Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.
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- Nature communications 14:65692023Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Wei Chou,Chia-Nung Hung,Cheryl Hsiang-Ling Chiu,Xi Tan,Meizhen Chen,Chien-Chin Chen,Moawiz Saeed,Che-Wei Hsu,Michael A Liss,Chiou-Miin Wang,Zhao Lai,Nathaniel Alvarez,Pawel A Osmulski,Maria E Gaczynska,Li-Ling Lin,Veronica Ortega,Nameer B Kirma,Kexin Xu,Zhijie Liu,Addanki P Kumar,Josephine A Taverna,Gopalrao V N Velagaleti,Chun-Liang Chen,Zhao Zhang,Tim Hui-Ming HuangPubMed 37848444
- Ecotoxicology and environmental safety 259:1150422023HMGB 1 acetylation mediates trichloroethylene-induced immune kidney injury by facilitating endothelial cell-podocyte communication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuesong Zhang,Haibo Xie,Zhibing Liu,Jiaxiang Zhang,Lihua Deng,Qifeng Wu,Yuansheng Duan,Feng Wang,Changhao Wu,Qixing ZhuPubMed 37216866
- Scientific reports 11:183352021Mesenchymal stromal cells as carriers of IL-12 reduce primary and metastatic tumors of murine melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Kułach,Ewelina Pilny,Tomasz Cichoń,Justyna Czapla,Magdalena Jarosz-Biej,Marek Rusin,Alina Drzyzga,Sybilla Matuszczak,Stanisław Szala,Ryszard SmolarczykPubMed 34526531
- Cancers 13:2021The Proper Administration Sequence of Radiotherapy and Anti-Vascular Agent-DMXAA Is Essential to Inhibit the Growth of Melanoma Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlina Drzyzga,Tomasz Cichoń,Justyna Czapla,Magdalena Jarosz-Biej,Ewelina Pilny,Sybilla Matuszczak,Piotr Wojcieszek,Zbigniew Urbaś,Ryszard SmolarczykPubMed 34439079
- The Journal of biological chemistry 295:17497-175132021Fibrillar α-synuclein toxicity depends on functional lysosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie J Guiney,Paul A Adlard,Peng Lei,Celeste H Mawal,Ashley I Bush,David I Finkelstein,Scott AytonPubMed 33453994
- eLife 9:2020The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Huang et. al.PubMed 32374261
- BMC biology 17:672019Analysis of the genetically tractable crustacean Parhyale hawaiensis reveals the organisation of a sensory system for low-resolution vision.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Patricia Ramos et. al.PubMed 31416484
- Nucleic acids research 47:5670-56832019SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongbin Liu,Tao Huang,Mengjing Li,Miao Li,Chuanxin Zhang,Jing Jiang,Xiaochen Yu,Yingying Yin,Fan Zhang,Gang Lu,Meng-Cheng Luo,Liang-Ran Zhang,Jinsong Li,Kui Liu,Zi-Jiang ChenPubMed 30949703
- Scientific reports 7:10862017In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Si,Debasish Sen,Rebecca Dutta,Siavash Yousefi,Roopa Dalal,Yonatan Winetraub,Orly Liba,Adam de la ZerdaPubMed 28439123
- Journal of orthopaedic research : official publication of the Orthopaedic Research Society 35:524-5362016Early changes in the knee of healer and non-healer mice following non-invasive mechanical injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Duan,Muhammad Farooq Rai,Nilsson Holguin,Matthew J Silva,Debabrata Patra,Weiming Liao,Linda J SandellPubMed 27591401
Key facts
- Host species
Goat
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Chicken, Cow, Human
- Conjugation
Alexa Fluor® 594
- Excitation/Emission
Ex: 590nm, Em: 617nm
- Applications
ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.
Key facts
- Description
Goat Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed
- Host species
Goat
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Chicken, Cow, Human
- Conjugation
Alexa Fluor® 594
- Excitation/Emission
Ex: 590nm, Em: 617nm
- Applications
ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
Polyclonal
- Pre-adsorbed
Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chain common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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3 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (ab150168)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150168 Alexa Fluor® 594 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (ab150168)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150168 Alexa Fluor® 594 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (ab150168)
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