Goat Anti-Rat IgG H&L (Alexa Fluor® 405)
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(19 Publications)
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 19 publications.
View Alternative Names
Ig gamma-1 chain C region
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)
ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175671 Alexa Fluor® 405 goat anti-rat IgG (H+L) used at 1μg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)
Reactivity data
Product details
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
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Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Publications (19)
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Science advances 10:eadl4573 PubMed39392881
2024
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Bioactive materials 39:392-405 PubMed38855060
2024
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Frontiers in molecular neuroscience 17:1347540 PubMed38813436
2024
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Cell reports 42:113512 PubMed38039129
2023
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Journal of neuroscience methods 402:110012 PubMed37984591
2023
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Neuron 112:421-440.e7 PubMed37979584
2023
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Nature communications 14:3539 PubMed37322224
2023
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Methods in molecular biology (Clifton, N.J.) 2563:341-369 PubMed36227482
2022
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Molecular biology of the cell 33:ar123 PubMed36044338
2022
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Cell 184:4886-4903.e21 PubMed34433013
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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