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AB96972

Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed

5

(1 Review)

|

(0 Publication)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Ig gamma-1 chain C region

2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (AB96972)
  • ICC/IF

AbReview40940****

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (AB96972)

Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.

This image is courtesy of an Abreview submitted by Ya Zhou

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (AB96972)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (AB96972)

Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972) was used as a secondary antibody at a concentration of 1/500.

This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }
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Product details

DyLight® 550 replaces DyLight® 549.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Ig gamma-1 chain C region
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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