane 7: Raw264.7 whole cell lysate prepared from RIPA lysis method at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 113 kDa

    Observed band size: 100 kDa

    Exposure time: 10s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Exposure times: Lane 1: 1 minute; Lane 2: 20 seconds; Lane 3: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    All lanes: Western blot - Anti-Thrombomodulin antibody [EPR18217-209] (Anti-Thrombomodulin antibody [EPR18217-209] ab230010) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg

    Lane 3: Mouse placenta tissue lysate at 10 µg

    Secondary

    Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Lanes 2 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa

    Observed band size: 105 kDa, 75 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (Anti-CRISPR-Cas9 antibody [EPR19633] ab202657) at 1/20000 dilution

    Lane 1: Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag at 20 µg

    Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-Myc tag at 20 µg

    Lane 4: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag at 20 µg

    Lane 5: Empty vector with Myc-His tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 6: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) with Myc-His tag at 20 µg

    Lane 7: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag at 20 µg

    Lane 8: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with Myc-His tag at 20 µg

    Lane 9: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 Cells

    Predicted band size: 164 kDa

    Exposure time: 5s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    All lanes: Western blot - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] ab76238) at 1/20000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

    Lane 3: C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa

    Observed band size: 42 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-VIRMA/KIAA1429 antibody [EPR24279-23] ab271136, the same antibody clone in a different buffer formulation.

    The observed MW are consistent with what has been described in the literature (PMID: 34490238).

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (Anti-VIRMA/KIAA1429 antibody [EPR24279-23] ab271136) at 1/1000 dilution

    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate at 20 µg

    Lane 3: T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

    Lane 4: C2C12 (mouse myoblasts), whole cell lysate at 20 µg

    Lane 5: Mouse liver cancer lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 202 kDa

    Observed band size: 202 kDa

    Exposure time: 26s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] ab76296, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] ab76296) at 1/1000 dilution

    Lane 1: Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 2: C6 treated with 10ng/ml TGF beta 3 and 50µM MG-132 for 30 min whole cell lysate at 15 µg

    Lane 3: C6 treated with 10ng/ml TGF beta 3 and 50µM MG-132 for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 52 kDa

    Exposure time: 7s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-CYP1A1 antibody ab124295 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    All lanes: Western blot - Anti-CYP1A1 antibody (Anti-CYP1A1 antibody ab124295) at 1 µg/mL

    Lanes 1 and 3: Lung (Human) Tissue Lysate at 10 µg

    Lanes 2 and 4: Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa

    Observed band size: 48 kDa, 55 kDa, 73 kDa

    Exposure time: 90s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Anti-CD130 (gp130) antibody [EPR24557-50] ab283685 was shown to specifically react with IL6ST (CD130/gp130) in wild-type A549 cells. Loss of signal was observed when knockout cell line Human IL6ST (CD130/gp130) knockout A549 cell line ab266938 (knockout cell lysate Human IL6ST (CD130/gp130) knockout A549 cell lysate ab257207) was used. Wild-type and IL6ST (CD130/gp130) knockout samples were subjected to SDS-PAGE. Anti-CD130 (gp130) antibody [EPR24557-50] ab283685 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated for 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 70 seconds

    All lanes: Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (Anti-CD130 (gp130) antibody [EPR24557-50] ab283685) at 1/1000 dilution

    Lane 1: Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: IL6ST (CD130/gp130) knockout A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 104 kDa

    Observed band size: 130 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RNA polymerase beta antibody [EPR18704] (Anti-RNA polymerase beta antibody [EPR18704] ab191598) at 1/2000 dilution

    All lanes: E.coli whole cell lysates at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 150 kDa

    Observed band size: 150 kDa

    Exposure time: 30s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Active human PKC alpha full length protein (Catalog#Recombinant human PKC alpha protein (Active) ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein(Catalog#Recombinant human PKC beta 1 protein ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein(Catalog#Recombinant human PKC beta 2 protein ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein(Catalog#Recombinant human PKC gamma protein ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein(Catalog#Recombinant human PKC delta protein ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein(Catalog#Recombinant human PKC eta protein ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein(Catalog#Recombinant human PKC epsilon protein ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein(Catalog#Recombinant human PKC theta/PRKCQ protein ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein(Catalog#Recombinant human PKC mu/PKD protein ab60873) contains aa1-912 with GST-tag.

    Blocking/Diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-PKC antibody [EPR16898] (Anti-PKC antibody [EPR16898] ab179522) at 1/2000 dilution

    Lane 1: Active human PKC alpha full length protein at 0.02 µg

    Lane 2: Active human PKC beta 1 full length protein at 0.02 µg

    Lane 3: Active human PKC beta 2 full length protein at 0.02 µg

    Lane 4: Active human PKC gamma full length protein at 0.02 µg

    Lane 5: Active human PKC delta full length protein at 0.02 µg

    Lane 6: Active human PKC eta full length protein at 0.02 µg

    Lane 7: Active human PKC epsilon full length protein at 0.02 µg

    Lane 8: Active human PKC theta full length protein at 0.02 µg

    Lane 9: Active human PKC mu full length protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77 kDa

    Exposure time: 2s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Netrin 1 antibody [EPR5428] (Anti-Netrin 1 antibody [EPR5428] ab126729) at 1/500 dilution

    Lane 1: Rat brain lysates prepared in RIPA lysis method at 20 µg

    Lane 2: Rat brain lysates prepared in 1%SDS Hot lysis method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 67 kDa

    Observed band size: 75 kDa

    Exposure time: 180s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    All lanes: Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (Anti-DNA Polymerase beta antibody [EPR12722(B)] ab175197) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 4: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: Mouse brain lysate at 20 µg

    Lane 6: Mouse spleen lysate at 20 µg

    Lane 7: Rat brain lysate at 20 µg

    Lane 8: Rat spleen lysate

    Lane 9: C6 (Rat glial tumor glial cell) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 38 kDa

    Observed band size: 38 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This antibody shows low affinity on HeLa cells and detects a strong non-specific band around 75 KD.

    All lanes: Western blot - Anti-ARF6 antibody [EPR8357] (Anti-ARF6 antibody [EPR8357] ab131261) at 1/1000 dilution

    All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa

    Observed band size: 18 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    All lanes: Western blot - Anti-DDX17 antibody [EPR13807(B)] (Anti-DDX17 antibody [EPR13807(B)] ab180190) at 1/1000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Rat brain lysate at 20 µg

    Lane 4: Rat testis lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking buffer: 5% NFDM /TBST

    All lanes: Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] (Anti-RNF185+RNF5/NG2 antibody [EPR14070] ab181227) at 1/1000 dilution

    Lane 1: Human RNF185 full-length ORF recombinant protein with GST-tag at N-terminal at 0.01 µg

    Lane 2: Human RNF5 full-length ORF recombinant protein with GST-tag at N-terminal at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 20 kDa

    Observed band size: 47.45 kDa

    Exposure time: 1s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times:

    Lanes 1-3: 10 seconds.

    Lane 4: 8 seconds.

    All lanes: Western blot - Anti-Histone H4 (mono methyl R92) antibody [EPR21782] (Anti-Histone H4 (mono methyl R92) antibody [EPR21782] ab219797) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

    Lane 2: C6 (rat glioma cell line) whole cell lysate at 20 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa

    Observed band size: 11 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    The bands detected are consistent with what have been described in the literatures (PMID: 11741954, 27033701). Full-length Integrin alpha V: 150kDa; Integrin alpha V light chain: 27-30kDa

    All lanes: Western blot - Anti-Integrin alpha V antibody [EPR5583] (Anti-Integrin alpha V antibody [EPR5583] ab124968) at 1/1000 dilution

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 4: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 116 kDa

    Observed band size: 150 kDa, 27-30 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-EpCAM antibody [RM1016] ab282457, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HeLa (PMID: 31806375), NIH/3T3 (PMID: 23264216).

    The MW observed is consistent with the literature: PMID 19136966; PMID 23618806. The band between 12-15kDa has been documented in literature as the cleaved form of EpCAM (PMID: 23409978).

    Exposure time: 8 seconds.

    All lanes: Western blot - Anti-EpCAM antibody [RM1016] - BSA and Azide free (Anti-EpCAM antibody [RM1016] - BSA and Azide free ab283866) at 1/1000 dilution

    Lane 1: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: Human colon lysate at 20 µg

    Lane 6: Mouse colon lysate at 20 µg

    Lane 7: Rat colon lysate at 20 µg

    Lane 8: Mouse kidney lysate at 20 µg

    Lane 9: Rat kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 12 kDa, 36 kDa, 40 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (Catalog#Recombinant human PKC alpha protein (Active) ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (Catalog#Recombinant human PKC beta 1 protein ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (Catalog#Recombinant human PKC beta 2 protein ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (Catalog#Recombinant human PKC gamma protein ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (Catalog#Recombinant human PKC delta protein ab60844) contains aa1-676 with GST-tag; Active human PKC mu full length protein (Catalog#Recombinant human PKC mu/PKD protein ab60873) contains aa1-912 with GST-tag.

    All lanes: Western blot - Anti-PKC antibody [EPR16791] (Anti-PKC antibody [EPR16791] ab179523) at 1/2000 dilution

    Lane 1: Active human PKC alpha full length protein at 0.02 µg

    Lane 2: Active human PKC beta 1 full length protein at 0.02 µg

    Lane 3: Active human PKC beta 2 full length protein at 0.02 µg

    Lane 4: Active human PKC gamma full length protein at 0.02 µg

    Lane 5: Active human PKC delta full length protein at 0.02 µg

    Lane 6: Active human PKC mu full length protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77 kDa

    Exposure time: 3min

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-beta Catenin non-phospho S37/T41 antibody [EPR23969-131] ab246504, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time 92 seconds

    An extra band around 45 kDa was observed.

    All lanes: Western blot - Anti-beta Catenin non-phospho S37/T41 antibody [EPR23969-131] (Anti-beta Catenin non-phospho S37/T41 antibody [EPR23969-131] ab246504) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: LLC1 (mouse lung carcinoma ) whole cell lysate at 20 µg

    Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 85 kDa

    Observed band size: 95 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    The bands observed are consistent with what have been described in PMID 30649915 as isoforms of p63.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p63 antibody [EPR5701] ab124762).

    All lanes: Western blot - Anti-p63 antibody [EPR5701] (Anti-p63 antibody [EPR5701] ab124762) at 1/1000 dilution

    Lane 1: HaCaT (Human skin keratinocyte) whole cell lysates at 20 µg

    Lane 2: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: Mouse skin lysates at 20 µg

    Lane 4: Mouse thymus lysates at 20 µg

    Lane 5: Mouse bladder lysates at 20 µg

    Lane 6: Rat skin lysates at 20 µg

    Lane 7: Rat bladder lysates at 20 µg

    Lane 8: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa

    Observed band size: 37-75 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 10809957).

    Negative control: SH-SY5Y (PMID: 26749374), K562 (PMID: 21037579).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD1d antibody [EPR22526-9] (Anti-CD1d antibody [EPR22526-9] ab256344) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 2: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

    Lane 3: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa

    Observed band size: 48 kDa

    Exposure time: 37s

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as a blocking and diluting buffer and concentration.

    Negative control: liver, spleen(PMID: 1309946)

    Samples are non-boiled as boiling may cause protein aggregates.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] (Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 226 kDa

    Observed band size: 250 kDa

    Exposure time: 3min

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    This data was developed using 259919, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: HL-60 (PMID: 10445843 )

    The two reactive bands represent different isoforms

    Exposure time: 37 seconds.

    All lanes: Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (Anti-L3MBTL1 antibody [EPR23810-121] ab259919) at 1/1000 dilution

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 7: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa

    Observed band size: 100 kDa

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SF3B3 antibody [EPR18441] (Anti-SF3B3 antibody [EPR18441] ab209403) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 136 kDa

    Observed band size: 136 kDa

    Exposure time: 8s

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