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AB150166

Goat Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

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(12 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 12 publications.

View Alternative Names

Ig gamma-1 chain C region

3 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150166)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150166)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150166 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150166)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150166)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150166 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150166)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150166)

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ELISA, Flow Cyt, IHC-P, ICC/IF, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chain common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Ig gamma-1 chain C region
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed39364747

2024

The role of Imp and Syp RNA-binding proteins in precise neuronal elimination by apoptosis through the regulation of transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyue Guan,Ziyan Nie,Anne Laurençon,Mathilde Bouchet,Christophe Godin,Chérif Kabir,Aurelien Darnas,Jonathan Enriquez

Micromachines 15: PubMed38930702

2024

The Profile of Network Spontaneous Activity and Functional Organization Interplay in Hierarchically Connected Modular Neural Networks In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Yana Pigareva,Arseniy Gladkov,Vladimir Kolpakov,Victor B Kazantsev,Irina Mukhina,Alexey Pimashkin

Cell 187:831-845.e19 PubMed38301645

2024

PNMA2 forms immunogenic non-enveloped virus-like capsids associated with paraneoplastic neurological syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Xu,Simon Erlendsson,Manvendra Singh,G Aaron Holling,Matthew Regier,Iosune Ibiricu,Jenifer Einstein,Michael P Hantak,Gregory S Day,Amanda L Piquet,Tammy L Smith,Stacey L Clardy,Alexandra M Whiteley,Cédric Feschotte,John A G Briggs,Jason D Shepherd

Science advances 9:eadd6480 PubMed37595032

2023

Organoid cell fate dynamics in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Zheng,Max A Betjes,Pascal Ender,Yvonne J Goos,Guizela Huelsz-Prince,Hans Clevers,Jeroen S van Zon,Sander J Tans

Brain communications 5:fcac334 PubMed36601621

2023

Altered localization of nucleoporin 98 in primary tauopathies.

Applications

Unspecified application

Species

Unspecified reactive species

John R Dickson,Matthew P Frosch,Bradley T Hyman

Frontiers in endocrinology 13:909180 PubMed35909511

2022

Human Immunocompetent Model of Neuroendocrine Liver Metastases Recapitulates Patient-Specific Tumour Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Ewald Jan Doornebal,Nicola Harris,Antonio Riva,Ravi Jagatia,Michail Pizanias,Andreas Prachalias,Krishna Menon,Melissa Preziosi,Ane Zamalloa,Rosa Miquel,Yoh Zen,Michael Robert Orford,Simon Eaton,Nigel Heaton,John Ramage,Elena Palma,Rajaventhan Srirajaskanthan,Shilpa Chokshi

Cell reports 39:110992 PubMed35767953

2022

Post-transcriptional regulation of transcription factor codes in immature neurons drives neuronal diversity.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyue Guan,Stéphanie Bellemin,Mathilde Bouchet,Lalanti Venkatasubramanian,Camille Guillermin,Anne Laurençon,Chérif Kabir,Aurélien Darnas,Christophe Godin,Séverine Urdy,Richard S Mann,Jonathan Enriquez

Cell reports methods 2:100221 PubMed35637903

2022

All-viral tracing of monosynaptic inputs to single birthdate-defined neurons in the intact brain.

Applications

Unspecified application

Species

Unspecified reactive species

R Irene Jacobsen,Rajeevkumar R Nair,Horst A Obenhaus,Flavio Donato,Torstein Slettmoen,May-Britt Moser,Edvard I Moser

Frontiers in pharmacology 13:745020 PubMed35431971

2022

Conditioned Medium From the Stem Cells of Human Exfoliated Deciduous Teeth Ameliorates Neuropathic Pain in a Partial Sciatic Nerve Ligation Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Liu,Fumiya Kano,Noboru Hashimoto,Linze Xia,Qiao Zhou,Xingmei Feng,Hideharu Hibi,Aya Miyazaki,Tsutomu Iwamoto,Yoshizo Matsuka,Zhijun Zhang,Eiji Tanaka,Akihito Yamamoto

Journal of neuropathology and experimental neurology 80:530-540 PubMed33990839

2021

Cytoplasmic Mislocalization of RNA Polymerase II Subunit RPB1 in Alzheimer Disease Is Linked to Pathologic Tau.

Applications

Unspecified application

Species

Unspecified reactive species

John R Dickson,Hyejin Yoon,Matthew P Frosch,Bradley T Hyman
View all publications
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