Suitable for ICC, WB, ELISA, IHC-P.
Images
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Applications
- ICC, WB, ELISA, IHC-P
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000.00000 - 1/30000.00000 | Notes (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000) |
Application ELISA | Reactivity Reacts | Dilution info 1/100.00000 - 1/30000.00000 | Notes (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000) |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ICC, WB, ELISA, IHC-P.
Key facts
- Description
- Goat Anti-Rat IgG H&L
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Applications
- ICC, WB, ELISA, IHC-P
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Goat Anti-Rat IgG H&L (ab97053)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The samples were run on a Bis-Tris gel under reducing conditions.
Western blot: Anti-WTAP (phospho S341)antibody (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
The identity of the lower MW at approximately 35KD is unknown.
To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescentwestern blot (TBS-based) blocking solution before incubation with primaryantibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.All lanes: Western blot - Anti-WTAP (phospho S341) antibody [EPR27039-57] (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) at 1/1000 dilution
All lanes: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (ab97053) at 1/100000 dilution
Observed band size: 55 kDa
Downloads
Product protocols
- Visit the general protocols
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