AB175673

Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed

Name: Goat Anti-Rat IgG (Alexa Fluor® 405) preadsorbed secondary antibody (ab175673) | Abcam
Brand: Abcam Limited
SKU: AB175673
Price: 285 USD
Availability: InStock

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(6 Publications)

Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175673)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175673)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175673 Alexa Fluor® 405 Goat polyclonal Secondary Antibody to Rat IgG - H&L pre-adsorbed used at 1μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175673)

Alexa Fluor®

Supplier Data

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175673)

Emission spectra of Alexa Fluor^® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ELISA, ICC/IF, IHC-P, Flow Cyt

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chain common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 19:e1010973 PubMed37747929

2023

TDP-43 pathology in Drosophila induces glial-cell type specific toxicity that can be ameliorated by knock-down of SF2/SRSF1.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Krupp,Isabel Hubbard,Oliver Tam,Gale M Hammell,Josh Dubnau

eLife 12: PubMed37309249

2023

Belly roll, a GPI-anchored Ly6 protein, regulates escape behaviors by modulating the excitability of nociceptive peptidergic interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Li,Yuma Tsukasa,Misato Kurio,Kaho Maeta,Akimitsu Tsumadori,Shumpei Baba,Risa Nishimura,Akira Murakami,Koun Onodera,Takako Morimoto,Tadashi Uemura,Tadao Usui

Molecular cell 82:3255-3269.e8 PubMed35987199

2022

Increasing cell size remodels the proteome and promotes senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Michael C Lanz,Evgeny Zatulovskiy,Matthew P Swaffer,Lichao Zhang,Ilayda Ilerten,Shuyuan Zhang,Dong Shin You,Georgi Marinov,Patrick McAlpine,Joshua E Elias,Jan M Skotheim

Cell 184:4886-4903.e21 PubMed34433013

2021

Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher D Reinkemeier,Edward A Lemke

Development (Cambridge, England) 148: PubMed33376126

2021

The enteric nervous system in zebrafish larvae can regenerate via migration into the ablated area and proliferation of neural crest-derived cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Ohno,Masataka Nikaido,Natsumi Horiuchi,Koichi Kawakami,Kohei Hatta

Neuron 97:538-554.e5 PubMed29395908

2018

Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Enriquez,Laura Quintana Rio,Richard Blazeski,Stephanie Bellemin,Pierre Godement,Carol Mason,Richard S Mann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175673)

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175673)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise