Goat Anti-Rabbit IgG H&L (HRP) preadsorbed

15 product images

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  Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with Anti-Claudin 4 antibody ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. DAB Substrate Kit ab64238 at 1/50 was used to develop the histological signal.
  

  Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.

  Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  All lanes: Western blot - Anti-hnRNP U/p120 antibody (Anti-hnRNP U/p120 antibody ab20666) at 1 µg/mL

  All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 91 kDa

  Observed band size: 117 kDa

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Lanes 1 to 2
  Lane 1: 5μg per lane Mouse Brain 1/500
  Lane 2: 5μg per lane Mouse Brain 1/1000
  Exposure time: 3 minutes
  Expected molecular weight: 78kDa
  
  Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
  ab7090 (1/5000)

  We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
  

  We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.

  Lane 1: Western blot - Anti-CPEB3 antibody (Anti-CPEB3 antibody ab10883) at 1/500 dilution

  Lane 2: Western blot - Anti-CPEB3 antibody (Anti-CPEB3 antibody ab10883) at 1/1000 dilution

  All lanes: Mouse Brain lysate at 5 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

  Predicted band size: 76 kDa

  Exposure time: 3min

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  All lanes: Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (Anti-HDAC1 antibody - Nuclear Loading Control ab19845) at 1 µg/mL

  Lane 1: HeLa whole cell lysate at 20 µg

  Lane 2: HeLa whole cell lysate at 20 µg with Human HDAC1 peptide (ab20434)

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

  Predicted band size: 55 kDa

  Observed band size: 60 kDa

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  ICC/IF image of Anti-Prohibitin antibody - Mitochondrial Marker ab28172 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (Anti-Prohibitin antibody - Mitochondrial Marker ab28172, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (Anti-COX IV antibody - Mitochondrial Loading Control ab16056) at 1 µg/mL

  Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg

  Lane 2: Human liver tissue lysate - total protein (ab29889) at 10 µg

  Lane 3: Human heart tissue lysate - total protein (ab29431) at 10 µg

  Lane 4: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/3000 dilution

  Performed under reducing conditions.

  Predicted band size: 19 kDa

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Anti-Histone H4 (biotinylated K5) antibody ab17343 specifically recognises biotinylated histone H4 K5 at 11 kDa, which is blocked using the immunising peptide (ab17632) but not the corresponding unmodified peptide (ab15825).

  All lanes: Western blot - Anti-Histone H4 (biotinylated K5) antibody (Anti-Histone H4 (biotinylated K5) antibody ab17343) at 1 µg/mL

  Lane 1: HeLa nuclear extract at 20 µg

  Lane 2: HeLa nuclear extract at 20 µg with Human Histone H4 (biotinylated K5) peptide (ab17632)

  Lane 3: HeLa nuclear extract at 20 µg with Human Histone H4 peptide (ab15825)

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 11 kDa

  Observed band size: 11 kDa

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  All lanes: Western blot - Anti-pan Cadherin antibody (Anti-pan Cadherin antibody ab16505) at 1 µg/mL

  Lane 1: Human heart lysate at 20 µg

  Lane 2: Mouse heart lysate at 20 µg

  Lane 3: Rat heart lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 100 kDa

  Observed band size: 125-140 kDa, 40 kDa, 65 kDa, 75 kDa, 90 kDa

  Exposure time: 1min

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  This image is courtesy of an anonymous customer review.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Anti-Osteoprotegerin antibody ab9986 staining osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA and 5% normal goat serum in PBS for 30 minutes at 22°C; Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab7090) (1/300) was used as the secondary antibody.

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.

  Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.

  A 4-20% Criterion gel for SDS-PAGE was used.

  All lanes: Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody ab2479) at 1/1000 dilution

  Lanes 1 and 5: mock treated mouse cardiac myocyte lysate at 12 µL

  Lane 2: insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µL

  Lane 3: insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µL

  Lane 4: insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µL

  Lane 6: CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µL

  Lane 7: CLA treated (20 nM, 45min) mouse cardiac myocyte lysate

  Lane 8: CLA treated (100 nM, 45min) mouse cardiac myocyte lysate

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

  Predicted band size: 84 kDa

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Western blot of EMSY on MCF-7 cell lysate.

  Lane 1: ab4579 at 1/500
  Lane 2: Anti-EMSY antibody ab123 at 1/500.
  
  Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both Anti-EMSY antibody ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
  
  Secondary ab Lane 1: Rabbit polyclonal to Goat IgG H&L (HRP) Rabbit Anti-Goat IgG H&L (HRP) ab6741 (1/5000)
  Exposure time: 3 min.

  Secondary ab Lane 2: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
  Exposure time: 10 sec.

  Lysate at 20μg/lane.

  Lane 1 - Exposure time : 3 min.
  Lane 2 - Exposure time : 10 sec.
  
  Variability in the size at which EMSY runs by Western blot has been exper

  Lane 1: Anti-EMSY antibody (ab4579) at 1/500 dilution

  Lane 2: Western blot - Anti-EMSY antibody (Anti-EMSY antibody ab123) at 1/500 dilution

  All lanes: MCF-7 cell lysate at 20 µg

  Secondary

  Lane 1: Western blot - Rabbit Anti-Goat IgG H&L (HRP) (Rabbit Anti-Goat IgG H&L (HRP) ab6741) at 1/5000 dilution

  Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 141 kDa

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  The observed band size is consistent with the expected size of the Pin1 partial recombinant protein used (amino acids 64 - 163 of the human Pin1 sequence, with GST).

  Lane 1: Marker

  Lanes 2 - 3: Western blot - Anti-Pin1 antibody (Anti-Pin1 antibody ab12107) at 1 µg/mL

  Lane 2: pin1 Recombinant 0.1ug

  Lane 3: pin1 Recombinant 0.5ug

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 18 kDa

  Observed band size: ~34 kDa

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  ELISA - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  ELISA results of ab7090 against purified Goat Anti-Rabbit IgG Antibody HRP Conjugated MX10. Each well was coated in duplicate with 10 µg of Rabbit IgG [Red Line], Bovine IgG (p/n 001-0102) [Green Line], Chicken IgG [Blue Line], Goat IgG [Purple Line], Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep tested with similar results. The working dilution for Rabbit IgG is 1/109,000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel and TMB substrate.

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  Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Western blot analysis of secondary antibody ab7090 used at 1/40000 dilution to detect a rabbit primary antibody. Anti-p27 antibody showed detection of 0.1 µg of recombinant p27 protein. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose and blocked with 1% BSA-TTBS overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/40000 dilution

  Lane 2: MBP-p27 fusion protein

  Lane 3: MBP

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  Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  Dot blot analysis of ab7090. Secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (250ng starting total load) co-incubated with HRP conjugated Goat anti Rabbit IgG and Dylight 649 conjugated goat anti Rabbit. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649); (B) LiCor Odyssey Reader (700 nm); (C) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec); (D) Incubated with TMB substrate TMBM for 5 minutes and scanned; (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.

  All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

  All lanes: Rabbit IgG