Goat Anti-Rabbit IgG H&L (HRP) preadsorbed

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The band observed at 75 kDa could potentially be a cleaved form of GRP78 BiP due to the presence of a 18 amino acid signal peptide.

All lanes:

Western blot - Anti-GRP78 BiP antibody (<a href='/en-us/products/primary-antibodies/grp78-bip-antibody-ab32618'>ab32618</a>) at 1 µg/mL

Lane 1:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 2:

CHO-K1 cell lysate Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 72 kDa

Observed band size: 75 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-SOX5 antibody (<a href='/en-us/products/primary-antibodies/sox5-antibody-ab94396'>ab94396</a>) at 1 µg/mL

All lanes:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 84 kDa

Observed band size: 24 kDa,28 kDa,55 kDa,84 kDa

true

Exposure time: 5min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124296 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Ceramide glucosyltransferase antibody (<a href='/en-us/products/primary-antibodies/ceramide-glucosyltransferase-antibody-ab124296'>ab124296</a>) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

Spleen (Mouse) Tissue Lysate at 10 µg

Lane 5:

Spleen (Rat) Tissue Lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

A375 (Human melanoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 45 kDa

Observed band size: 12 kDa,36 kDa,48 kDa,68 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-PSPC1 antibody (<a href='/en-us/products/primary-antibodies/pspc1-antibody-ab104238'>ab104238</a>) at 1 µg/mL

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 2:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 59 kDa

Observed band size: 62 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-Claudin 4 antibody (<a href='/en-us/products/primary-antibodies/claudin-4-antibody-ab15104'>ab15104</a>) at 1 µg/mL

All lanes:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 22 kDa

Observed band size: 125 kDa,20 kDa,34 kDa,35 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

ab45939 recognizes full length recombinant Human vimentin (ab73843) which has an expected molecular weight of 54 kDa.

All lanes:

Western blot - Anti-Vimentin antibody - Cytoskeleton Marker (<a href='/en-us/products/primary-antibodies/vimentin-antibody-cytoskeleton-marker-ab45939'>ab45939</a>) at 1 µg/mL

Lane 1:

Recombinant Human Vimentin protein (<a href='/en-us/products/unavailable/recombinant-human-vimentin-protein-ab73843'>ab73843</a>) at 0.1 µg

Lane 2:

Recombinant Human Vimentin protein (<a href='/en-us/products/unavailable/recombinant-human-vimentin-protein-ab73843'>ab73843</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 53 kDa

Observed band size: 54 kDa

true

Exposure time: 10s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-Cytokeratin 10 antibody (<a href='/en-us/products/primary-antibodies/cytokeratin-10-antibody-ab111447'>ab111447</a>) at 1 µg/mL

All lanes:

Human skin tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skin-tissue-lysate-total-protein-ab30166'>ab30166</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 59 kDa

Observed band size: 62 kDa

true

Exposure time: 16min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The band observed at 16 kDa could potentially be a cleaved form of ISG15 due to the presence of an 8 amino acid propeptide.

All lanes:

Western blot - Anti-ISG15 antibody (<a href='/en-us/products/primary-antibodies/isg15-antibody-ab14374'>ab14374</a>) at 1/500 dilution

All lanes:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 18 kDa

true

Exposure time: 12min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-non-muscle Myosin IIA antibody (<a href='/en-us/products/primary-antibodies/non-muscle-myosin-iia-antibody-ab75590'>ab75590</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate at 10 µg

Lane 4:

HEK-293 (Human embryonic kidney cell line) whole cell lysate at 10 µg

Lane 5:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 227 kDa

Observed band size: 100 kDa,227 kDa,35 kDa,45 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.

All lanes:

Western blot - Anti-Furin antibody (<a href='/en-us/products/primary-antibodies/furin-antibody-ab3467'>ab3467</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 87 kDa

Observed band size: 63 kDa

true

Exposure time: 90s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates.

All lanes:

Western blot - Anti-S1P antibody (<a href='/en-us/products/primary-antibodies/s1p-antibody-ab59870'>ab59870</a>) at 1 µg/mL

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 118 kDa

true

Exposure time: 16min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

GPR54 receptor contains a number of potential glycosylation sites (SwissProt) which may explain the migration pattern observed.

All lanes:

Western blot - Anti-GPR54 antibody (<a href='/en-us/products/primary-antibodies/gpr54-antibody-ab100896'>ab100896</a>) at 1 µg/mL

All lanes:

Human spinal cord tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spinal-cord-tissue-lysate-total-protein-ab29188'>ab29188</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 42 kDa

true

Exposure time: 20min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

A doublet was observed in Daudi, Ramos and Raji whole cell lysates (lanes 1, 4 and 5). This data suggests that ab23423 might react with both cIAP1 and cIAP2 proteins. The predicted molecular weights of cIAP1 and cIAP2 are 69-kDa and 68-kDa respectively. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-cIAP2 antibody (<a href='/en-us/products/primary-antibodies/ciap2-antibody-ab23423'>ab23423</a>) at 1 µg/mL

Lane 1:

Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg/mL

Lane 2:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg/mL

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg/mL

Lane 4:

Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg/mL

Lane 5:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg/mL

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg/mL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 68 kDa

Observed band size: 150 kDa,35 kDa,73 kDa,75 kDa

true

Exposure time: 8min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-Histone H2A antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h2a-antibody-chip-grade-ab88770'>ab88770</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 100 kDa,17 kDa,36 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID : 21055983).

All lanes:

Western blot - Anti-NFKBIL2 antibody (<a href='/en-us/products/primary-antibodies/nfkbil2-antibody-ab101898'>ab101898</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 151 kDa

Observed band size: 175 kDa,43 kDa,67 kDa,80 kDa

true

Exposure time: 20min

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data). Some publications suggest that the human protein should be detected at 47 kDa (PMID 12011435). Our data shows that ab26405 detects a band at 36 kDa in human brain, which is similar to that seen in other publications [PMID : 14680841, PMID : 18159220 (expression profile)]. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-MafA antibody (<a href='/en-us/products/primary-antibodies/mafa-antibody-ab26405'>ab26405</a>) at 1 µg/mL

All lanes:

Brain (Human) Tissue Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

We hypothesize that the 25 and 30 kDa bands represent the heavy chain and mature form of Cathepsin B, respectively. Abcam welcomes customer feedback.

All lanes:

Western blot - Anti-Cathepsin B antibody (<a href='/en-us/products/primary-antibodies/cathepsin-b-antibody-ab92955'>ab92955</a>) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Lane 3:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 4:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 25 kDa,30 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The band observed at 14 kDa could potentially be a cleaved form of REG1 alpha due to the presence of a 25 amino acid signal peptide.

All lanes:

Western blot - Anti-REG1 alpha antibody (<a href='/en-us/products/primary-antibodies/reg1-alpha-antibody-ab47099'>ab47099</a>) at 1 µg/mL

All lanes:

Human pancreas tissue lysate - total protein (<a href='/en-us/products/unavailable/human-pancreas-tissue-lysate-total-protein-ab29816'>ab29816</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 19 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

BSCL2/Seipin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-BSCL2/Seipin antibody (<a href='/en-us/products/primary-antibodies/bscl2-seipin-antibody-ab106793'>ab106793</a>) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 5:

Testis (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 44 kDa

Observed band size: 100 kDa,46 kDa,75 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BMAL1.

All lanes:

Western blot - Anti-BMAL1 antibody (<a href='/en-us/products/primary-antibodies/bmal1-antibody-ab3350'>ab3350</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 68 kDa

Observed band size: 51 kDa,75 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab115262 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-TBX18 antibody (<a href='/en-us/products/primary-antibodies/tbx18-antibody-ab115262'>ab115262</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg

Lane 4:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 64 kDa

Observed band size: 68 kDa,70 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to TSLP.

All lanes:

Western blot - Anti-TSLP antibody (<a href='/en-us/products/primary-antibodies/tslp-antibody-ab47943'>ab47943</a>) at 1 µg/mL

All lanes:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 18 kDa

Observed band size: 28 kDa,48 kDa,51 kDa,53 kDa

true

Exposure time: 4min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

Secondary antibody - anti-rabbit HRP (IgG H&L; ab97080)

All lanes:

Western blot - Anti-Chk2 (phospho T68) antibody (<a href='/en-us/products/primary-antibodies/chk2-phospho-t68-antibody-ab85743'>ab85743</a>) at 1 µg/mL

Lane 1:

HeLa Whole Cell Lysate - Irradiated (5Gy) at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa Whole Cell Lysate - Irradiated (5Gy) at 10 µg with Human Chk2 (phospho T68) peptide (<a href='/en-us/products/unavailable/human-chk2-phospho-t68-peptide-ab111650'>ab111650</a>)

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg with Human Chk2 (phospho T68) peptide (<a href='/en-us/products/unavailable/human-chk2-phospho-t68-peptide-ab111650'>ab111650</a>)

Lane 5:

HeLa Whole Cell Lysate - Irradiated (5Gy) at 10 µg with Human Chk2 peptide (<a href='/en-us/products/unavailable/human-chk2-peptide-ab118179'>ab118179</a>)

Lane 6:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg with Human Chk2 peptide (<a href='/en-us/products/unavailable/human-chk2-peptide-ab118179'>ab118179</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 61 kDa

Observed band size: 145 kDa,38 kDa,70 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-Cyclin D3/CCND3 antibody (<a href='/en-us/products/primary-antibodies/cyclin-d3-ccnd3-antibody-ab112034'>ab112034</a>) at 1 µg/mL

All lanes:

Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa,56 kDa

true

Exposure time: 16min

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of MEIS1. The predicted molecular weights of isoforms 1 and 2 are 43 kDa and 50 kDa respectively. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-MEIS1 antibody (<a href='/en-us/products/primary-antibodies/meis1-antibody-ab19867'>ab19867</a>) at 1 µg/mL

All lanes:

KG-1 (Human myeloid cell line) Nuclear Lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 40 kDa,48 kDa,58 kDa

false

Exposure time: 12min

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Aromatase antibody (<a href='/en-us/products/primary-antibodies/aromatase-antibody-ab35604'>ab35604</a>) at 1 µg/mL

All lanes:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 55 kDa

true

Exposure time: 150s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Transferrin Receptor antibody (<a href='/en-us/products/primary-antibodies/transferrin-receptor-antibody-ab84036'>ab84036</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate at 10 µg

Lane 3:

U-2 OS (Human osteosarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 84 kDa

Observed band size: 37 kDa,98 kDa

true

Exposure time: 4min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The mature myeloperoxidase protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). Our immunogen sequence is within the myeloperoxidase light chain. In HL60 cells, ab45977 detects bands at approximately 84-kDa, corresponding to the expected MW of full-length Myeloperoxidase protein, and at 12-kDa, corresponding to the expected MW of myeloperoxidase light chain.

All lanes:

Western blot - Anti-Myeloperoxidase antibody (<a href='/en-us/products/primary-antibodies/myeloperoxidase-antibody-ab45977'>ab45977</a>) at 1 µg/mL

All lanes:

HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 83 kDa

Observed band size: 12 kDa,35 kDa,75 kDa,84 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

Primary ab9695 was used at a dilution of 0.2 ug/ml.

Lane 1 : 250 ng Recombinant human EGF protein

Lane 2 : 125 ng Recombinant human EGF protein

All lanes:

Western blot - Anti-EGF antibody (<a href='/en-us/products/primary-antibodies/egf-antibody-ab9695'>ab9695</a>) at 0.2 µg/mL

Lane 1:

Recombinant Human EGF protein (<a href='/en-us/products/unavailable/recombinant-human-egf-protein-ab55483'>ab55483</a>) at 0.1 µg

Lane 2:

Recombinant Human EGF protein (<a href='/en-us/products/unavailable/recombinant-human-egf-protein-ab55483'>ab55483</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 133 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-RAB7 antibody (<a href='/en-us/products/primary-antibodies/rab7-antibody-ab77993'>ab77993</a>) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Observed band size: 23 kDa,34 kDa,49 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

CD40 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of CD40 is 31 kDa (SwissProt), however we expect to observe a banding pattern around 43 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-CD40 antibody (<a href='/en-us/products/primary-antibodies/cd40-antibody-ab113701'>ab113701</a>) at 1 µg/mL

Lane 1:

Human spleen tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 10 µg

Lane 2:

Human thymus tissue lysate - total protein (<a href='/en-us/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 30 kDa

Observed band size: 105 kDa,41 kDa,43 kDa

true

Exposure time: 10min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

ab69630 recognizes the partial tagged recombinant Human galectin 9 / Gal-9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.

All lanes:

Western blot - Anti-galectin 9/Gal-9 antibody (<a href='/en-us/products/primary-antibodies/galectin-9-gal-9-antibody-ab69630'>ab69630</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant Human galectin 9/Gal-9 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-galectin-9-gal-9-protein-ab93649'>ab93649</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 40 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-TCF3 / E2A (phospho T355) antibody (<a href='/en-us/products/primary-antibodies/tcf3-e2a-phospho-t355-antibody-ab85772'>ab85772</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg with Human TCF3 / E2A peptide (<a href='/en-us/products/unavailable/human-tcf3-e2a-peptide-ab118180'>ab118180</a>)

Lane 4:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg with Human TCF3 / E2A peptide (<a href='/en-us/products/unavailable/human-tcf3-e2a-peptide-ab118180'>ab118180</a>)

Lane 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg with Human TCF3 / E2A (phospho T355) peptide (<a href='/en-us/products/unavailable/human-tcf3-e2a-phospho-t355-peptide-ab103576'>ab103576</a>)

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg with Human TCF3 / E2A (phospho T355) peptide (<a href='/en-us/products/unavailable/human-tcf3-e2a-phospho-t355-peptide-ab103576'>ab103576</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 67 kDa

Observed band size: 40 kDa,80 kDa

true

Exposure time: 20min

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-delta 1 Catenin/CAS antibody (<a href='/en-us/products/primary-antibodies/delta-1-catenin-cas-antibody-ab71138'>ab71138</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 20 µg

Lane 3:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa,50 kDa,56 kDa,68 kDa,73 kDa

true

Exposure time: 3min

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-Drosha antibody (<a href='/en-us/products/primary-antibodies/drosha-antibody-ab12286'>ab12286</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 159 kDa

Observed band size: 115 kDa,170 kDa

true

Exposure time: 12min

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (<a href='/en-us/products/primary-antibodies/nnos-neuronal-phospho-s847-antibody-ab16650'>ab16650</a>) at 1 µg/mL

Lane 1:

Forebrain (Mouse) Tissue Lysate at 20 µg

Lane 2:

Spinal Cord (Mouse) Tissue Lysate at 20 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 160 kDa

Observed band size: 190 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

Secondary antibody - goat anti-rabbit HRP (H&L preadsorbed; ab97080)

All lanes:

Western blot - Anti-EpCAM antibody (<a href='/en-us/products/primary-antibodies/epcam-antibody-ab71916'>ab71916</a>) at 1 µg/mL

Lane 1:

Colon (Mouse) Tissue Lysate at 10 µg

Lane 2:

Colon (Rat) Tissue Lysate at 10 µg

Lane 3:

HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg

Lane 4:

SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

HuES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg

Lane 7:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa,40 kDa

true

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

In the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.

All lanes:

Western blot - Anti-Zhangfei antibody (<a href='/en-us/products/primary-antibodies/zhangfei-antibody-ab28700'>ab28700</a>) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 20 µg

Lane 2:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 20 µg

Lane 3:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 20 µg

Lane 4:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 20 µg

Lane 5:

293T Embryonic Kidney cell line (Human) Whole Cell Lysate at 20 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 7:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Lane 8:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 9:

PC-3 whole cell lysate (<a href='/en-us/products/unavailable/pc-3-whole-cell-lysate-ab3954'>ab3954</a>) at 20 µg

Lane 10:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 37 kDa

Observed band size: 50 kDa

false

Exposure time: 2min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

The predicted molecular weight of GOLPH3/MIDAS is 34-kDa. We are unsure why we are seeing an additional band in HeLa and A549 whole cell lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-GOLPH3/MIDAS antibody (<a href='/en-us/products/primary-antibodies/golph3-midas-antibody-ab98023'>ab98023</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Lung (Human) Tissue Lysate at 10 µg

Lane 3:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - Mouse lung normal tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-lung-normal-tissue-lysate-total-protein-ab29297'>ab29297</a>) at 10 µg

Lane 5:

Lung (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 34 kDa

Observed band size: 32 kDa,34 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

KLB contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-KLB antibody (<a href='/en-us/products/primary-antibodies/klb-antibody-ab106794'>ab106794</a>) at 1 µg/mL

All lanes:

HepG2 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 119 kDa

Observed band size: 125 kDa,39 kDa,54 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080)

All lanes:

Western blot - Anti-XAF1 antibody (<a href='/en-us/products/primary-antibodies/xaf1-antibody-ab110729'>ab110729</a>) at 1 µg/mL

All lanes:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 35 kDa

Observed band size: 37 kDa

true

Exposure time: 1min