Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Cited in 37 publications.
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- Cancer cell international 23:2922023Holothurian triterpene glycoside cucumarioside A-2 induces macrophages activation and polarization in cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Han Chuang,Evgeny Pislyagin,Liang-Yu Lin,Ekaterina Menchinskaya,Oleg Chernikov,Valery Kozhemyako,Tatiana Gorpenchenko,Igor Manzhulo,Elena Chaikina,Irina Agafonova,Alexandra Silchenko,Sergey Avilov,Valentin Stonik,Shey-Cherng Tzou,Dmitry Aminin,Yun-Ming WangPubMed 38001420
- Renal failure 45:22539242023Crocin improves the renal autophagy in rat experimental membranous nephropathy via regulating the SIRT1/Nrf2/HO-1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Liu,Hong Cheng,Hongyun Wang,Qiong Wang,Jun YuanPubMed 37724538
- PeerJ 11:e158942023Tibial cortex transverse transport potentiates diabetic wound healing activation of SDF-1/CXCR4 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuanji Ou,Xiaodong Wu,Yang Yang,Changliang Xia,Wei Zhang,Yudun Qu,Jiaxuan Li,Bo Chen,Lilin Zhu,Changpeng Xu,Yong QiPubMed 37727693
- Cellular & molecular biology letters 28:152023IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiangwei Xiao,Jingwen Huang,Xiaoting Jian,Han Wang,Haiqiang Lan,Zhaohong Liao,Ruicai Gu,Jijie Hu,Hua LiaoPubMed 36849929
- Heliyon 9:e137772023Neuroprotection by Nrf2 via modulating microglial phenotype and phagocytosis after intracerebral hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuntian Liang,Lirong Liu,Shuangjin Bao,Zhenjia Yao,Qinqin Bai,Pengcheng Fu,Xiangyu Liu,John H Zhang,Gaiqing WangPubMed 36852060
- Cell and tissue research 391:189-2032022Hypertrophic and fibrotic human PKD hearts are associated with macrophage infiltration and abnormal TGF-β signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFarideh Amirrad,Gregory A Fishbein,Robert A Edwards,Surya M NauliPubMed 36376769
- Acta neuropathologica communications 10:412022AQP4 labels a subpopulation of white matter-dependent glial radial cells affected by pediatric hydrocephalus, and its expression increased in glial microvesicles released to the cerebrospinal fluid in obstructive hydrocephalus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeandro Castañeyra-Ruiz,Ibrahim González-Marrero,Luis G Hernández-Abad,Emilia M Carmona-Calero,Marta R Pardo,Rebeca Baz-Davila,Seunghyun Lee,Michael Muhonen,Ricardo Borges,Agustín Castañeyra-PerdomoPubMed 35346374
- Nature communications 12:71062021Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex M Yoshikawa,Alexandra Rangel,Trevor Feagin,Elizabeth M Chun,Leighton Wan,Anping Li,Leonhard Moeckl,Diana Wu,Michael Eisenstein,Sharon Pitteri,H Tom SohPubMed 34876561
- Frontiers in cardiovascular medicine 8:7729612021Arrhythmogenic Hearts in PKD2 Mutant Mice Are Characterized by Cardiac Fibrosis, Systolic, and Diastolic Dysfunctions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFarideh Amirrad,Rajasekharreddy Pala,Kiumars Shamloo,Brian S Muntean,Surya M NauliPubMed 34901233
- Brain and behavior 11:e23002021Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingxiu Chang,Li Zhang,Huiyu Dai,Ling SunPubMed 34333865
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- Flow Cyt, IHC-Fr, IHC-P, IM, ICC/IF, ELISA
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Application ELISA | Reactivity Expected | Dilution info 1/10000 - 1/50000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Cited in 37 publications.
Key facts
- Description
- Goat Anti-Rat IgG H&L (FITC)
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- Flow Cyt, IHC-Fr, IHC-P, IM, ICC/IF, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
ab6840 was used at dilution 1/500 with the primary antibody Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker ab6326 in ICC/IF. See the review on ab6326.
Western blot - Goat Anti-Rat IgG H&L (FITC) (ab6840)
This data was developed using Anti-Cytokeratin 7 antibody [EP1620Y] ab68460, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Cytokeratin 7 antibody [EP1620Y] ab68460 observed at 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Cytokeratin 7 antibody [EP1620Y] ab68460 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. Anti-Cytokeratin 7 antibody [EP1620Y] ab68460 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2: KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (FITC) (ab6840) at 1/10000 dilution
Performed under reducing conditions.
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