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Suitable for Flow Cyt, WB, IHC-Fr, IHC-P, IM, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

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Images

Western blot - Goat Anti-Rat IgG H&L (FITC) preadsorbed (AB7093), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
Flow Cyt, WB, IHC-Fr, IHC-P, IM, ELISA, ICC/IF
Clonality
Polyclonal

Immunogen

  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759

Reactivity data

Application
Flow Cyt
Reactivity
Expected
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Expected
Dilution info
-
Notes

-

Application
IM
Reactivity
Expected
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Expected
Dilution info
1/10000 - 1/50000
Notes

-

Application
ICC/IF
Reactivity
Expected
Dilution info
1/1000 - 1/5000
Notes

-

Alternative names

Recommended products

Suitable for Flow Cyt, WB, IHC-Fr, IHC-P, IM, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

Key facts

Description
Goat Anti-Rat IgG H&L (FITC) preadsorbed
Host species
Goat
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
Flow Cyt, WB, IHC-Fr, IHC-P, IM, ELISA, ICC/IF
Clonality
Polyclonal
Pre-adsorbed
Yes
Immunogen
  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759
Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. 

Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Goat Anti-Rat IgG H&L (FITC) preadsorbed (ab7093), expandable thumbnail

    Western blot - Goat Anti-Rat IgG H&L (FITC) preadsorbed (ab7093)

    Western blot analysis of ab7093 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

    All lanes: Rat IgG at 50 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (FITC) preadsorbed (ab7093) at 1/1000 dilution

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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