Goat Anti-Rat IgG H&L (Alexa Fluor® 647) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
Images
Publications
Filter by
- Frontiers in molecular neuroscience 16:12141502023Huntingtin-associated protein 1 ameliorates neurological function rehabilitation by facilitating neurite elongation through TrKA-MAPK pathway in mice spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Miao,Sun Wan Qing,Lu TaoPubMed 37609072
- PloS one 18:e02888002023Pro-fibrogenic and adipogenic aspects of chronic muscle degeneration are contributed by distinct stromal cell subpopulations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCansu Özdemir,Duygu Akçay,Diğdem Yöyen-Ermiş,Ekim Zihni Taşkıran,Rana Soylu-Kucharz,Güneş Esendağlı,Yusuf Çetin KocaefePubMed 37463149
- JCI insight 8:2023Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiya Yang,Robert H Brown,Dan Wang,Kevin A Strauss,Guangping GaoPubMed 37014712
- iScience 26:1067602023Restoration of normal blood flow in atherosclerotic arteries promotes plaque stabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorgan A Schake,Ian S McCue,Evan T Curtis,Thomas J Ripperda,Samuel Harvey,Bryan T Hackfort,Anna Fitzwater,Yiannis S Chatzizisis,Forrest M Kievit,Ryan M PedrigiPubMed 37235059
- Disease models & mechanisms 16:2023Upregulation of Tribbles decreases body weight and increases sleep duration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebeka Popovic,Yizhou Yu,Nuno Santos Leal,Giorgio Fedele,Samantha H Y Loh,L Miguel MartinsPubMed 37083954
- iScience 26:1063402023Global sphingosine-1-phosphate receptor 2 deficiency attenuates neuroinflammation and ischemic-reperfusion injury after neonatal stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumi Fukuzaki,Joel Faustino,Matthieu Lecuyer,Aditya Rayasam,Zinaida S VexlerPubMed 37009213
- Cell & bioscience 13:232023Ang-(1-7)/MasR axis promotes functional recovery after spinal cord injury by regulating microglia/macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangjin Gu,Bin Zhu,Jie Ren,Xiaomeng Song,Baoyou Fan,Han Ding,Jun Shang,Heng Wu,Junjin Li,Hongda Wang,Jinze Li,Zhijian Wei,Shiqing FengPubMed 36739421
- Bioactive materials 21:595-6112023Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Wu,Xinghui Wei,Yichao Liu,Hui Dong,Zhen Tang,Ning Wang,Shusen Bao,Zhigang Wu,Lei Shi,Xiongfei Zheng,Xiaokang Li,Zheng GuoPubMed 36685731
- Nature communications 13:72422022Spatially resolved proteomics via tissue expansion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Li,Cuiji Sun,Yaoting Sun,Zhen Dong,Runxin Wu,Xiaoting Sun,Hanbin Zhang,Wenhao Jiang,Yan Zhou,Xufeng Cen,Shang Cai,Hongguang Xia,Yi Zhu,Tiannan Guo,Kiryl D PiatkevichPubMed 36450705
- Cancers 14:2022Endothelial-Specific Molecule 1 Inhibition Lessens Productive Angiogenesis and Tumor Metastasis to Overcome Bevacizumab Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Kang,Xue Liang,Buxi Fan,Chen Zhao,Beiyu Shen,Xuemei Ji,Yu LiuPubMed 36428773
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Sheep, Rabbit, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Goat Anti-Rat IgG H&L (Alexa Fluor® 647) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
Key facts
- Description
- Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Sheep, Rabbit, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chain common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Rat IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150167 Alexa Fluor® 647 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab150167 Alexa Fluor® 647 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
Immunohistochemistry (Frozen sections) - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
Immunohistochemical analysis of 10% PFA-fixed, 0.1% Triton X-100 permeabilized frozen Human colon (fresh) tissue labeling CEACAM1 + CEACAM3 + CEACAM6 with Anti-CEACAM1 + CEACAM3 + CEACAM6 antibody [YTH71.3] ab104450 at 1/200 dilution followed by ab150167 Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution.
Confocal image showing positive staining on human colon. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150167 Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com