AB6844

Goat Anti-Rat IgG H&L (Biotin)

Name: Goat Anti Rat (IgG) secondary antibody Biotin (ab6844) | Abcam
Brand: Abcam Limited
SKU: AB6844
Price: 265 USD
Availability: InStock

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(11 Publications)

Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 11 publications.

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (AB6844)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (AB6844)

ab6844 was used at dilution 1/500 with the primary antibody ab6640 in IHC-P. See the review on ab6640.

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (Biotin) (AB6844)

Western blot analysis of ab6844. Secondary antibody : HRP Conjugated Streptavidin at 1/40000 dilution for 30 min at RT.

All lanes:

Western blot - Goat Anti-Rat IgG H&L (Biotin) (ab6844) at 1/1000 dilution

All lanes:

Rat IgG at 100 ng

Secondary

All lanes:

HRP Conjugated Streptavidin at 1/40000 dilution

false

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

ICC/IF, IHC-Fr, IHC-P, IM, ELISA, Dot, WB

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 26:717-728 PubMed39286641

2024

Laminin 511 E8 fragment promotes to form basement membrane-like structure in human skin equivalents.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Fujisaki,Takafumi Watanabe,Shusuke Yoshihara,Hideki Fukuda,Yasuko Tomono,Chisa Tometsuka,Kazunori Mizuno,Toshio Nishiyama,Shunji Hattori

Nature communications 13:779 PubMed35140209

2022

Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis by dysregulating redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Donghyun Kang,Jeeyeon Lee,Jisu Jung,Bradley A Carlson,Moon Jong Chang,Chong Bum Chang,Seung-Baik Kang,Byung Cheon Lee,Vadim N Gladyshev,Dolph L Hatfield,Byeong Jae Lee,Jin-Hong Kim

Clinical and translational medicine 11:e305 PubMed33634991

2021

Restoration of renal TIMP3 levels via genetics and pharmacological approach prevents experimental diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana Casagrande,Giulia Iuliani,Stefano Menini,Giuseppe Pugliese,Massimo Federici,Rossella Menghini

Molecular oncology : PubMed33021054

2020

Optimized low-dose combinatorial drug treatment boosts selectivity and efficacy of colorectal carcinoma treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Marloes Zoetemelk,George M Ramzy,Magdalena Rausch,Thibaud Koessler,Judy R van Beijnum,Andrea Weiss,Valentin Mieville,Sander R Piersma,Richard R de Haas,Céline Delucinge-Vivier,Axel Andres,Christian Toso,Alexander A Henneman,Simone Ragusa,Tatiana V Petrova,Mylène Docquier,Thomas A McKee,Connie R Jimenez,Youssef Daali,Arjan W Griffioen,Laura Rubbia-Brandt,Pierre-Yves Dietrich,Patrycja Nowak-Sliwinska

Oncology reports 42:679-687 PubMed31233194

2019

MPZL1 promotes tumor cell proliferation and migration via activation of Src kinase in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Danni Chen,Lei Cao,Xiaojie Wang

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 31:1218-25 PubMed23568545

2013

Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus.

Applications

Unspecified application

Species

Unspecified reactive species

Johannah Sanchez-Adams,Rebecca E Wilusz,Farshid Guilak

Journal of the American Society of Nephrology : JA 23:1835-46 PubMed23085633

2012

Activation of liver X receptor inhibits osteopontin and ameliorates diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hiromi Tachibana,Daisuke Ogawa,Yuichi Matsushita,Dennis Bruemmer,Jun Wada,Sanae Teshigawara,Jun Eguchi,Chikage Sato-Horiguchi,Haruhito Adam Uchida,Kenichi Shikata,Hirofumi Makino

The international journal of neuropsychopharmacology 16:1033-44 PubMed22932447

2012

The evaluation of AZ66, an optimized sigma receptor antagonist, against methamphetamine-induced dopaminergic neurotoxicity and memory impairment in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Seminerio,Rolf Hansen,Nidhi Kaushal,Han-Ting Zhang,Christopher R McCurdy,Rae R Matsumoto

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 23:960-71 PubMed22921523

2012

Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Nidhi Kaushal,Michael J Seminerio,Matthew J Robson,Christopher R McCurdy,Rae R Matsumoto

Journal of immunology (Baltimore, Md. : 1950) 185:688-97 PubMed20519655

2010

Purinergic receptor inhibition prevents the development of smoke-induced lung injury and emphysema.

Applications

IHC-P

Species

Unspecified reactive species

Sanja Cicko,Monica Lucattelli,Tobias Müller,Marek Lommatzsch,Giovanna De Cunto,Silvia Cardini,William Sundas,Melanine Grimm,Robert Zeiser,Thorsten Dürk,Gernot Zissel,Jean-Marie Boeynaems,Stephan Sorichter,Davide Ferrari,Francesco Di Virgilio,J Christian Virchow,Giuseppe Lungarella,Marco Idzko

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Rat IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (AB6844)

Western blot - Goat Anti-Rat IgG H&L (Biotin) (AB6844)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise