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AB150095

Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647)

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(2 Publications)

Suitable for IHC-Fr, ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 2 publications.

View Alternative Names

Ig mu chain C region secreted form

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647) (AB150095)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647) (AB150095)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rabbit normal spleen labelled for Rabbit IgM mu chain using ab150095.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using retrieval settings of 100°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab150095 at 1/2000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647) (AB150095)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647) (AB150095)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rabbit normal spleen labelled for Rabbit IgM mu chain using ab150095.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using retrieval settings of 100°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab150095 at 1/2000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Alexa Fluor® - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647) (AB150095)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgM mu chain (Alexa Fluor® 647) (AB150095)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgM

Target specificity

Mu chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

IHC-Fr, Flow Cyt, ELISA, IHC-P, ICC/IF

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 31:1654-1662 PubMed32293985

2020

Matrix stiffness-sensitive long noncoding RNA NEAT1 seeded paraspeckles in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vanja Todorovski,Archa H Fox,Yu Suk Choi

The Annals of thoracic surgery 99:612-8 PubMed25499473

2014

Development of decellularized aortic valvular conduit coated by heparin-SDF-1α multilayer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxin Zhou,Xiaofeng Ye,Zhe Wang,Jun Liu,Busheng Zhang,Jiapei Qiu,Yanjun Sun,Haiqing Li,Qiang Zhao
View all publications
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Product promise

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