Suitable for Dot, IHC-Fr, IHC-P, IM, ICC/IF, WB, ELISA. Cited in 4 publications.
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Publications
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- Journal of lipid research 64:1004502023Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Ichi Morishige,Kazuaki Yoshioka,Hiroki Nakata,Kazuhiro Ishimaru,Naoto Nagata,Tamotsu Tanaka,Yoh Takuwa,Hitoshi AndoPubMed 37751791
- PloS one 17:e02645722022Topical estrogen application promotes cutaneous wound healing in db/db female mice with type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanae Mukai,Shin-Ichi Horike,Makiko Meguro-Horike,Yukari Nakajima,Arya Iswara,Toshio NakataniPubMed 35271602
- PloS one 13:e01985312018Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLoreen R Stromberg et. al.PubMed 29902192
- Insects 3:1190-92012Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew R Tarver et. al.PubMed 26466734
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- Dot, IHC-Fr, IHC-P, IM, ICC/IF, WB, ELISA
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/200 - 1/1000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/500 - 1/2500 | Notes - |
Application ELISA | Reactivity Expected | Dilution info 1/15000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for Dot, IHC-Fr, IHC-P, IM, ICC/IF, WB, ELISA. Cited in 4 publications.
Key facts
- Description
- Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- Dot, IHC-Fr, IHC-P, IM, ICC/IF, WB, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Part of the AbExcel range.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846)
All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
SecondaryLanes 1 - 2: Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/5000 dilution
Lanes 3 - 4: Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/20000 dilution
Lanes 5 - 6: Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846)
Western blot analysis of ab6846 used as a secondary antibody at 1/1000 dilution for 60 min at RT.
All lanes: Rat IgG at 50 ng
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/1000 dilution
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Product protocols
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