Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining.
Images
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Mouse, Human
- Conjugation
- DyLight® 550
- Applications
- ICC/IF, Flow Cyt, IHC-P
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining.
Key facts
- Description
- Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Mouse, Human
- Conjugation
- DyLight® 550
- Applications
- ICC/IF, Flow Cyt, IHC-P
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
DyLight® 550 replaces DyLight® 549.
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2 product images
This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)
Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972)
was used as a secondary antibody at a concentration of 1/500.
This image is courtesy of a customer review submitted by Ya ZhouImmunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)
Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.
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