Goat Anti-Rat IgG H&L (Alexa Fluor® 488)

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Immunohistochemistry (Frozen sections) - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling CD8 alpha with ab308264 at 100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-CD8 alpha (ab308264, green) and anti-CD4 (ab225310, red) on mouse spleen.Panel B : anti-CD8 alpha stained on the mouse spleen.Panel C : anti-CD4 stained on the mouse spleen.The section was incubated in two rounds of staining : in the order of ab308264 and ab225310 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). antibody control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488)at 1000 2 ug/mL dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Histone H3 with ab309551 at 1/50 (20.52 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells with ab10543 at 1/8000 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at a 1/1000 dilution (Magenta).

Confocal image showing nuclear staining in HeLa cells (shown in green) at metaphase, the signal is decreased after λ Protein phosphatase treatment at 30℃ for 2 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Cytokeration 19 with ab323561 at 1/4000 ( 0.2495 ug/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in MCF7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : SH-SY5Y.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/200 10ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling CEACAM1 + CEACAM3 + CEACAM6 with ab104450 at 1/250 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) used at a 1/1000 dilution (red).

Confocal image showing membranous staining in HepG2 cell line.
Negative control : HeLa (PMID : 11580753).

Nuclear DNA was labelled with DAPI (shown in blue).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CBX1 KO HAP1(human chronic myelogenous leukemia cell)/Wild-type HAP1 cells labelling CBX1 / HP1 beta with ab10811 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution(Green).

Confocal image showing nuclear staining in parental HAP1 cell line (shown in green) and negative staining in CBX1 KO HAP1 cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling xCT with ab300667 at 1/50 (21.18 ug/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membraneous and cytoplasmic staining in A549 cell line.Low expression : colo 205 (PMID : 31000598). is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling Ubiquitin (phospho S65) with ab320096 at 1/50 (18.68 ug/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing increased staining in PC-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195884 Anti-Tubulin rat monoclonal antibody - Microtubule Marker (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling EGFR with ab231 at 1/50 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) used at a 1/1000 dilution (red).

Confocal image showing strong membranous and weak cytoplasmic staining in A431 cells.
Low expression control : HEK-293 (PMID : 26368334).

Nuclear DNA was labelled with DAPI (shown in blue).

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Immunohistochemistry (Frozen sections) - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized fresh frozen mouse cerebrum tissue labeling GFAP with ab279291 at a 1/200 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) (ab150157) secondary antibody at a 1/1000 dilution. The nuclear counterstain was DAPI (blue).

Positive staining on mouse cerebrum.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) ab150157 at a 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

RAW 264.7 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab53444 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab53444 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing cytoplasmic staining in RAW 264.7 cell line.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Confocal image showing membranous staining in RAW 264.7 cells. RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab8878 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Negative control : WEHI-231 (PMID : 2457584)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling Myelin Basic Protein with ab7349 at a 1/200 dilution, followed by Goat anti-Rat (Alexa Fluor^® 488) (ab150157) secondary antibody at a 1/1000 dilution. Confocal image showing positive staining in mouse primary oligodendroglia cells (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Counterstained with Anti-MAP2 antibody (ab183830) at a 1/1000 dilution, followed by Goat Anti-Rabbit secondary (Alexa Fluor^® 594) (ab150080) at a 1/1000 dilution (shown in magenta).

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The negative controls are as follows :

-ve control 1 : Myelin Basic Protein (ab7349) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor^® 594) (ab150080) at a 1/1000 dilution.

-ve control 2 : Anti-MAP2 (ab183830) at a 1/1000 dilution, followed by Goat anti-Rat (Alexa Fluor^® 488) (ab150157) at 1/1000 dilution.

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Immunohistochemistry (Frozen sections) - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling CD8 alpha with ab308264 at 100 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308264 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). antibody control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488)at 1000 2 ug/mL dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CBX1 / HP1 beta with ab10811 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution(Green).

Confocal image showing nuclear staining in NIH/3T3 cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells with ab117579 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used for counterstain at a 1/200 dilution (Magenta).

Confocal image showing membranous and cytoplasmic staining in subsets of mouse PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized EL4 (mouse lymphoma T lymphocyte cell) cells labelling PD1 with ab309360 at 1/100 (9.8 ug/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cell line.Negative control : L-929 (mouse connective tissue fibroblast cell)Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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Flow Cytometry - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Flow cytometric analysis of Mouse splenocyte cells labelling CD8 alpha with ab308264 at 1/1000 dilution (0.1 ug)/Right compared with a Rat monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rat IgG (Alexa Fluor® 488, ab150157) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD4 conjugated to Alexa Fluor® 647. Gated on viable cells.

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Immunohistochemistry (Frozen sections) - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized fresh frozen rat cerebrum tissue labeling GFAP with ab279291 at a 1/200 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) (ab150157) secondary antibody at a 1/1000 dilution. The nuclear counterstain was DAPI (blue).

Positive staining on rat cerebrum.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) ab150157 at a 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling non-muscle Myosin IIB/MYH10 with ab300647 at 1/50 dilution (23.04 µg/ml), followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2µg/mL) (Green). Confocal image showing cytoskeletal staining in NIH/3T3 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/mL) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab300647 at a 1/50 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Histone H3 with ab309551 at 1/50 (20.52 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Flow Cytometry - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

Overlay histogram showing Jurkat cells stained with ab30446 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30446, 0.01μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 0.01μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (green) was ab150157 Alexa Fluor® 488 goat anti-rat IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

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Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (AB150157)