Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
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Publications
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- PLoS genetics 19:e10109732023TDP-43 pathology in Drosophila induces glial-cell type specific toxicity that can be ameliorated by knock-down of SF2/SRSF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Krupp,Isabel Hubbard,Oliver Tam,Gale M Hammell,Josh DubnauPubMed 37747929
- eLife 12:2023Belly roll, a GPI-anchored Ly6 protein, regulates escape behaviors by modulating the excitability of nociceptive peptidergic interneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Li,Yuma Tsukasa,Misato Kurio,Kaho Maeta,Akimitsu Tsumadori,Shumpei Baba,Risa Nishimura,Akira Murakami,Koun Onodera,Takako Morimoto,Tadashi Uemura,Tadao UsuiPubMed 37309249
- Molecular cell 82:3255-3269.e82022Increasing cell size remodels the proteome and promotes senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael C Lanz,Evgeny Zatulovskiy,Matthew P Swaffer,Lichao Zhang,Ilayda Ilerten,Shuyuan Zhang,Dong Shin You,Georgi Marinov,Patrick McAlpine,Joshua E Elias,Jan M SkotheimPubMed 35987199
- Cell 184:4886-4903.e212021Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher D Reinkemeier,Edward A LemkePubMed 34433013
- Development (Cambridge, England) 148:2021The enteric nervous system in zebrafish larvae can regenerate via migration into the ablated area and proliferation of neural crest-derived cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Ohno,Masataka Nikaido,Natsumi Horiuchi,Koichi Kawakami,Kohei HattaPubMed 33376126
- Neuron 97:538-554.e52018Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Enriquez,Laura Quintana Rio,Richard Blazeski,Stephanie Bellemin,Pierre Godement,Carol Mason,Richard S MannPubMed 29395908
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Sheep, Rabbit, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Applications
- ELISA, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
Key facts
- Description
- Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Sheep, Rabbit, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Applications
- ELISA, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chain common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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1 product image
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175673)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175673 Alexa Fluor® 405 Goat polyclonal Secondary Antibody to Rat IgG - H&L pre-adsorbed used at 1μg/ml for 1h. DRAQ5™ (DRAQ5™ ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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