Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Cited in 30 publications.
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- Nature communications 15:1372024p53 suppresses MHC class II presentation by intestinal epithelium to protect against radiation-induced gastrointestinal syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianming Wang,Chun-Yuan Chang,Xue Yang,Fan Zhou,Juan Liu,Jill Bargonetti,Lanjing Zhang,Ping Xie,Zhaohui Feng,Wenwei HuPubMed 38167344
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e232902023Hypoxia-sensing VGLL4 promotes LDHA-driven lactate production to ameliorate neuronal dysfunction in a cellular model relevant to Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuyun Tian,Junjie Li,Bin Wu,Jiaojiao Wang,Qian Xiao,Na Tian,Lilin Yi,Man Luo,Zhaolun Li,Yayan Pang,Xiuyu Shi,Zhifang DongPubMed 37921465
- Chinese medicine 18:1452023Therapeutic potential of Lianhua Qingke in airway mucus hypersecretion of acute exacerbation of chronic obstructive pulmonary disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanjie Hao,Tongxing Wang,Yunlong Hou,Xiaoqi Wang,Yujie Yin,Yi Liu,Ningxin Han,Yan Ma,Zhen Li,Yaru Wei,Wei Feng,Zhenhua Jia,Hui QiPubMed 37924136
- Frontiers in cell and developmental biology 11:12704082023Transcription and chromatin regulation by TAF4b during cellular quiescence of developing prospermatogonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan A Gura,Myles A Bartholomew,Kimberly M Abt,Soňa Relovská,Kimberly A Seymour,Richard N FreimanPubMed 37900284
- Communications biology 6:7492023Identifying dysregulated immune cell subsets following volumetric muscle loss with pseudo-time trajectories.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren A Hymel,Shannon E Anderson,Thomas C Turner,William Y York,Hongmanlin Zhang,Adrian R Liversage,Hong Seo Lim,Peng Qiu,Luke J Mortensen,Young C Jang,Nick J Willett,Edward A BotchweyPubMed 37468760
- Nature communications 14:21432023In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaximilian Huber,Javier Casares-Arias,Reinhard Fässler,Daniel J Müller,Nico StrohmeyerPubMed 37059721
- ACS nano 16:16019-160372022Multifunctional Gold Nanoclusters for Effective Targeting, Near-Infrared Fluorescence Imaging, Diagnosis, and Treatment of Cancer Lymphatic Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeyang Pang,Weixiao Yan,Jie Yang,Qizhen Li,Yuan Guo,Dejian Zhou,Xingyu JiangPubMed 36130729
- Magnetic resonance in medicine 89:384-3952022Gadolinium retention in the ischemic cerebrum: Implications for pain, neuron loss, and neurological deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Xin Huang,Run-Hao Jiang,Xiao-Quan Xu,Wei Wang,Yu-Qin Sun,Lei Li,Hai-Bin Shi,Sheng LiuPubMed 36111354
- Advanced healthcare materials 11:e21028162022Targeted Delivery of DNA Topoisomerase Inhibitor SN38 to Intracranial Tumors of Glioblastoma Using Sub-5 Ultrafine Iron Oxide Nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuancheng Li,Manman Xie,Joshua B Jones,Zhaobin Zhang,Zi Wang,Tu Dang,Xinyu Wang,Malgorzata Lipowska,Hui MaoPubMed 35481625
- Frontiers in cardiovascular medicine 9:8641882022-Derived Extracellular Vesicles-Like Nanovesicles Protect Cardiomyocytes Against Radiation Injury via Attenuating DNA Damage and Mitochondria Dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Wen Cui,Cong Ye,Kai-Xuan Wang,Xu Yang,Pei-Yan Zhu,Kan Hu,Ting Lan,Lin-Yan Huang,Wan Wang,Bing Gu,Chen Yan,Ping Ma,Su-Hua Qi,Lan LuoPubMed 35509278
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200 - 1/1000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Cited in 30 publications.
Key facts
- Description
- Goat Anti-Rat IgG H&L (Alexa Fluor® 555)
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (ab150158)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150158 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (ab150158)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.The secondary antibody (yellow) was ab150158 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (ab150158)
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