Goat Anti-Rat IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 40 publications
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- Cellular & molecular biology letters 29:242024The direct binding of bioactive peptide Andersonin-W1 to TLR4 expedites the healing of diabetic skin wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Li,Yuxin Xiong,Zhe Fu,Yuxin Ji,Jiayi Yan,Yan Kong,Ying Peng,Zeqiong Ru,Yubing Huang,Yilin Li,Ying Yang,Li He,Jing Tang,Ying Wang,Xinwang YangPubMed 38317065
- International journal of molecular medicine 53:2024NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Zhou,Qun Zhang,Chenyang Liu,Jiahao Fan,Wen Huang,Nan Li,Mingxia Yang,Hong Wang,Weiping Xie,Hui KongPubMed 38240085
- Cellular & molecular biology letters 28:612023A frog peptide provides new strategies for the intervention against skin wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Li,Zhe Fu,Tao Jin,Yixiang Liu,Naixin Liu,Saige Yin,Zhuo Wang,Yubing Huang,Yinglei Wang,Yingxuan Zhang,Jiayi Li,Yutong Wu,Li He,Jing Tang,Ying Wang,Xinwang YangPubMed 37501100
- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2305-23252023A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing He,Heng Shi,Ge Zhang,Yujie Peng,Avirup Ghosh,Mengfan Zhang,Xiaofeng Hu,Chunhua Liu,Yue Shao,Shujie Wang,Lijiang Chen,Wenjian Sun,Junfeng Su,Xi Chen,Liang Zhang,Ying-Shing Chan,Duanqing Pei,Micky Tortorella,Yiping Guo,Hong Yan,Jufang HePubMed 36813575
- STAR protocols 4:1017572022Protocol for wireless deep brain stimulation in freely behaving mice with infrared light.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Wu,Guosong HongPubMed 36538396
- eLife 11:2022The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristiane Pleuger,Dingding Ai,Minea L Hoppe,Laura T Winter,Daniel Bohnert,Dominik Karl,Stefan Guenther,Slava Epelman,Crystal Kantores,Monika Fijak,Sarina Ravens,Ralf Middendorff,Johannes U Mayer,Kate L Loveland,Mark Hedger,Sudhanshu Bhushan,Andreas MeinhardtPubMed 36515584
- Journal of neuroinflammation 19:2532022Promoted CD4 T cell-derived IFN-γ/IL-10 by photobiomodulation therapy modulates neurogenesis to ameliorate cognitive deficits in APP/PS1 and 3xTg-AD mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolei Wu,Qi Shen,Haocai Chang,Junyu Li,Da XingPubMed 36217178
- The Journal of biological chemistry 298:1022922022SUMOylation of microtubule-cleaving enzyme KATNA1 promotes microtubule severing and neurite outgrowth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaojin Li,Yaozhong Liang,Jianyu Zou,Zhenbin Cai,Hua Yang,Jie Yang,Yunlong Zhang,Hongsheng Lin,Guowei Zhang,Minghui TanPubMed 35868557
- Nature communications 13:39812022Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Antel,Romir Raj,Madona Y G Masoud,Ziwei Pan,Sheng Li,Barbara G Mellone,Mayu InabaPubMed 35810185
- Scientific reports 12:115802022A medium-chain triglyceride containing ketogenic diet exacerbates cardiomyopathy in a CRISPR/Cas9 gene-edited rat model with Duchenne muscular dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuri Fujikura,Koichi Kimura,Keitaro Yamanouchi,Hidetoshi Sugihara,Masaki Hatakeyama,Haotong Zhuang,Tomoki Abe,Masao Daimon,Hiroyuki Morita,Issei Komuro,Katsutaka OishiPubMed 35803994
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Goat Anti-Rat IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 40 publications
Key facts
- Description
- Goat Anti-Rat IgG H&L (Alexa Fluor® 647)
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Rat IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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4 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) (ab150159)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (red) was ab150159 Alexa Fluor® 647 goat anti-rat IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) (ab150159)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) (ab150159)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Myelin PLP with Alexa Fluor® 488 Anti-Myelin PLP antibody [EPR23504-106] ab319182 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in rat primary glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection.
Anti-Myelin Basic Protein rat monoclonal antibody was used to counterstain tubulin (Magenta). The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) (ab150159)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Myelin PLP with Alexa Fluor® 488 Anti-Myelin PLP antibody [EPR23504-106] ab319182 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in mouse primary glia cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection.
Anti-Myelin Basic Protein rat monoclonal antibody was used to counterstain tubulin (Magenta). The Nuclear counterstain was DAPI (Blue).
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