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AB96974

Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed

5

(1 Review)

|

(0 Publication)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Ig gamma-1 chain C region

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (AB96974)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (AB96974)

Anti-dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974) was used as a secondary antibody at a concentration of 1/500.

This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (AB96974)
  • ICC/IF

AbReview

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (AB96974)

Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells permeabilized with 0.2% Triton X-100 using ab96974 (red) at 1/500 in PBS + 2% BSA for 1 hour.

This image is courtesy of an Abreview submitted by Ya Zhou

  • Unconjugated

    Goat Anti-Rat IgG Fc preadsorbed

  • Biotin

    Goat Anti-Rat IgG Fc (Biotin) preadsorbed

  • 576 DyLight® 550

    Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed

  • 519 FITC

    Goat Anti-Rat IgG Fc (FITC) preadsorbed

  • HRP

    Goat Anti-Rat IgG Fc (HRP) preadsorbed

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }
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Product details

DyLight® 650 replaces DyLight® 649.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information
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