Goat Anti-Rat IgG H&L (Alexa Fluor® 594)

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (AB150160)

ab252885 staining Strep-tag II in Strep tag-GFP transfected HEK-293T cells. The cells were fixed with 4% paraformaldehyde and permeabilised in 0.1% Triton X-100. The cells were then incubated with ab252885 at 1/250 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Nucleus were visualized using DAPI. Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (AB150160)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Poly PR with ab323367 at 1/500 (1.91 ug/ml) dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing nuclear with cytoplasmic staining in 293T cells transfected with a poly PR expression vector containing a EGFP-tag®(shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody only control : Secondary antibody is ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) at 1/1000 2ug/ml dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (AB150160)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse splenocyte cells labelling LY6C with ab305229 at a 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor^® 488) (ab150081) secondary antibody at a 1/1000 dilution. Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green), which is partially co-located with CD11b. Nuclear DNA was labeled with DAPI (shown in blue).

Counterstained with Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/1000 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor^® 594) (ab150160) at a 1/1000 dilution (shown in magenta).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The negative controls are as follows :

-ve control 1 : LY6C (ab305229) at a 1/100 dilution, followed by Goat anti-Rat IgG H&L (Alexa Fluor^® 594) (ab150160) secondary at a 1/1000 dilution.

-ve control 2 : Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor^® 488) (ab150081) at a 1/1000 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (AB150160)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150160 Alexa Fluor® 594 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (AB150160)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150160 Alexa Fluor® 594 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• Alexa Fluor®

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Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (AB150160)

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (AB150160)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse bone marrow cells labelling Lactoferrin with ab325119 at 1/50000 (0.01 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in subset of CD11b+mouse bone marrows (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab325119 at 1/50000 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2 : ab8878 at 1/200 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.