AB175671

Goat Anti-Rat IgG H&L (Alexa Fluor® 405)

Name: Goat Anti Rat (IgG) secondary antibody Alexa Fluor® 405 (ab175671) | Abcam
Brand: Abcam Limited
SKU: AB175671
Price: 190 USD
Availability: InStock

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(19 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 19 publications.

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175671 Alexa Fluor® 405 goat anti-rat IgG (H+L) used at 1μg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)

Unconjugated

Goat Anti-Rat IgG H&L
HRP polymer

Goat Anti-Rat IgG H&L (HRP polymer)
615 Texas Red®

Goat Anti-Rat IgG H&L (Texas Red ®) preadsorbed
572 TRITC

Goat Anti-Rat IgG H&L (TRITC) preadsorbed
578 PE

Goat anti-Rat IgG H&L (PE) Pre-Adsorbed
667 Cy5®

Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
505 Cy2®

Goat Anti-Rat IgG H&L (Cy2 ®) preadsorbed
10nm Gold

Goat Anti-Rat IgG H&L (10nm Gold)
703 Cy5.5®

Goat Anti-Rat IgG H&L (Cy5.5 ®) preadsorbed
519 FITC

Goat Anti-Rat IgG H&L (FITC)
Alkaline Phosphatase

Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
576 DyLight® 550

Goat Anti-Rat IgG H&L (DyLight® 550)
578 PE

Goat Anti-Rat IgG H&L (PE)
618 DyLight® 594

Goat Anti-Rat IgG H&L (DyLight® 594) preadsorbed
519 Alexa Fluor® 488

Goat Anti-Rat IgG H&L (Alexa Fluor® 488)
617 Alexa Fluor® 594

Goat Anti-Rat IgG H&L (Alexa Fluor® 594)
702 Alexa Fluor® 680

Goat Anti-Rat IgG H&L (Alexa Fluor® 680)
805 Alexa Fluor® 790

Goat Anti-Rat IgG H&L (Alexa Fluor® 790)
603 Alexa Fluor® 568

Goat Anti-Rat IgG H&L (Alexa Fluor® 568)
Biotin

Goat Anti-Rat IgG H&L (Biotin)
HRP

Goat Anti-Rat IgG H&L (HRP)
518 DyLight® 488

Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
565 Cy3®

Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed
Unconjugated

Goat Anti-Rat IgG H&L preadsorbed
673 DyLight® 650

Goat Anti-Rat IgG H&L (DyLight® 650) preadsorbed
565 Alexa Fluor® 555

Goat Anti-Rat IgG H&L (Alexa Fluor® 555)
775 Alexa Fluor® 750

Goat Anti-Rat IgG H&L (Alexa Fluor® 750)
665 Alexa Fluor® 647

Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

IHC-Fr, IHC-P, ELISA, ICC/IF, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" } } }

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Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadl4573 PubMed39392881

2024

Shank3 mutation impairs glutamate signaling and myelination in ASD mouse model and human iPSC-derived OPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Inbar Fischer,Sophie Shohat,Yael Leichtmann-Bardoogo,Ritu Nayak,Gal Wiener,Idan Rosh,Aviram Shemen,Utkarsh Tripathi,May Rokach,Ela Bar,Yara Hussein,Ana Carolina Castro,Gal Chen,Adi Soffer,Sari Schokoroy-Trangle,Galit Elad-Sfadia,Yaniv Assaf,Avi Schroeder,Patricia Monteiro,Shani Stern,Ben M Maoz,Boaz Barak

Bioactive materials 39:392-405 PubMed38855060

2024

C176-loaded and phosphatidylserine-modified nanoparticles treat retinal neovascularization by promoting M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

An Shao,Lulu Jin,Yanni Ge,Ziqiang Ye,Mingyu Xu,Yifan Zhou,Yingyu Li,Linyan Wang,Pinglong Xu,Kai Jin,Zhengwei Mao,Juan Ye

Frontiers in molecular neuroscience 17:1347540 PubMed38813436

2024

Horizontal-cell like Dm9 neurons in modulate photoreceptor output to supply multiple functions in early visual processing.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Schnaitmann,Manuel Pagni,Patrik B Meyer,Lisa Steinhoff,Vitus Oberhauser,Dierk F Reiff

Cell reports 42:113512 PubMed38039129

2023

Leptin signaling in the dorsomedial hypothalamus couples breathing and metabolism in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Mateus R Amorim,Xin Wang,O Aung,Shannon Bevans-Fonti,Frederick Anokye-Danso,Caitlin Ribeiro,Joan Escobar,Carla Freire,Huy Pho,Olga Dergacheva,Luiz G S Branco,Rexford S Ahima,David Mendelowitz,Vsevolod Y Polotsky

Journal of neuroscience methods 402:110012 PubMed37984591

2023

Development of a monoclonal antibody specific for a calpain-generated ∆48 kDa calcineurin fragment, a marker of distressed astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Susan D Kraner,Pradoldej Sompol,Siriyagon Prateeptrang,Moltira Promkan,Suthida Hongthong,Napasorn Thongsopha,Peter T Nelson,Christopher M Norris

Neuron 112:421-440.e7 PubMed37979584

2023

Reorganization of adolescent prefrontal cortex circuitry is required for mouse cognitive maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Jastyn A Pöpplau,Timo Schwarze,Mariia Dorofeikova,Irina Pochinok,Anne Günther,Annette Marquardt,Ileana L Hanganu-Opatz

Nature communications 14:3539 PubMed37322224

2023

Combinatorial encoding of odors in the mosquito antennal lobe.

Applications

Unspecified application

Species

Unspecified reactive species

Pranjul Singh,Shefali Goyal,Smith Gupta,Sanket Garg,Abhinav Tiwari,Varad Rajput,Alexander Shakeel Bates,Arjit Kant Gupta,Nitin Gupta

Methods in molecular biology (Clifton, N.J.) 2563:341-369 PubMed36227482

2022

Synthetic Organelles for Multiple mRNA Selective Genetic Code Expansions in Eukaryotes.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher D Reinkemeier,Edward A Lemke

Molecular biology of the cell 33:ar123 PubMed36044338

2022

Spatiotemporal analysis of axonal autophagosome-lysosome dynamics reveals limited fusion events and slow maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney E Cason,Saurabh S Mogre,Erika L F Holzbaur,Elena F Koslover

Cell 184:4886-4903.e21 PubMed34433013

2021

Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher D Reinkemeier,Edward A Lemke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rat IgG H&L (Alexa Fluor® 405)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) (AB175671)

Related conjugates and formulations (28)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (19)

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