Goat Anti-Rat IgG H&L (HRP)

40 product images

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking buffer: 5% NFDM/TBST.
  

  The molecular weight observed was in consistent with the literature (PMID:25512468) and the benchmark Anti-RBPJK antibody [EPR13479] ab180588.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RBPJK antibody [7A11] ab252858).

  All lanes: Western blot - Anti-RBPJK antibody [7A11] (Anti-RBPJK antibody [7A11] ab252858) at 1/1000 dilution

  Lane 1: Daudi (human Burkitt’s lymphoma lymphoblast), whole cell lysate at 20 µg

  Lane 2: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 3: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 4: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 56 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ING5 antibody [7A11] ab252838).

  All lanes: Western blot - Anti-ING5 antibody [7A11] (Anti-ING5 antibody [7A11] ab252838) at 0.66 µg/mL

  Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 2: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

  Lane 3: A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 4: HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 10 µg

  Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 6: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 28 kDa

  Observed band size: 28 kDa

  Exposure time: 3s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (Anti-TRAF1 antibody [1F3] ab252875).

  All lanes: Western blot - Anti-TRAF1 antibody [1F3] (Anti-TRAF1 antibody [1F3] ab252875) at 1/1000 dilution

  Lane 1: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

  Lane 2: THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 46 kDa

  Observed band size: 50 kDa

  Exposure time: 3min

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  The expression profile observed is consistent with what has been described in the literature (PMID:19079332)
  

  Blocking/diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-GBP1 antibody [1B1] (Anti-GBP1 antibody [1B1] ab252832) at 1/1000 dilution

  Lane 1: Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 2: HeLa treated with 100U/ml IFN gamma (Recombinant human Interferon gamma protein (Active) ab9659) for 24 hours, whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 68 kDa

  Observed band size: 67 kDa

  Exposure time: 3s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  Exposure time: Lanes 1/3: 3 seconds; Lane 2 : 15 seconds; Lane 4: 1 second.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WDR12 antibody [1B8] ab252878).

  All lanes: Western blot - Anti-WDR12 antibody [1B8] (Anti-WDR12 antibody [1B8] ab252878) at 1.235 µg/mL

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 2: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

  Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

  Lane 4: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 48 kDa

  Observed band size: 47 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was produced using the same clone in a different formulation, Anti-NOTCH3 antibody [8G5] ab252845.
  

  The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 30100605 and 24143218).
  Negative control: MDA-MB-231.

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Exposure times: Lane 1: 70 seconds; Lanes 2-3: 3 minutes.

  All lanes: Western blot - Anti-NOTCH3 antibody [8G5] (Anti-NOTCH3 antibody [8G5] ab252845) at 1/1000 dilution

  Lane 1: T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 10 µg

  Lane 2: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 244 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was produced using the same clone but in a different formulation, Anti-BOP1 antibody [6H11] ab252819.
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Exposure times: Lane 1: 10 seconds; Lane 2: 59 seconds; Lane 3: 6 seconds.

  All lanes: Western blot - Anti-BOP1 antibody [6H11] (Anti-BOP1 antibody [6H11] ab252819) at 1/5000 dilution

  Lane 1: Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 84 kDa

  Observed band size: 110 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  Negative control: Raji (PMID:11313386; PMID:11054674).

  Exposure time: lane 1, 2:10 seconds lane 3, 4: 37 seconds

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SH2D1A/SAP antibody [1D12] ab252866).

  All lanes: Western blot - Anti-SH2D1A/SAP antibody [1D12] (Anti-SH2D1A/SAP antibody [1D12] ab252866) at 1/1000 dilution

  Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

  Lane 2: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

  Lane 3: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 4: HDLM-2 (human Hodgkin lymphoma), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 14 kDa

  Observed band size: 14 kDa, 40 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking and dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Firefly Luciferase antibody [LAS126A] (Anti-Firefly Luciferase antibody [LAS126A] ab241541) at 1/5000 dilution

  Lane 1: Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

  Lane 2: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/20000 dilution

  Predicted band size: 61 kDa

  Observed band size: 61 kDa

  Exposure time: 1s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glutathione Peroxidase 4 antibody [1B4] ab252833).

  All lanes: Western blot - Anti-Glutathione Peroxidase 4 antibody [1B4] (Anti-Glutathione Peroxidase 4 antibody [1B4] ab252833) at 0.976 µg/mL

  Lane 1: Mouse testis tissue lysate at 20 µg

  Lane 2: Rat testis tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 22 kDa

  Observed band size: 17 kDa

  Exposure time: 1s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-p75 NGF Receptor antibody [NORI146C] ab271290).

  All lanes: Western blot - Anti-p75 NGF Receptor antibody [NORI146C] (Anti-p75 NGF Receptor antibody [NORI146C] ab271290) at 0.755 µg/mL

  All lanes: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 45 kDa

  Exposure time: 59s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Samples are non-boiled as boiling may cause protein aggregates.
  

  Exposure times: Lane 1: 15 secs; Lane 2: 92 secs.

  Blocking/Dilution buffer: 5% NFDM/TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Amyloid Precursor Protein antibody [14D6] ab252814).

  All lanes: Western blot - Anti-Amyloid Precursor Protein antibody [14D6] (Anti-Amyloid Precursor Protein antibody [14D6] ab252814) at 2.308 µg/mL

  Lane 1: Human hippocampus tissue lysate at 40 µg

  Lane 2: Rat hippocampus tissue lysate at 40 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 86 kDa

  Observed band size: 95 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was produced using the same clone but in a different formulation, Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] ab252851.
  

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22745433 and 23071310).

  This blot was developed using a higher sensitivity ECL substrate.
  This antibody reacts with rat on WB.

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] ab252851) at 1/1000 dilution

  Lane 1: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate (Untreated membrane) at 10 µg

  Lane 2: PC-12 whole cell lysate (Phosphatase treated membrane) at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 217 kDa

  Observed band size: 260 kDa

  Exposure time: 3min

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Granulin is a glycoprotein, the molecular weight observed is consistent with what has been described in the literature (PMID: 8496151).
  

  Exposure times: Lanes 1-2/4: 37 seconds; Lanes 3: 5 seconds.

  Blocking/Dilution buffer: 5% NFDM/TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Granulin antibody [8H10] ab252834).

  All lanes: Western blot - Anti-Granulin antibody [8H10] (Anti-Granulin antibody [8H10] ab252834) at 1.14 µg/mL

  Lane 1: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 10 µg

  Lane 2: 4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 3: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

  Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 64 kDa

  Observed band size: 75 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (Anti-NUP98 antibody [2H10] - Nuclear Pore Marker ab50610).
  

  Blocking/Dilution buffer: 5% NFDM.

  The molecular weight observed is consistent with what has been described in the literature (PMID: 17442773).

  This blot was developed using a higher sensitivity ECL substrate.

  All lanes: Western blot - Anti-NUP98 antibody [2H10] - BSA and Azide free (Anti-NUP98 antibody [2H10] - BSA and Azide free ab252256) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/50000 dilution

  Predicted band size: 198 kDa

  Exposure time: 3min

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure time: 103 secs.

  All lanes: Western blot - Anti-p53 antibody [POE316A] (Anti-p53 antibody [POE316A] ab241566) at 1/1000 dilution

  Lane 1: LLC1 (mouse lung carcinoma), whole cell lysate at 20 µg

  Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 43 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure times: Lanes 1-3: 5 secs; Lanes 4/5/7: 3 secs; Lane 6: 15 secs.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab243142).

  All lanes: Western blot - Anti-MLKL antibody [3H1] (Anti-MLKL antibody [3H1] ab243142) at 1/2000 dilution

  Lane 1: HUVEC (human umbilical vein endothelial cell), whole cell lysate at 10 µg

  Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

  Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

  Lane 6: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

  Lane 7: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution

  Predicted band size: 54 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure times: Lane 1,2: 15 secs; Lane 3: 3 secs

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LMO 4 antibody [20F8] ab243139).

  All lanes: Western blot - Anti-LMO 4 antibody [20F8] - BSA and Azide free (Anti-LMO 4 antibody [20F8] - BSA and Azide free ab255746) at 1.354 µg/mL

  Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

  Lane 2: T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

  Lane 3: Mouse embryo tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IDH1 antibody [RcMab-1] ab256557).

  All lanes: Western blot - Anti-IDH1 antibody [RcMab-1] (Anti-IDH1 antibody [RcMab-1] ab256557) at 1.479 µg/mL

  Lane 1: Wild type HAP1 whole cell lysate at 20 µg

  Lane 2: IDH1 knockout HAP1 whole cell lysate at 20 µg

  Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 4: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 5: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 46 kDa

  Exposure time: 8s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using Anti-PAX9 antibody [7C2] ab28538, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  Exposure time: 70 seconds

  All lanes: Western blot - Anti-PAX9 antibody [7C2] - BSA and Azide free (Anti-PAX9 antibody [7C2] - BSA and Azide free ab255977) at 1/1000 dilution

  Lane 1: Mouse esophagus tissue lysate at 20 µg

  Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 36 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking buffer: 5% NFDM/TBST

  All lanes: Western blot - Anti-RPA32/RPA2 antibody [4E4] (Anti-RPA32/RPA2 antibody [4E4] ab252861) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

  Lane 2: HUVEC (human umbilical vein endothelial cell ), whole cell lysate at 20 µg

  Lane 3: U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg

  Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

  Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 29 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-PAX9 antibody [7C2] (Anti-PAX9 antibody [7C2] ab28538) at 1/1000 dilution

  Lane 1: Mouse esophagus tissue lysate at 20 µg

  Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 36 kDa

  Exposure time: 70s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using Anti-PD-L2 antibody [CAL28] - Rat IgG2a (Chimeric) ab283347 the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer: 5% NFDM/TBST

  Exposure time: 10 seconds

  Negative control: Jurkat. (PMID: 27631416).

  All lanes: Western blot - Anti-PD-L2 antibody [CAL28] - Rat IgG2a (Chimeric) (Anti-PD-L2 antibody [CAL28] - Rat IgG2a (Chimeric) ab283347) at 1/1000 dilution

  Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

  Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 31 kDa

  Observed band size: 45-60 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-Oct-2 antibody [9A2] ab243153).
  

  The molecular weight observed is consistent with what has been described in the literature (PMID: 3265124).

  Exposure times: Lanes 1-3: 3 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lane 6: 44 seconds.

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Oct-2 antibody [9A2] (Anti-Oct-2 antibody [9A2] ab243153) at 1/1000 dilution

  Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 2: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 3: Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

  Lane 4: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg

  Lane 5: Human tonsil tissue lysate at 20 µg

  Lane 6: Mouse spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 51 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure time: 10 secs.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MCL1 antibody [19C4-15] ab243136).

  All lanes: Western blot - Anti-MCL1 antibody [19C4-15] (Anti-MCL1 antibody [19C4-15] ab243136) at 1/1000 dilution

  Lane 1: SK-MEL-2 (human skin malignant melanoma) whole cell lysate at 20 µg

  Lane 2: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

  Lane 3: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

  Lane 4: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

  Predicted band size: 37 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BACH2 antibody [7A4] ab243148).
  

  Blocking/diluting buffer and concentration: 5% NFDM/TBST.

  Exposure time:

  Lane 1: 3 minutes
  Lanes 2-5: 3 seconds
  Lane 6: 5 seconds

  Negative control: THP-1 and HL-60 (PMID: 10949928).

  The molecular weight observed is consistent with what has been described in the literature (PMID: 9755173).

  All lanes: Western blot - Anti-BACH2 antibody [7A4] (Anti-BACH2 antibody [7A4] ab243148) at 1/2000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

  Lane 2: Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 3: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

  Lane 4: HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate at 20 µg

  Lane 5: NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 6: WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 93 kDa

  Observed band size: 130 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-TVA antibody [PICA187E] (Anti-TVA antibody [PICA187E] ab271292) at 1/5000 dilution

  Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

  Lane 2: HEK-293T transfected with TVA expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/50000 dilution

  Observed band size: 25-37 kDa

  Exposure time: 10s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This blot was developed using a higher sensitivity ECL substrate.
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PRDM1/Blimp1 antibody [6D3] ab243146).

  All lanes: Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (Anti-PRDM1/Blimp1 antibody [6D3] ab243146) at 0.89 µg/mL

  Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

  Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 32 kDa, 91 kDa

  Observed band size: 95 kDa

  Exposure time: 3min

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  The expression profile observed is consistent with what has been described in the literature (PMID:17389238).
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806) at 1/1000 dilution

  Lane 1: Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

  Lane 2: HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 41 kDa

  Exposure time: 15s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure times: Lane 1: 26 seconds; Lane 2: 3 minutes.

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Serum Response Factor SRF antibody [2C5] ab252868).

  All lanes: Western blot - Anti-Serum Response Factor SRF antibody [2C5] (Anti-Serum Response Factor SRF antibody [2C5] ab252868) at 0.082 µg/mL

  Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

  Lane 2: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 52 kDa

  Observed band size: 52 kDa, 57 kDa, 67 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-Tubulin antibody [YOL1/34] - Loading Control ab6161 overnight at 4°C. Antibody binding was detected using ab205720, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (Anti-Tubulin antibody [YOL1/34] - Loading Control ab6161) at 1 µg/mL

  Lane 1: Liver (Human) Tissue Lysate at 10 µg

  Lane 2: Liver (Mouse) Tissue Lysate at 10 µg

  Lane 3: Liver (Rat) Tissue Lysate at 10 µg

  Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 36 kDa

  Observed band size: 54 kDa

  Exposure time: 15s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  IHC image of tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 at 2ug/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature.

  An HRP-conjugated secondary (ab205720, 1/2000 dilution) was used for 1hr at room temperature.

  The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Exposure time:

  Lane 1,2,5:3 seconds

  Lane 3,4:10 seconds

  Blocking buffer / Diluting buffer and concentration: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Bcl-2 antibody [NOR 235J] (Anti-Bcl-2 antibody [NOR 235J] ab241548) at 1/1000 dilution

  Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

  Lane 2: U937 (human histiocytic lymphoma monocyte), Whole cell lysate at 20 µg

  Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 4: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 5: Human tonsil tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 26 kDa

  Observed band size: 26 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using Anti-EBNA2 antibody [R3] ab252828, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:8383171).

  Exposure time: 10 seconds

  All lanes: Western blot - Anti-EBNA2 antibody [R3] (Anti-EBNA2 antibody [R3] ab252828) at 1/1000 dilution

  Lane 1: Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg

  Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 53 kDa

  Observed band size: 75 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  The double bands observed in lane 5 is consistent with what has been described in the literatures (PMID: 29107940, 17620360).

  Exposure time: lane 1-3: 6 seconds; lane 4-5: 3 seconds.

  This image was produced using the same antibody clone but in a different formulation Anti-TPN antibody [7F6] ab252869, PBS, sodium azide, glycerol and BSA.

  All lanes: Western blot - Anti-TPN antibody [7F6] (Anti-TPN antibody [7F6] ab252869) at 1/1000 dilution

  Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 2: HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg

  Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

  Lane 4: Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate at 20 µg

  Lane 5: Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 48 kDa

  Observed band size: 47 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  Blocking and diluting buffer: 5% NFDM/TBST
  

  Negative control: MCF7 (HPA database)

  Exposure time: 37 seconds

  All lanes: Western blot - Anti-Iba1 antibody [EPR16589] - Rat IgG2a (Chimeric) (Anti-Iba1 antibody [EPR16589] - Rat IgG2a (Chimeric) ab283346) at 1/1000 dilution

  Lane 1: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

  Lane 2: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 16 kDa

  Observed band size: 16 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using Anti-Bak antibody [7D10] ab243151, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12881569).

  This antibody reacts with an unidentifiable protein around 150kDa.

  Exposure time: 1 second.

  All lanes: Western blot - Anti-Bak antibody [7D10] (Anti-Bak antibody [7D10] ab243151) at 1/5000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 2: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

  Lane 3: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

  Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

  Lane 5: T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 10 µg

  Lane 6: Human heart tissue lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 23 kDa

  Observed band size: 20 kDa, 25 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using 282277, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  Lysates were made freshly and used in WB immediately to minimize protein degradation.

  The molecular weight observed is consistent with what has been described in the literature (PMID: 32732889)

  Negative control: C2C12.

  Exposure time: 1 second

  All lanes: Western blot - Anti-VE Cadherin antibody [11D4.1] (Anti-VE Cadherin antibody [11D4.1] ab282277) at 1/1000 dilution

  Lane 1: bEnd.3 (mouse brain endothelioma), whole cell lysate at 20 µg

  Lane 2: C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 88 kDa

  Observed band size: 125 kDa, 90 kDa

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using Anti-Pentraxin 3/PTX3 antibody [MNB1] ab90806, the same antibody clone in a different buffer formulation.
  

  The expression profile observed is consistent with what has been described in the literature (PMID:17389238).

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free ab264487) at 1/1000 dilution

  Lane 1: Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

  Lane 2: HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

  Predicted band size: 41 kDa

  Exposure time: 15s

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720)

  This data was developed using Anti-DLL1 antibody [1F9] ab252824, the same antibody clone but in a different formulation.
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17664336, 21985982).

  Low expression: mouse heart (PMID: 7671806).

  All lanes: Western blot - Anti-DLL1 antibody [1F9] (Anti-DLL1 antibody [1F9] ab252824) at 1/1000 dilution

  Lane 1: Mouse lung tissue lysate at 20 µg

  Lane 2: Mouse heart tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

  Predicted band size: 78 kDa

  Exposure time: 5s