AB6565

Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed

Name: Goat Anti-Rat IgG (Cy5 ®) preadsorbed secondary antibody (ab6565) | Abcam
Brand: Abcam Limited
SKU: AB6565
Price: 295 USD
Availability: InStock

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(49 Publications)

Suitable for Flow Cyt, ICC, IHC-Fr, IHC-P, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 49 publications.

View Alternative Names

Ig gamma-1 chain C region

1 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (AB6565)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (AB6565)

ab6565 was used at dilution 1/500 with the primary antibody ab6161 in ICC/IF. See the review on ab6161.

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Cy5®

Excitation/Emission

Ex: 650nm, Em: 667nm

Applications

Flow Cyt, IHC-P, ICC/IF, IHC-Fr, ICC, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Product details

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (49)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40608419

2025

Cyclin D1 overexpression induces replication stress and microhomology-mediated end-joining dependence in mantle cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jithma P Abeykoon,Shuhei Asada,Guangli Zhu,Yuna Hirohashi,Lisa Moreau,Divya Iyer,Sirisha Mukkavalli,Kalindi Parmar,Gabriella Zambrano,Lige Jiang,Dongni Yi,Michelle Manske,Kimberly Gwin,Rebecca L King,James R Cerhan,Xiaosheng Wu,Zhenkun Lou,Geoffrey I Shapiro,Thomas Witzig,Alan D'Andrea

Iranian journal of pharmaceutical research : IJPR 23:e148139 PubMed40066121

2025

The Glucagon-Like Peptide-1 (GLP-1) Receptor Agonist Liraglutide Regulates Sirtuin-1-Mediated Neutrophil Extracellular Traps to Improve Diabetes-Induced Bone Metabolism Imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zhong,Liangzhi Huang,Tingting Lin,Yanyan Li,Bin Deng,Dezhi Kong,Zhanlin Liao,Zugui Huang

Cell reports 44:115360 PubMed40014449

2025

Translesion-synthesis-mediated bypass of DNA lesions occurs predominantly behind replication forks restarted by PrimPol.

Applications

Unspecified application

Species

Unspecified reactive species

Ashna Dhoonmoon,Julia R Ambrose,Sonal Garg,Cynthia Lascarez-Espana,Abbey Rebok,Thomas E Spratt,George-Lucian Moldovan,Claudia M Nicolae

PLoS biology 23:e3002955 PubMed39854351

2025

Rspo3-mediated metabolic liver zonation regulates systemic glucose metabolism and body mass in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Uno,Takuya Uchino,Takashi Suzuki,Yohei Sayama,Naoki Edo,Kiyoko Uno-Eder,Koji Morita,Toshio Ishikawa,Miho Koizumi,Hiroaki Honda,Hideki Katagiri,Kazuhisa Tsukamoto

Science advances 11:eads2919 PubMed39792662

2025

Flap endonuclease 1 repairs DNA-protein cross-links via ADP-ribosylation-dependent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yilun Sun,Lisa M Jenkins,Lara H El Touny,Linying Zhu,Xi Yang,Ukhyun Jo,Lauren Escobedo,Tapan K Maity,Liton Kumar Saha,Isabel Uribe,Sourav Saha,Shunichi Takeda,Anthony K L Leung,Ken Cheng,Yves Pommier

Nucleic acids research 52:13003-13018 PubMed39413208

2024

SNF2L suppresses nascent DNA gap formation to promote DNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Nelligan,Huzefa Dungrawala

International journal of molecular sciences 25: PubMed39337629

2024

Phenotypic, Metabolic, and Functional Characterization of Experimental Models of Foamy Macrophages: Toward Therapeutic Research in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Amina Sarah Henni Mansour,Mathilde Ragues,Julien Brevier,Coraline Borowczyk,Janaïna Grevelinger,Jeanny Laroche-Traineau,Johan Garaude,Sébastien Marais,Marie-Josée Jacobin-Valat,Edouard Gerbaud,Gisèle Clofent-Sanchez,Florence Ottones

Nature communications 15:7404 PubMed39191776

2024

Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Anchel de Jaime-Soguero,Janina Hattemer,Anja Bufe,Alexander Haas,Jeroen van den Berg,Vincent van Batenburg,Biswajit Das,Barbara di Marco,Stefania Androulaki,Nicolas Böhly,Jonathan J M Landry,Brigitte Schoell,Viviane S Rosa,Laura Villacorta,Yagmur Baskan,Marleen Trapp,Vladimir Benes,Andrei Chabes,Marta Shahbazi,Anna Jauch,Ulrike Engel,Annarita Patrizi,Rocio Sotillo,Alexander van Oudenaarden,Josephine Bageritz,Julieta Alfonso,Holger Bastians,Sergio P Acebrón

Nucleic acids research 52:10999-11013 PubMed39180395

2024

Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair.

Applications

Unspecified application

Species

Unspecified reactive species

Erica Colicino-Murbach,Caitlin Hathaway,Huzefa Dungrawala

Cancer research 84:3435-3446 PubMed38885312

2024

Single-Stranded DNA Gap Accumulation Is a Functional Biomarker for USP1 Inhibitor Sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre A da Costa,Ozge Somuncu,Ramya Ravindranathan,Sirisha Mukkavalli,David B Martignetti,Huy Nguyen,Yuqing Jiao,Benjamin P Lamarre,Golbahar Sadatrezaei,Lisa Moreau,Joyce Liu,Divya R Iyer,Jean-Bernard Lazaro,Geoffrey I Shapiro,Kalindi Parmar,Alan D D'Andrea

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (AB6565)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (49)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise