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AB7085

Goat Anti-Rabbit IgG H&L preadsorbed

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(5 Publications)

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF, SDS-PAGE. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images
Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)

Western blot analysis of ab7085. Secondary antibody : Anti-Goat IgG (Donkey) Peroxidase Conjugated Antibody at 1/40000 dilution for 30 min at RT.

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (ab7085) at 1/1000 dilution

Lane 1:

Rabbit IgG at 50 ng

Lane 2:

Rabbit IgG F(ab) Fragment at 50 ng

Lane 3:

Rabbit IgG F(c) Fragment at 50 ng

Lane 4:

Rabbit IgM Whole Molecule at 50 ng

Lane 5:

Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (Goat) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. at 50 ng

Secondary

All lanes:

Anti-Goat IgG at 1/40000 dilution

false

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)

Western blot analysis of ab7085. Secondary antibody : DyLight™ 800 Goat secondary antibody 1/20000 dilution for 30 min at RT.

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L preadsorbed (ab7085) at 1/1000 dilution

All lanes:

Rabbit IgG at 100 ng

Secondary

All lanes:

DyLight™ 800 goat secondary antibody 1:20,000 for 30 min at 1/20000 dilution

false

SDS-PAGE - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Goat Anti-Rabbit IgG H&L preadsorbed (AB7085)

SDS-PAGE analysis of ab7085.

Lane 1 : Nonreduced Rabbit IgG whole molecule.

Lane 2 : Reduced Rabbit IgG whole molecule.

4-20% SDS Gel, Coomassie Blue Stained.

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Applications

Conjugation, IHC-Fr, SDS-PAGE, IP, ICC/IF, ID, WB, IHC-P, ELISA

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA against at less than 1% of target signal.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2000", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/270000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2000", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e15872 PubMed37223716

2023

Efficacy of puerarin in rats with focal cerebral ischemia through modulation of the SIRT1/HIF-1α/VEGF signaling pathway and its effect on synaptic plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liu,Xiwen Sui,Yuqin Zhang,Rongchao Yue,Shifu Yin

RSC advances 12:31711-31728 PubMed36380932

2022

Nanotechnology meets immunology towards a rapid diagnosis solution: the COVID-19 outbreak challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Santos B S A S,Cunha J L R,Carvalho I C,Costa J M C,Longo B C,Galinari G C F,Diniz P H S M,Mendes G M M,Fonseca F G,Abrahão J S,Mansur A A P,Leite M F,Oréfice R L,Lobato Z I P,Mansur H S

Disease markers 2022:4607966 PubMed36267458

2022

Ferulic Acid Mitigates Growth and Invasion of Esophageal Squamous Cell Carcinoma through Inducing Ferroptotic Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Cao,Hong Zhang,Jianming Tang,Rui Wang

Oncology reports 40:241-251 PubMed29749472

2018

Insufficient radiofrequency ablation promotes hepatocellular carcinoma cell progression via autophagy and the CD133 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofei Wang,Qingsong Deng,Kai Feng,Shihan Chen,Jiayun Jiang,Feng Xia,Kuansheng Ma,Ping Bie

Molecular medicine reports 12:6738-44 PubMed26328522

2015

STAT3‑regulated long non‑coding RNAs lnc‑7SK and lnc‑IGF2‑AS promote hepatitis C virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Xiong,Ming Jia,Jing Yuan,Changjiang Zhang,Yan Zhu,Xuemei Kuang,Lin Lan,Xiaohong Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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