Goat Anti-Rat IgG H&L (HRP)

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer : 5% NFDM/TBST.

The molecular weight is consistent with the benchmark : ab186733.

The positive cells were the consistent with the literature provided by licensor (PMID : 1843089).

All lanes:

Western blot - Anti-Argonaute-2 antibody [11A9] (<a href='/en-us/products/primary-antibodies/argonaute-2-antibody-11a9-ab252812'>ab252812</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

A549 (Human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Exposure time: 100s

• IP

Supplier Data

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (AB205720)

non-muscle Myosin IIB/MYH10 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate, 10µg with ab300647 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300647 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution. Lane 1 : HEK-293T whole cell lysate, 10µg Lane 2 : ab300647 IP in HEK-293T whole cell lysate Lane 3 : Rat monoclonal IgG2a (ab18450) instead of ab300647 in HEK-293T whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/non-muscle-myosin-iib-myh10-antibody-epr22564-23-rat-igg2a-chimeric-ab300647'>ab300647</a>) at 1/30 dilution

All lanes:

HEK-293T (human embryonic kidney epithelial cell)

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Observed band size: 250 kDa

false

Exposure time: 1s

• IP

Lab

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (AB205720)

VE Cadherin was immunoprecipitated from bEnd.3 whole cell lysate with ab91064 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab91064 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (<a href='/en-us/products/primary-antibodies/ve-cadherin-antibody-bv13-intercellular-junction-marker-ab91064'>ab91064</a>) at 1/50 dilution

Lane 1:

bEnd.3 whole cell lysate at 10 µg

Lane 2:

bEnd.3 whole cell lysate

Lane 3:

Rat IgG1- Isotype Control instead of <a href='/en-us/products/primary-antibodies/ve-cadherin-antibody-bv13-intercellular-junction-marker-ab91064'>ab91064</a> in bEnd.3 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/1000 dilution

Predicted band size: 90 kDa,120 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was produced using ab279291, the same clone in a different formulation.

The molecular weight observed is consistent with the literature (PMID : 824020, 2294, 6340792).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 3.25 seconds; Lane 2, 3 : 10 seconds.

All lanes:

Western blot - Anti-GFAP antibody [EPR1034Y] - Astrocyte marker - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/gfap-antibody-epr1034y-astrocyte-marker-rat-igg2a-chimeric-ab279291'>ab279291</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 49 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1,2 : 15 secs; Lane 3 : 3 secs

All lanes:

Western blot - Anti-LMO 4 antibody [20F8] (<a href='/en-us/products/primary-antibodies/lmo-4-antibody-20f8-ab243139'>ab243139</a>) at 1.354 µg/mL

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 3:

Mouse embryo tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

false

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

The expression profile observed is consistent with what has been described in the literature (PMID : 17389238).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (<a href='/en-us/products/primary-antibodies/pentraxin-3-ptx3-antibody-mnb1-ab90806'>ab90806</a>) at 1/1000 dilution

Lane 1:

Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 41 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using ab205720, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (<a href='/en-us/products/primary-antibodies/tubulin-antibody-yol1-34-loading-control-ab6161'>ab6161</a>) at 1 µg/mL

Lane 1:

Liver (Human) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

Liver (Rat) Tissue Lysate at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 6:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 36 kDa

Observed band size: 54 kDa

true

Exposure time: 15s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1,2 : 15 secs; Lane 3 : 3 secs

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243139).

All lanes:

Western blot - Anti-LMO 4 antibody [20F8] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/lmo-4-antibody-20f8-bsa-and-azide-free-ab255746'>ab255746</a>) at 1.354 µg/mL

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 3:

Mouse embryo tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was produced using the same clone in a different formulation, ab252845.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 30100605 and 24143218).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-NOTCH3 antibody [8G5] (<a href='/en-us/products/primary-antibodies/notch3-antibody-8g5-ab252845'>ab252845</a>) at 1/1000 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 244 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CBX1 / HP1 beta antibody [MAC353] (<a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Observed band size: 21 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

The expression profile weight observed is consistent with what has been described in the literature (PMID : 31634899).Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 seconds

All lanes:

Western blot - Anti-AMID antibody [6D8] (<a href='/en-us/products/primary-antibodies/amid-antibody-6d8-ab302673'>ab302673</a>) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell), whole cell lysate 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 µg

Lane 3:

A549 (human lu carcinoma epithelial cell), whole cell lysate 20 µg

Lane 4:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Observed band size: 40 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab252875).

All lanes:

Western blot - Anti-TRAF1 antibody [1F3] (<a href='/en-us/products/primary-antibodies/traf1-antibody-1f3-ab252875'>ab252875</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

Lane 2:

THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was produced using ab279300, the same clone in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD20 antibody [EP459Y] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cd20-antibody-ep459y-rat-igg2a-chimeric-ab279300'>ab279300</a>) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 2:

Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 33 kDa

false

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22745433 and 23071310)

Exposure time : 10 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252853).

All lanes:

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S7) antibody [4E12] (<a href='/en-us/products/primary-antibodies/rna-polymerase-ii-ctd-repeat-ysptsps-phospho-s7-antibody-4e12-ab252853'>ab252853</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (Untreated membrane) at 10 µg

Lane 2:

HeLa whole cell lysate (Phosphatase treated membrane) at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Lanes 1 - 2:

Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution

Predicted band size: 217 kDa

Observed band size: 260 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was produced using ab279294, the same clone in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PD-L1 antibody [CAL10] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/pd-l1-antibody-cal10-rat-igg2a-chimeric-ab279294'>ab279294</a>) at 1/1000 dilution

Lane 1:

CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate at 20 µg

Lane 2:

CHO-PD-L1 (PD-L1 stably expressed Chinese hamster ovary epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human placenta tissue lysate at 20 µg

Lane 4:

NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 40-60 kDa

false

Exposure time: 8s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

All lanes:

Western blot - Anti-IDH1 antibody [RcMab-1] (<a href='/en-us/products/primary-antibodies/idh1-antibody-rcmab-1-ab256557'>ab256557</a>) at 1.479 µg/mL

Lane 1:

Wild type HAP1 whole cell lysate at 20 µg

Lane 2:

IDH1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 46 kDa

false

Exposure time: 8s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1/3 : 3 seconds; Lane 2 : 15 seconds; Lane 4 : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252878).

All lanes:

Western blot - Anti-WDR12 antibody [1B8] (<a href='/en-us/products/primary-antibodies/wdr12-antibody-1b8-ab252878'>ab252878</a>) at 1.235 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 47 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 : 37 secs; Lanes 4-5 : 5 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252816).

All lanes:

Western blot - Anti-beta Amyloid antibody [2E9] (<a href='/en-us/products/primary-antibodies/beta-amyloid-antibody-2e9-ab252816'>ab252816</a>) at 0.583 µg/mL

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

Human brain tissue lysate at 10 µg

Lane 5:

Human hippocampus tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 87 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-4 : 1 sec; Lane 5 : 5 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252849).

All lanes:

Western blot - Anti-Pescadillo antibody [8E9] (<a href='/en-us/products/primary-antibodies/pescadillo-antibody-8e9-ab252849'>ab252849</a>) at 0.489 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

Predicted band size: 68 kDa

false

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : lane 1, 2 : 3 minutes lane 3-5 : 59 seconds

All lanes:

Western blot - Anti-p75 NGF Receptor antibody [NORI138B] (<a href='/en-us/products/primary-antibodies/p75-ngf-receptor-antibody-nori138b-ab271289'>ab271289</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 45 kDa

Observed band size: 65 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22715410).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241544).

All lanes:

Western blot - Anti-SA1 antibody [SUSI63B] (<a href='/en-us/products/primary-antibodies/sa1-antibody-susi63b-ab241544'>ab241544</a>) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Lane 2:

MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 10 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 5:

TF-1 (human Erythroleukemia erythroblast), whole cell lysate at 10 µg

Lane 6:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 144 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was developed using ab90806, the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1, 2 : 15 seconds; Lane 3 : 3 seconds; Lane 4 : 180 seconds.

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/pentraxin-3-ptx3-antibody-mnb1-bsa-and-azide-free-ab264487'>ab264487</a>) at 1/1000 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Mouse placenta tissue lysate at 20 µg

Lane 3:

Rat placenta tissue lysate at 20 µg

Lane 4:

3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 41 kDa

false

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was developed using ab90806, the same antibody clone in a different buffer formulation.

The expression profile observed is consistent with what has been described in the literature (PMID : 17389238).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/pentraxin-3-ptx3-antibody-mnb1-bsa-and-azide-free-ab264487'>ab264487</a>) at 1/1000 dilution

Lane 1:

Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 41 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Samples are non-boiled as boiling may cause protein aggregates.

Exposure times : Lane 1 : 15 secs; Lane 2 : 92 secs.

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252814).

All lanes:

Western blot - Anti-Amyloid Precursor Protein antibody [14D6] (<a href='/en-us/products/primary-antibodies/amyloid-precursor-protein-antibody-14d6-ab252814'>ab252814</a>) at 2.308 µg/mL

Lane 1:

Human hippocampus tissue lysate at 40 µg

Lane 2:

Rat hippocampus tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 86 kDa

Observed band size: 95 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 5 secs.

The negative control and the molecular weight of HCN1 observed is consistent with what has been described in the literature (PMID : 12786975).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252835).

All lanes:

Western blot - Anti-HCN1 antibody [7C3] (<a href='/en-us/products/primary-antibodies/hcn1-antibody-7c3-ab252835'>ab252835</a>) at 0.326 µg/mL

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 99 kDa

false

Exposure time: 5s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ab181602 was used as a GAPDH loading control at a 1/200000 dilution.

In Western blot, anti-CBX1 antibody ab10811 was shown to bind specifically to CBX1. Target of interest was observed at 21kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in CBX1 knockout cell line (lane 2).

All lanes:

Western blot - Anti-CBX1 / HP1 beta antibody [MAC353] (<a href='/en-us/products/primary-antibodies/cbx1-hp1-beta-antibody-mac353-ab10811'>ab10811</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

CBX1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Observed band size: 21 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : SW480 and colo 205 (PMID : 31000598). Samples are non-boiled as boiling may cause protein aggregation. The expression profile observed is consistent with what has been described in the literature ( PMID : 32188872). Exposure time : 26 seconds

All lanes:

Western blot - Anti-xCT antibody [3A12] (<a href='/en-us/products/primary-antibodies/xct-antibody-3a12-ab300667'>ab300667</a>) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

COLO 205 (human colon; derived from metastatic site: ascites epithelial), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Observed band size: 55 kDa,35 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

The expression profile observed is consistent with what has been described in the literature (PMID : 19079332)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-GBP1 antibody [1B1] (<a href='/en-us/products/primary-antibodies/gbp1-antibody-1b1-ab252832'>ab252832</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HeLa treated with 100U/ml IFN gamma (<a href='/en-us/products/proteins-peptides/recombinant-human-interferon-gamma-protein-active-ab9659'>ab9659</a>) for 24 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 68 kDa

Observed band size: 67 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252838).

All lanes:

Western blot - Anti-ING5 antibody [7A11] (<a href='/en-us/products/primary-antibodies/ing5-antibody-7a11-ab252838'>ab252838</a>) at 0.66 µg/mL

Lane 1:

Jurkat cytoplasmic fraction at 10 µg

Lane 2:

Jurkat nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was produced using the same clone but in a different buffer, ab243140.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Bax antibody [21C10] (<a href='/en-us/products/primary-antibodies/bax-antibody-21c10-ab243140'>ab243140</a>) at 1/5000 dilution

Lane 1:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate at 20 µg

Lane 2:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 21 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

The expression molecular weight observed is consistent with what has been described in the literature (PMID : 16785505).

Blocking/diluting buffer and concentration : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243149).

All lanes:

Western blot - Anti-TCF12 antibody [7B3] (<a href='/en-us/products/primary-antibodies/tcf12-antibody-7b3-ab243149'>ab243149</a>) at 0.394 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 5:

WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 72 kDa

Observed band size: 92 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

All lanes:

Western blot - Anti-SI-CLP antibody [1B8] (<a href='/en-us/products/primary-antibodies/si-clp-antibody-1b8-ab305176'>ab305176</a>) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 20 µg

Lane 2:

Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

Lane 4:

U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

Lane 5:

Mouse pancreas lysate at 20 µg

Lane 6:

Mouse testis lysate at 20 µg

Lane 7:

A20 (mouse reticum sarcoma B lymphocyte), whole cell lysate 20 at 20 µg

Lane 8:

EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

Lane 9:

EL4.IL-2 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Observed band size: 44 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 mins.

The expression molecular weight observed is consistent with what has been described in the literature (PMID : 19663701).

This blot was developed using a higher sensitivity ECL substrate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

All lanes:

Western blot - Anti-LST1 antibody [7E2] (<a href='/en-us/products/primary-antibodies/lst1-antibody-7e2-ab252839'>ab252839</a>) at 0.432 µg/mL

All lanes:

HEK-293 (human embryonic kidney epithelial cell) lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

Predicted band size: 11 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 15 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252829).

All lanes:

Western blot - Anti-EWSR1/EWS antibody [21B1] (<a href='/en-us/products/primary-antibodies/ewsr1-ews-antibody-21b1-ab252829'>ab252829</a>) at 0.361 µg/mL

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 68 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

This image was produced using the same antibody clone but in a different formulation ab252825, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-Dnmt1 antibody [2C1] (<a href='/en-us/products/primary-antibodies/dnmt1-antibody-2c1-ab252825'>ab252825</a>) at 0.353 µg/mL

Lane 1:

NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

Predicted band size: 183 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : K562 (PMID : 27217538).

Exposure time : 59 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252813).

All lanes:

Western blot - Anti-AICDA antibody [5G9] (<a href='/en-us/products/primary-antibodies/aicda-antibody-5g9-ab252813'>ab252813</a>) at 1.264 µg/mL

Lane 1:

NAMALWA (human Burkitts lymphoma B lymphocyte), whole cell lysate at 10 µg

Lane 2:

Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 10 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 4:

WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

All lanes:

Western blot - Anti-SI-CLP antibody [1B8] (<a href='/en-us/products/primary-antibodies/si-clp-antibody-1b8-ab305176'>ab305176</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

THP-1 transfected with siRNA specifically targeti SI-CLP, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Observed band size: 44 kDa

false

Exposure time: 62s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking buffer : 5% NFDM/TBST

Exposure time : 5 seconds

All lanes:

Western blot - Anti-RPA32/RPA2 antibody [4E4] (<a href='/en-us/products/primary-antibodies/rpa32-rpa2-antibody-4e4-ab252861'>ab252861</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab50610).

Blocking/Dilution buffer : 5% NFDM.

The molecular weight observed is consistent with what has been described in the literature (PMID : 17442773).

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-NUP98 antibody [2H10] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/nup98-antibody-2h10-bsa-and-azide-free-ab252256'>ab252256</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/50000 dilution

Predicted band size: 198 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11893590 and PMID : 10642500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252868).

All lanes:

Western blot - Anti-Serum Response Factor SRF antibody [2C5] (<a href='/en-us/products/primary-antibodies/serum-response-factor-srf-antibody-2c5-ab252868'>ab252868</a>) at 1.082 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 4:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa,57 kDa,67 kDa

false

Exposure time: 20s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 : 5 secs; Lanes 4/5/7 : 3 secs; Lane 6 : 15 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab243142).

All lanes:

Western blot - Anti-MLKL antibody [3H1] (<a href='/en-us/products/primary-antibodies/mlkl-antibody-3h1-ab243142'>ab243142</a>) at 1/2000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell), whole cell lysate at 10 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 7:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution

Predicted band size: 54 kDa

false