AB72465

Goat Anti-Rabbit IgG H&L (PE) preadsorbed

Name: Goat Anti-Rabbit IgG (PE) preadsorbed secondary antibody (ab72465) | Abcam
Brand: Abcam Limited
SKU: AB72465
Price: 210 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(23 Publications)

Goat Anti-Rabbit IgG H&L (PE) is a pre-adsorbed secondary antibody with a maximum absorption wavelength of 480;565nm and a maximum emission wavelength of 578nm. Suitable for Flow Cytometry and ELISA

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 10 publications

2 Images

Flow Cytometry - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.

Sandwich ELISA - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

sELISA

Unknown

Sandwich ELISA - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Cow

Pre-adsorbed

Yes

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

Flow Cyt, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/1000", "notes":"<p></p>" } } }

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 1.71% Sucrose, 1.09% disodium hydrogen phosphate, 0.87% Sodium chloride, 0.27% sodium dihydrogen phosphate, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Protein science : a publication of the Protein Society 34:e70263 PubMed40815278

2025

Computational modeling and experimental validation of the interaction between tumor biomarker mesothelin and an engineered targeting protein with therapeutic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Piccardi,Valeria Butera,Ignazio Sardo,Stefano Landi,Federica Gemignani,Giampaolo Barone,Angelo Spinello,Sarah J Moore

Regenerative biomaterials 12:rbaf035 PubMed40416648

2025

Differentiation of human induced pluripotent stem cells into retinal pigment epithelium cells during culture on peptide-grafted hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,Qian Liu,Minmei Guo,Chengyu Jiang,Jianyang Chen,Ting Wang,Tzu-Cheng Sung,Shih-Jie Chou,Shih-Hwa Chiou,Guoping Fan,Akon Higuchi

Viruses 17: PubMed40006897

2025

SARS-CoV-2 Impairs Osteoblast Differentiation Through Spike Glycoprotein and Cytokine Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Nicole Freiberger,Cynthia Alicia Marcela López,Patricio Jarmoluk,María Belén Palma,Cintia Cevallos,Franco Agustin Sviercz,Tomás Martín Grosso,Marcela Nilda García,Jorge Quarleri,M Victoria Delpino

Cell cycle (Georgetown, Tex.) 22:2584-2601 PubMed38197217

2024

Translocated HMGB3 is involved in papillary thyroid cancer progression by activating cytoplasmic TLR3 and transmembrane TREM1.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Hong-Jun Lv,Xue-Yang Deng,Pu Chen,Malgorzata A Garstka,Bing-Yin Shi,Jiao Fu

Journal of translational medicine 21:910 PubMed38098048

2023

Comparison of retinal degeneration treatment with four types of different mesenchymal stem cells, human induced pluripotent stem cells and RPE cells in a rat retinal degeneration model.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Jun Liu,Minmei Guo,Tzu-Cheng Sung,Ting Wang,Tao Yu,Zeyu Tian,Guoping Fan,Wencan Wu,Akon Higuchi

PeerJ 11:e15894 PubMed37727693

2023

Tibial cortex transverse transport potentiates diabetic wound healing activation of SDF-1/CXCR4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shuanji Ou,Xiaodong Wu,Yang Yang,Changliang Xia,Wei Zhang,Yudun Qu,Jiaxuan Li,Bo Chen,Lilin Zhu,Changpeng Xu,Yong Qi

CNS neuroscience & therapeutics 30:e14431 PubMed37697956

2023

CD8 T cell infiltration and proliferation in the brainstem during experimental cerebral malaria.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Qinghao Zhu,Yan Shen,Jiao Liang,Yi Wang,Yuxiao Huang,Guodong Tong,Xu Wang,Ningning Zhang,Kangjie Yu,Yinghui Li,Ya Zhao

Nature immunology 23:1763-1776 PubMed36316474

2022

Nuclear corepressors NCOR1/NCOR2 regulate B cell development, maintain genomic integrity and prevent transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Robin D Lee,Todd P Knutson,Sarah A Munro,Jeffrey T Miller,Lynn M Heltemes-Harris,Charles G Mullighan,Kristen Jepsen,Michael A Farrar

Frontiers in endocrinology 13:997745 PubMed36187089

2022

Myeloid cell-derived catecholamines influence bone turnover and regeneration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie R Kuhn,Melanie Haffner-Luntzer,Elena Kempter,Stefan O Reber,Hiroshi Ichinose,Jean Vacher,Anita Ignatius,Miriam E A Tschaffon-Müller

Frontiers in immunology 13:948658 PubMed36148244

2022

Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Panpan Qiang,Juan Hao,Fan Yang,Yutong Han,Yi Chang,Yunqian Xian,Yunzhao Xiong,Xiaomeng Gao,Lijuan Liang,Tatsuo Shimosawa,Qingyou Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (PE) preadsorbed

Flow Cytometry - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

Sandwich ELISA - Goat Anti-Rabbit IgG H&L (PE) preadsorbed (AB72465)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (23)

Product promise