Goat Anti-Rat IgG Fc (HRP) preadsorbed

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cytokeratin-19-antibody-ep1580y-rat-igg2a-chimeric-ab323561'>ab323561</a>) at 1/1000 dilution

Lane 1:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human stomach tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090) at 1/20000 dilution

Observed band size: 44 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : SH-SY5Y (PMID : 30650643 and PMID : 27345400). Spleen (PMID : 24309898).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 4 seconds; Lanes 3-4 : 37 seconds

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cytokeratin-19-antibody-ep1580y-rat-igg2a-chimeric-ab323561'>ab323561</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

Human colon tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : 17893876, 11454801). Exposure times : Lanes 1-5 : 15 seconds Lanes 6-7 : 81 seconds

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/poliovirus-receptor-pvr-antibody-epr22672-151-rat-igg1-chimeric-ab307687'>ab307687</a>) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 20 μg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

Observed band size: 70 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Blocking / Diluting buffer and concentration : 5% NFDM/TBST In Western blot, ab305107 was shown to bind specifically to ROCK1. A band was observed at 158 kDa in wild-type HAP1 cell lysates with whereas no signal observed at this size in ROCK1 knockout cell lysate. Exposure time : 3 minutes.

All lanes:

Western blot - Anti-ROCK1 antibody [EPR638Y] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/rock1-antibody-epr638y-rat-igg2a-chimeric-ab305107'>ab305107</a>) at 1/1000 dilution

Lane 1:

Wide-type HAP1 whole cell lysate at 10 µg

Lane 2:

ROCK1 knockout HAP1 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090) at 1/20000 dilution

Observed band size: 158 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : Raji (PMID : 10915595;PMID : 9880562).                                                           PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : 17893876, 11454801). Exposure time : 26 seconds.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/poliovirus-receptor-pvr-antibody-epr22672-151-rat-igg1-chimeric-ab307687'>ab307687</a>) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

WI-38 (human fetal lung fibroblast) whole cell lysate 20 μg

Lane 3:

HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate 20 μg

Lane 4:

HUVEC (human umbilical vein endothelial cell) whole cell lysate 20 μg

Lane 5:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate 20 μg

Observed band size: 70 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID : 9712057 & 16517975). Negative control : U937, THP-1 (PMID : 16368951, 10648003). Exposure time : 37 seconds

All lanes:

Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cd163-antibody-epr19518-rat-igg2a-chimeric-ab289979'>ab289979</a>) at 1/1000 dilution

Lane 1:

SU-DHL-1 (human large cell lymphoma histiocytic), whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

Lane 3:

U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Spleen (PMID : 24309898)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cytokeratin-19-antibody-ep1580y-rat-igg2a-chimeric-ab323561'>ab323561</a>) at 1/1000 dilution

Lane 1:

Mouse colon tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Rat colon tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (AB97090)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID : 9712057 & 16517975). Exposure time : Lane 1 : 81 seconds Lane 2 : 37 seconds

All lanes:

Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cd163-antibody-epr19518-rat-igg2a-chimeric-ab289979'>ab289979</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 37s