Goat Anti-Rabbit IgG H&L (HRP polymer)

40 product images

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  Sonzogni et al PLoS Biol. 2018 Jun 20;16(6):e2004049. doi: 10.1371/journal.pbio.2004049. eCollection 2018 Jun. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Representative images IHC for Ki67 (upper panel) and CC3 (lower panel); scale bar 50 μm.

  The paraffin sections were deparaffinized and rehydrated, followed by antigen retrieval using sodium citrate buffer (pH 6). The sections were then incubated with 3% hydrogen peroxide to block endogenous peroxidase activity for 10 minutes at room temperature. After 3 washes with TBS, the sections were incubated with 2% bovine serum albumin for 1 hour at room temperature.

  Slides were then incubated separately with rabbit anti-Ki67 and rabbit anti-cleaved caspase 3 overnight at 4°C. The slides were washed 3 times and incubated with goat anti-rabbit HRP polymer (1:1, Abcam ab214880) for 2 hours at room temperature. Samples were washed thrice and then developed, counterstained with hematoxylin and then dehydrated before mounting the slides with permount.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of ER/PR positive breast cancer tissue stained for ER and detected with ab214880.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ki67 with Anti-Ki67 antibody [SP6] ab16667 at 1/200 (0.145 µg/ml) followed by a ready to use LGoat Anti-Rabbit IgG H&L (HRP polymer) secondary antibody (ab214880). Low expression: positive staining only on proliferating cells (arrow) of human liver. The section was incubated with Anti-Ki67 antibody [SP6] ab16667 at 4°C overnight and counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ki67 with Anti-Ki67 antibody [SP6] ab16667 at 1/200 (0.145 µg/ml) followed by a ready to use LGoat Anti-Rabbit IgG H&L (HRP polymer) secondary antibody (ab214880). Low expression: positive staining only on proliferating cells (arrow) of mouse kidney. The section was incubated with Anti-Ki67 antibody [SP6] ab16667 at 4°C overnight and counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded (A) Non-perfused fix tissue labeling Transferrin with Anti-Transferrin antibody [EPR28141-177] - Serum Loading Control ab313583 at 1/100 (5.21 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on hepatocytes and plasma of (A) non-perfused fixed mouse liver; only positive on hepatocytes of (B) perfused fixed mouse liver.The section was incubated with Anti-Transferrin antibody [EPR28141-177] - Serum Loading Control ab313583 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded (A) Non-perfused fix tissue labeling Transferrin with Anti-Transferrin antibody [EPR28141-177] - Serum Loading Control ab313583 at 1/100 (5.21 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on plasma of (A) non-perfused fixed mouse cardiac muscle, no staining on (B) perfused fixed mouse cardiac muscle.The section was incubated with Anti-Transferrin antibody [EPR28141-177] - Serum Loading Control ab313583 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse perfused fixed tissue labeling Transferrin with Anti-Transferrin antibody [EPR28141-177] - Serum Loading Control ab313583 at 1/100 (5.21 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control: No staining on mouse perfused fixed skeletal muscle.The section was incubated with Anti-Transferrin antibody [EPR28141-177] - Serum Loading Control ab313583 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  IHC image of Anti-Ki67 antibody [SP6] ab16667 staining Ki67 in a section of formalin-fixed paraffin-embedded Human colon carcinoma tissue. The section incubated with Anti-Ki67 antibody [SP6] ab16667 at 1/200 (0.145 μg/ml) dilution and ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) was used as secondary antobody. Positive staining on human colon carcinoma. The section was incubated with Anti-Ki67 antibody [SP6] ab16667 at 4°C overnight. The section was counterstained with haematoxylin.
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Digoxigenin with Anti-Digoxigenin antibody [EPR26829-63] ab320717 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Positive staining on (A) human tonsil incubated with mouse monoclonal PD-1 antibody conjugated to Digoxigenin, and no staining on (B) human tonsil incubated with mouse monoclonal PD-1 antibody and (C) human tonsil incubated with PBS, then all the sections were incubated with Anti-Digoxigenin antibody [EPR26829-63] ab320717 at 4°C overnight.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRPA1/TSA with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Negative control: no staining on human liver. The section was incubated with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 4°C overnight.
  
  Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TRPA1/TSA with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Negative control: no staining on human lung. The section was incubated with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 4°C overnight.
  
  Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Muscularis of human colon tissue labeling TRPA1/TSA with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Positive staining on myenteric ganglia of human colon (PMID: 28478727). The section was incubated with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 4°C overnight.
  
  Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human dorsal root ganglion (DRG) tissue labeling TRPA1/TSA with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Positive staining on human dorsal root ganglion (DRG). The section was incubated with Anti-TRPA1/TSA antibody [EPR26211-139] ab320715 at 4°C overnight.
  
  Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human testis. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Dnmt1 with Anti-Dnmt1 antibody [RM1192] ab317845 at 1/100 (5.31 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Positive staining on rat testis. The section was incubated with Anti-Dnmt1 antibody [RM1192] ab317845 at 4°C overnight.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Dnmt1 with Anti-Dnmt1 antibody [RM1192] ab317845 at 1/100 (5.31 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Positive staining on mouse testis. The section was incubated with Anti-Dnmt1 antibody [RM1192] ab317845 at 4°C overnight.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Dnmt1 with Anti-Dnmt1 antibody [RM1192] ab317845 at 1/500 (1.062 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Positve staining on human tonsil. The section was incubated with Anti-Dnmt1 antibody [RM1192] ab317845 at 4°C overnight.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling 5HT7 Receptor with Anti-5HT7 Receptor antibody [EPR28676-1] ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Negative control: No staining in mouse liver.
  
  The section was incubated with Anti-5HT7 Receptor antibody [EPR28676-1] ab317565 at 4°C overnight.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling 5HT7 Receptor with Anti-5HT7 Receptor antibody [EPR28676-1] ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Positive staining in mouse hippocampus.
  
  The section was incubated with Anti-5HT7 Receptor antibody [EPR28676-1] ab317565 at 4°C overnight.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling 5HT7 Receptor with Anti-5HT7 Receptor antibody [EPR28676-1] ab317565 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Positive staining in mouse cerebrum (PMID: 28690143).
  
  The section was incubated with Anti-5HT7 Receptor antibody [EPR28676-1] ab317565 at 4°C overnight.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Fibrillarin with Anti-Fibrillarin antibody [RM1082] ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on rat liver. The section was incubated with Anti-Fibrillarin antibody [RM1082] ab314506 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Fibrillarin with Anti-Fibrillarin antibody [RM1082] ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on mouse testis. The section was incubated with Anti-Fibrillarin antibody [RM1082] ab314506 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Fibrillarin with Anti-Fibrillarin antibody [RM1082] ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on mouse liver. The section was incubated with Anti-Fibrillarin antibody [RM1082] ab314506 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Fibrillarin with Anti-Fibrillarin antibody [RM1082] ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on human tonsil The section was incubated with Anti-Fibrillarin antibody [RM1082] ab314506 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control: no staining on human cardiac muscle. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human lung carcinoma. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human placenta. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Low expression: weakly nuclear staining on human liver. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nuclear staining on human lung cancer. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TRIP12/ULF with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nuclear staining on human testis. The section was incubated with Anti-TRIP12/ULF antibody [EPR27062-86] ab313628 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Tissue Microarrays stained for "Anti-Ki67 antibody [SP6]” using "Anti-Ki67 antibody [SP6] ab16667" at 1/200 dilution (0.145 μg/ml) in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. Taking human colon tissue as an example, Ki67 is barely expressed or the expression level is very low in normal colon tissue, and the IHC test result is usually negative. While the expression of Ki67 can be upregulated in the proliferating cells of colon tissue, and the IHC test result could be positive.
  The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0). The sections were incubated with Anti-Ki67 antibody [SP6] ab16667 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Hematoxylin was used as the counter stain.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded mouse lymph nodes tissue labeling CD23 with Anti-CD23 antibody [EPR26479-9] ab302526 at 1/100 dilution (4.67 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) as secondary antibody. Positive staining on mouse lymph nodes. The section was incubated with Anti-CD23 antibody [EPR26479-9] ab302526 at 4°C overnight. Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody without primary.

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue to label CD23 with Anti-CD23 antibody [EPR26479-9] ab302526 at 1/100 dilution (4.67 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

  Negative control: no staining on mouse cardiac muscle. The section was incubated with Anti-CD23 antibody [EPR26479-9] ab302526 at 4°C overnight. Counterstained with Hematoxylin.

  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle showing negative staining with purified Anti-CD27 antibody [EPR8569] ab131254 at 1/1800. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ab214880, a Goat Anti-Rabbit IgG H&L (HRP polymer) was used as the secondary antibody (1/500).
  
  Counterstained with hematoxylin.
  
  Negative control: No staining on human skeletal muscle. The section was incubated with Anti-CD27 antibody [EPR8569] ab131254 at 4°C overnight.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue labelling GSDMD with Anti-GSDMD antibody [EPR20859] ab219800 at 1/1000 (0.542 μg/ml) followed by ab214880 at a ready to use dilution. Low expression tissue: weak staining on mouse lung. The section was incubated with Anti-GSDMD antibody [EPR20859] ab219800 at 4°C overnight. Counterstained with hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ab214880 at a ready to use dilution.
  
  Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Tissue Microarrays stained for "Anti-Ki67 antibody [SP6]” using "Anti-Ki67 antibody [SP6] ab16667" at 1/200 dilution (0.145 μg/ml) in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0). The sections were incubated with Anti-Ki67 antibody [SP6] ab16667 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Hematoxylin was used as the counter stain.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human astrocytoma labelling Pan Trk with Anti-Pan Trk antibody [EPR17341-4] ab246551 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880).
  
  
  Positive staining on human astrocytoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma labelling Niemann Pick C2 with Anti-Niemann Pick C2 antibody [EPR19993-145-1] ab218192 at 1/8000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880).
  
  Positive staining on Human breast carcinoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)

  Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with Anti-CD10 antibody [EPR22867-118] ab256494 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880).
  
  Positive staining on Human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).