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AB97053

Goat Anti-Rat IgG H&L

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Suitable for ICC, WB, ELISA, IHC-P.

View Alternative Names

Ig gamma-1 chain C region

1 Images
Western blot - Goat Anti-Rat IgG H&L (AB97053)
  • WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (AB97053)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The samples were run on a Bis-Tris gel under reducing conditions. Western blot : Anti-WTAP (phospho S341)antibody (ab309350) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (ab8245) loading control staining at 1/20000 dilution, shown in red. The identity of the lower MW at approximately 35KD is unknown. To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescentwestern blot (TBS-based) blocking solution before incubation with primaryantibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

All lanes:

Western blot - Anti-WTAP (phospho S341) antibody [EPR27039-57] (<a href='/en-us/products/primary-antibodies/wtap-phospho-s341-antibody-epr27039-57-ab309350'>ab309350</a>) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (ab97053) at 1/100000 dilution

Observed band size: 55 kDa

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  • HRP polymer

    Goat Anti-Rat IgG H&L (HRP polymer)

  • 615 Texas Red®

    Goat Anti-Rat IgG H&L (Texas Red ®) preadsorbed

  • 572 TRITC

    Goat Anti-Rat IgG H&L (TRITC) preadsorbed

  • 578 PE

    Goat anti-Rat IgG H&L (PE) Pre-Adsorbed

  • 667 Cy5®

    Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rat IgG H&L (Cy2 ®) preadsorbed

  • 10nm Gold

    Goat Anti-Rat IgG H&L (10nm Gold)

  • 703 Cy5.5®

    Goat Anti-Rat IgG H&L (Cy5.5 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rat IgG H&L (FITC)

  • Alkaline Phosphatase

    Goat Anti-Rat IgG H&L (Alkaline Phosphatase)

  • 576 DyLight® 550

    Goat Anti-Rat IgG H&L (DyLight® 550)

  • 578 PE

    Goat Anti-Rat IgG H&L (PE)

  • 618 DyLight® 594

    Goat Anti-Rat IgG H&L (DyLight® 594) preadsorbed

  • 519 Alexa Fluor® 488

    Goat Anti-Rat IgG H&L (Alexa Fluor® 488)

  • 617 Alexa Fluor® 594

    Goat Anti-Rat IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rat IgG H&L (Alexa Fluor® 680)

  • 805 Alexa Fluor® 790

    Goat Anti-Rat IgG H&L (Alexa Fluor® 790)

  • 603 Alexa Fluor® 568

    Goat Anti-Rat IgG H&L (Alexa Fluor® 568)

  • Biotin

    Goat Anti-Rat IgG H&L (Biotin)

  • HRP

    Goat Anti-Rat IgG H&L (HRP)

  • 421 Alexa Fluor® 405

    Goat Anti-Rat IgG H&L (Alexa Fluor® 405)

  • 518 DyLight® 488

    Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed

  • 565 Cy3®

    Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed

  • Unconjugated

    Goat Anti-Rat IgG H&L preadsorbed

  • 673 DyLight® 650

    Goat Anti-Rat IgG H&L (DyLight® 650) preadsorbed

  • 565 Alexa Fluor® 555

    Goat Anti-Rat IgG H&L (Alexa Fluor® 555)

  • 775 Alexa Fluor® 750

    Goat Anti-Rat IgG H&L (Alexa Fluor® 750)

  • 665 Alexa Fluor® 647

    Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Applications

IHC-P, ICC, ELISA, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/30000", "notes":"<p>(Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000)</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/30000", "notes":"<p>(Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000)</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information
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