AB6953

Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed

Name: Goat Anti Rat (IgG) secondary antibody preadsorbed Cy3® (ab6953) | Abcam
Brand: Abcam Limited
SKU: AB6953
Price: 295 USD
Availability: InStock

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(22 Publications)

Suitable for Flow Cyt, ICC, IHC-Fr, IHC-P, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 22 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Applications

ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Fr, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region.

P20759

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Product details

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:357-367 PubMed39866229

2025

Melanoma cell line-derived exosomal miR-424-5p: a key promoter of angiogenesis through LATS2 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei DU,Qiang Zhang,Jing Zhang,Maierdanjiang Maihemuti,Haiyang He,Renbing Jiang

Frontiers in immunology 14:1273556 PubMed38193079

2024

Maternal consumption of a high-fat diet modulates the inflammatory response in their offspring, mediated by the M1 muscarinic receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Suleyma Oliveira Costa,Wenicios Ferreira Chaves,Priscilla Karla Fernandes Lopes,Iracema M Silva,Beatriz Burguer,Leticia M Ignácio-Souza,Adriana Souza Torsoni,Marciane Milanski,Hosana Gomes Rodrigues,Mina Desai,Michael Glenn Ross,Marcio Alberto Torsoni

Cell genomics 3:100409 PubMed37868034

2023

Molecular signature incorporating the immune microenvironment enhances thyroid cancer outcome prediction.

Applications

Unspecified application

Species

Unspecified reactive species

George J Xu,Matthew A Loberg,Jean-Nicolas Gallant,Quanhu Sheng,Sheau-Chiann Chen,Brian D Lehmann,Sophia M Shaddy,Megan L Tigue,Courtney J Phifer,Li Wang,Mario W Saab-Chalhoub,Lauren M Dehan,Qiang Wei,Rui Chen,Bingshan Li,Christine Y Kim,Donna C Ferguson,James L Netterville,Sarah L Rohde,Carmen C Solórzano,Lindsay A Bischoff,Naira Baregamian,Aaron C Shaver,Mitra Mehrad,Kim A Ely,Daniel W Byrne,Thomas P Stricker,Barbara A Murphy,Jennifer H Choe,Luciane T Kagohara,Elizabeth M Jaffee,Eric C Huang,Fei Ye,Ethan Lee,Vivian L Weiss

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 27:437-448 PubMed37641806

2023

Paeonol accelerates skin wound healing by regulating macrophage polarization and inflammation in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zuyang Zhang,Tianhua Chen,Wei Liu,Jiepeng Xiong,Liangdong Jiang,Mingjiang Liu

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 20:1154-1166 PubMed37133631

2023

Inhibition of ACSL4 Alleviates Parkinsonism Phenotypes by Reduction of Lipid Reactive Oxygen Species.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Tang,Liu-Yao Zhou,Ping Li,Ling-Ling Jiao,Kang Chen,Yu-Jie Guo,Xu-Long Ding,Si-Yu He,Biao Dong,Ru-Xiang Xu,Huan Xiong,Peng Lei

Psychopharmacology 240:1179-1190 PubMed36949340

2023

Curcumin protects against doxorubicin induced oxidative stress by regulating the Keap1-Nrf2-ARE and autophagy signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Dehua Liao,Danggang Shangguan,Yi Wu,Yun Chen,Ni Liu,Jingyi Tang,Dunwu Yao,Yingrui Shi

CNS neuroscience & therapeutics 29:2145-2161 PubMed36914965

2023

Protein arginine methyltransferase 8 regulates ferroptosis and macrophage polarization in spinal cord injury via glial cell-derived neurotrophic factor.

Applications

Unspecified application

Species

Unspecified reactive species

Zehua Zou,Ruixuan Liu,Yiwen Wang,Hongjian Tan,Gang An,Baifeng Zhang,Yongzhi Wang,Daming Dong

Laboratory investigation; a journal of technical methods and pathology 102:691-701 PubMed35013529

2022

Induction of cancer cell stemness in glioma through glycolysis and the long noncoding RNA HULC-activated FOXM1/AGR2/HIF-1α axis.

Applications

Unspecified application

Species

Unspecified reactive species

You-Ping Li,Yue Liu,Li-Min Xiao,Li-Ke Chen,Er-Xing Tao,Er-Ming Zeng,Chun-Hua Xu

Annals of translational medicine 9:974 PubMed34277774

2021

Hydrogen-rich saline regulates the polarization and apoptosis of alveolar macrophages and attenuates lung injury via suppression of autophagy in septic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Qiu,Yang Liu,Keyan Chen,Youjing Dong,Shiqing Liu,Jin Zhang

Frontiers in cell and developmental biology 8:553728 PubMed33195189

2020

Geniposide Alleviates Oxidative Stress of Mice With Depression-Like Behaviors by Upregulating Six3os1.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Zou,Kazuo Sugimoto,Jielin Zhang,Yongxiu Liu,Yiming Zhang,Hao Liang,Yinan Jiang,Jing Wang,Guoxiang Duan,Cheng Mei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (22)

Product promise