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AB72465

Goat Anti-Rabbit IgG H&L (PE) preadsorbed

5

(1 Review)

|

(18 Publications)

Goat Anti-Rabbit IgG H&L (PE) is a pre-adsorbed secondary antibody with a maximum absorption wavelength of 480;565nm and a maximum emission wavelength of 578nm. Suitable for Flow Cytometry and ELISA

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 10 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images
Flow Cytometry - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)
  • Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.

Sandwich ELISA - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)
  • sELISA

Unknown

Sandwich ELISA - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Cow

Pre-adsorbed

Yes

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

Flow Cyt, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/1000", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 1.71% Sucrose, 1.09% disodium hydrogen phosphate, 0.87% Sodium chloride, 0.27% sodium dihydrogen phosphate, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:910 PubMed38098048

2023

Comparison of retinal degeneration treatment with four types of different mesenchymal stem cells, human induced pluripotent stem cells and RPE cells in a rat retinal degeneration model.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Jun Liu,Minmei Guo,Tzu-Cheng Sung,Ting Wang,Tao Yu,Zeyu Tian,Guoping Fan,Wencan Wu,Akon Higuchi

PeerJ 11:e15894 PubMed37727693

2023

Tibial cortex transverse transport potentiates diabetic wound healing activation of SDF-1/CXCR4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shuanji Ou,Xiaodong Wu,Yang Yang,Changliang Xia,Wei Zhang,Yudun Qu,Jiaxuan Li,Bo Chen,Lilin Zhu,Changpeng Xu,Yong Qi

Nature immunology 23:1763-1776 PubMed36316474

2022

Nuclear corepressors NCOR1/NCOR2 regulate B cell development, maintain genomic integrity and prevent transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Robin D Lee,Todd P Knutson,Sarah A Munro,Jeffrey T Miller,Lynn M Heltemes-Harris,Charles G Mullighan,Kristen Jepsen,Michael A Farrar

Frontiers in endocrinology 13:997745 PubMed36187089

2022

Myeloid cell-derived catecholamines influence bone turnover and regeneration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie R Kuhn,Melanie Haffner-Luntzer,Elena Kempter,Stefan O Reber,Hiroshi Ichinose,Jean Vacher,Anita Ignatius,Miriam E A Tschaffon-Müller

Frontiers in immunology 13:948658 PubMed36148244

2022

Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Panpan Qiang,Juan Hao,Fan Yang,Yutong Han,Yi Chang,Yunqian Xian,Yunzhao Xiong,Xiaomeng Gao,Lijuan Liang,Tatsuo Shimosawa,Qingyou Xu

Advanced biology 6:e2101317 PubMed35347890

2022

Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure.

Applications

Unspecified application

Species

Unspecified reactive species

Burak Derkus,Melis Isik,Cemil Can Eylem,Irem Ergin,Can Berk Camci,Sila Bilgin,Caglar Elbuken,Yavuz Emre Arslan,Merve Akkulak,Orhan Adali,Fadime Kiran,Babatunde O Okesola,Emirhan Nemutlu,Emel Emregul

Frontiers in nutrition 9:854780 PubMed35399691

2022

Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed in Tongue Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amira M Gamal-Eldeen,Bassem M Raafat,Amani A Alrehaili,Sherien M El-Daly,Nahed Hawsawi,Hamsa J Banjer,Eman M Raafat,Mazen M Almehmadi

Environmental toxicology 37:212-223 PubMed34655286

2021

Involvement of miRNAs in response to oxidative stress induced by the steroidal glycoalkaloid α-solanine in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaimaa A Gouhar,Mahmoud T Abo-Elfadl,Amira M Gamal-Eldeen,Sherien M El-Daly

Cell reports 35:109293 PubMed34192535

2021

Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning.

Applications

Unspecified application

Species

Unspecified reactive species

Adam W Watson,Adam D Grant,Sara S Parker,Samantha Hill,Michael B Whalen,Jayati Chakrabarti,Michael W Harman,Mackenzie R Roman,Brittany L Forte,Cody C Gowan,Raúl Castro-Portuguez,Lindsey K Stolze,Christian Franck,Darren A Cusanovich,Yana Zavros,Megha Padi,Casey E Romanoski,Ghassan Mouneimne

Frontiers in pharmacology 11:632809 PubMed33584313

2021

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Guo,Qiuju Ran,Chun Sun,Tingting Zhou,Xi Yang,Jizhou Zhang,Shifeng Pang,Yechen Xiao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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