AB150158

Goat Anti-Rat IgG H&L (Alexa Fluor® 555)

Name: Goat Anti-Rat IgG (Alexa Fluor® 555) secondary antibody (ab150158) | Abcam
Brand: Abcam Limited
SKU: AB150158
Price: 205 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(43 Publications)

Goat Anti-Rat IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 30 publications

View Alternative Names

Ig gamma-1 chain C region

3 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150158 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.The secondary antibody (yellow) was ab150158 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

IHC-Fr, IHC-P, ICC/IF, ELISA, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" } } }

style

left-column

Product details

This antibody reacts with the heavy and light chains of Rat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information

style

left-column

Publications (43)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112699 PubMed40520101

2025

KBG syndrome-associated protein ANKRD11 regulates SETD5 expression to modulate rRNA levels and translation.

Applications

Unspecified application

Species

Unspecified reactive species

Shoko Sashiyama,Tadashi Nakagawa,Makiko Nakagawa,Masaki Hosogane,Yasuaki Watanabe,Honoka Ashitomi,Kazusa Yamane,Norihiro Shibuya,Toshiro Moroishi,Keiko Nakayama,Toru Hosoi

Glia 73:840-856 PubMed39743758

2025

Effect of Cytoskeletal Linker Protein GAS2L1 on Oligodendrocyte and Myelin Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Zou,Yili Jin,Yuqian Yang,Liuning Zhang,Yuanyu Feng,Yu Long,ZhengTao Xu,Yuehua He,Wei Zheng,Shuming Wang,Yongxiang He,Jiong Li,Huiliang Li,Zhigang Luo,Chun Hu,Lin Xiao

Scientific reports 14:25166 PubMed39448766

2024

A primate model animal revealed the inter-species differences and similarities in the subtype specifications of the spiral ganglion neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masafumi Ueno,Marie N Shimanuki,Takanori Nishiyama,Naoki Oishi,Hiroyuki Ozawa

JID innovations : skin science from molecules to population health 5:100305 PubMed39403555

2024

A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Conneely,Jin Namkoong,Francis Allison,S Kyoko Hirata Tsutsumi,Dominic Grussu,Ryan Willis,Kyle Henderson,Paul A Campbell,Melissa Moy,Ewelina Lesniak,Joanna Wu,Robyn P Hickerson

Journal of nanobiotechnology 22:464 PubMed39095755

2024

Momordica charantia L.-derived exosome-like nanovesicles stabilize p62 expression to ameliorate doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Ye,Chen Yan,Si-Jia Bian,Xin-Ran Li,Yu Li,Kai-Xuan Wang,Yu-Hua Zhu,Liang Wang,Ying-Chao Wang,Yi-Yuan Wang,Tao-Sheng Li,Su-Hua Qi,Lan Luo

Nanoscale 16:14330-14338 PubMed39015956

2024

Iron oxide nanozymes enhanced by ascorbic acid for macrophage-based cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongchao Yi,Xiaoyue Yang,Ying Liang,Sheng Tong

Molecules (Basel, Switzerland) 29: PubMed38893499

2024

Trichostatin A Promotes Cytotoxicity of Cisplatin, as Evidenced by Enhanced Apoptosis/Cell Death Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhou,Qun Luo,Fangang Zeng,Xingkai Liu,Juanjuan Han,Liangzhen Gu,Xiao Tian,Yanyan Zhang,Yao Zhao,Fuyi Wang

iScience 27:109528 PubMed38595797

2024

Reduced chondroitin sulfate content prevents diabetic neuropathy through transforming growth factor-β signaling suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Hajime Ishiguro,Takashi Ushiki,Atsuko Honda,Yasuhiro Yoshimatsu,Riuko Ohashi,Shujiro Okuda,Asami Kawasaki,Kaori Cho,Suguru Tamura,Tatsuya Suwabe,Takayuki Katagiri,Yiwei Ling,Atsuhiko Iijima,Tadahisa Mikami,Hiroshi Kitagawa,Akiyoshi Uemura,Kazunori Sango,Masayoshi Masuko,Michihiro Igarashi,Hirohito Sone

Angiogenesis 27:397-410 PubMed38546923

2024

Vascular mimicry in zebrafish fin regeneration: how macrophages build new blood vessels.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Senk,Jennifer Fazzari,Valentin Djonov

Brain, behavior, and immunity 118:178-189 PubMed38428650

2024

The chemokine XCL1 functions as a pregnancy hormone to program offspring innate anxiety.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa J Chen,Anika Nabila,Judit Gal Toth,Heidi Stuhlmann,Miklos Toth

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rat IgG H&L (Alexa Fluor® 555)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (AB150158)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (43)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise