Goat Anti-Rat IgG H&L (HRP)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (AB97057)

IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97057, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Unknown

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB97057)

Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

All lanes:

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (<a href='/en-us/products/primary-antibodies/tubulin-antibody-yl1-2-loading-control-ab6160'>ab6160</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/2000 dilution

Lanes 3 - 4:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution

Lanes 5 - 6:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 52 kDa

true

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB97057)

SDS Page 10-20% gradient gel.

All lanes:

Western blot - Anti-IZUMO1 antibody [125] (<a href='/en-us/products/primary-antibodies/izumo1-antibody-125-bsa-and-azide-free-ab211626'>ab211626</a>) at 1/1000 dilution

Lane 1:

mouse testis lysate

Lane 2:

mouse sperm lysate

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution

Predicted band size: 39 kDa

false

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB97057)

The main band is as expected at 80 kDa since the target is heavily glycosylated and phosphorylated.

All lanes:

Western blot - Anti-CD34 antibody [MEC 14.7] (<a href='/en-us/products/primary-antibodies/cd34-antibody-mec-147-ab8158'>ab8158</a>) at 1 µg/mL

All lanes:

Mouse Lung Membrane Tissue Lysate (ab171830) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/20000 dilution

Predicted band size: 40 kDa

Observed band size: 80 kDa

false

Exposure time: 2min

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB97057)

All lanes:

Western blot - Anti-IZUMO1 antibody [125] (<a href='/en-us/products/primary-antibodies/izumo1-antibody-125-bsa-and-azide-free-ab211626'>ab211626</a>) at 1/100 dilution

Lane 1:

human testis lysate

Lane 2:

human sperm lysate

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution

Predicted band size: 39 kDa

false