Goat Anti-Rat IgG H&L (Alexa Fluor® 594) is a secondary antibody with a maximum absorption wavelength of 590nm and a maximum emission wavelength of 617nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 90 publications
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- Respiratory research 25:62024CD147 induces asthmatic airway remodeling and activation of circulating fibrocytes in a mouse model of asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao Li,Tao Cheng,Yaning Guo,Rong Gao,Xuankun Ma,Xuecong Mao,Xinpeng HanPubMed 38178133
- Hippocampus 34:156-1652023Hippocampal parvalbumin and perineuronal nets: Possible involvement in anxiety-like behavior in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhixin Fan,Xiayu Gong,Hanfang Xu,Yue Qu,Bozhi Li,Lanxin Li,Yuqi Yan,Lili Wu,Can YanPubMed 38100162
- International journal of nanomedicine 18:7267-72852023Drug Repositioning of Metformin Encapsulated in PLGA Combined with Photothermal Therapy Ameliorates Rheumatoid Arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDae Kyu Kim,Jun Young Park,Youn Joo Kang,Dongwoo KhangPubMed 38090362
- Psychopharmacology 241:687-6982023Liraglutide ameliorates delirium-like behaviors of aged mice undergoing cardiac surgery by mitigating microglia activation via promoting mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jia,Xin Lv,Tong Zhu,Jin-Chun Shen,Wen-Xue Liu,Jian-Jun YangPubMed 37968531
- Biomedical microdevices 25:402023Role of interleukin 6 and its soluble receptor on the diffusion barrier dysfunction of alveolar tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Sui,Woo LeePubMed 37851124
- Bioactive materials 32:12-272023immunomodulation of tumors with biosynthetic bacteria promote anti-tumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongda Lin,Fanqiang Meng,Yumeng Ma,Chi Zhang,Zhirang Zhang,Zhaoxin Yang,Yuan Li,Linlin Hou,Yuzhong Xu,Xin Liang,Xudong ZhangPubMed 37790917
- Cell death discovery 9:2692023IL4I1-catalyzed tryptophan metabolites mediate the anti-inflammatory function of cytokine-primed human muscle stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuqiu Zuo,Jiankai Fang,Peiqing Huang,Shisong Liu,Pengbo Hou,Shiqing Wang,Zhanhong Liu,Chao Feng,Lijuan Cao,Peishan Li,Yufang Shi,Changshun ShaoPubMed 37507432
- Science advances 9:eabq07122023CD4 T cells aggravate hemorrhagic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel X Shi,Yuwen Xiu,Yan Li,Meng Yuan,Kaibin Shi,Qiang Liu,Xiaoying Wang,Wei-Na JinPubMed 37285421
- Clinical and translational medicine 13:e12352023Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnpei Hu,Guo Chen,Banghe Bao,Yanhua Guo,Dan Li,Xiaojing Wang,Jianqun Wang,Qilan Li,Yi Zhou,Haiyang Gao,Jiyu Song,Xinyi Du,Liduan Zheng,Qiangsong TongPubMed 37186134
- Neural regeneration research 18:2315-23202023Adipose mesenchymal stem cell-derived extracellular vesicles reduce glutamate-induced excitotoxicity in the retina.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian-Qi Duan,Zhao-Lin Gao,Ai-Xiang Luo,Dan Chen,Jian-Bin Tong,Ju-Fang HuangPubMed 37056153
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, IHC-FoFr, ICC/IF, Flow Cyt, IHC-P, ELISA
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-FoFr | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Alternative names
Ig gamma-1 chain C region
Recommended products
Goat Anti-Rat IgG H&L (Alexa Fluor® 594) is a secondary antibody with a maximum absorption wavelength of 590nm and a maximum emission wavelength of 617nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 90 publications
Key facts
- Description
- Goat Anti-Rat IgG H&L (Alexa Fluor® 594)
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, IHC-FoFr, ICC/IF, Flow Cyt, IHC-P, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Rat IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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6 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150160 Alexa Fluor® 594 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150160 Alexa Fluor® 594 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Poly PR with Anti-Rat Poly PR antibody [PERA534G] ab323367 at 1/500 (1.91 ug/ml) dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing nuclear with cytoplasmic staining in 293T cells transfected with a poly PR expression vector containing a EGFP-tag®(shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) at 1/1000 2ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse splenocyte cells labelling LY6C with Anti-LY6C antibody [EPR27220-67] ab305229 at a 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at a 1/1000 dilution. Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green), which is partially co-located with CD11b. Nuclear DNA was labeled with DAPI (shown in blue).
Counterstained with Anti-CD11b Rat monoclonal antibody (Anti-CD11b antibody [M1/70] ab8878) at a 1/1000 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160) at a 1/1000 dilution (shown in magenta).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The negative controls are as follows:
-ve control 1: LY6C (Anti-LY6C antibody [EPR27220-67] ab305229) at a 1/100 dilution, followed by Goat anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160) secondary at a 1/1000 dilution.
-ve control 2: Anti-CD11b Rat monoclonal antibody (Anti-CD11b antibody [M1/70] ab8878) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at a 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)
Anti-Strep-tag II antibody [11A7] ab252885 staining Strep-tag II in Strep tag-GFP transfected HEK-293T cells. The cells were fixed with 4% paraformaldehyde and permeabilised in 0.1% Triton X-100. The cells were then incubated with Anti-Strep-tag II antibody [11A7] ab252885 at 1/250 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Nucleus were visualized using DAPI.
Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector.
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