Goat anti-rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot and ELISA.
- Cited in over 140 publications
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- Biomolecules 14:2024Diverse Response to Local Pharmacological Blockade of Sirt1 Cleavage in Age-Induced versus Trauma-Induced Osteoarthritis Female Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonathan H Maatuf,Miya Marco,Shani Unger-Gelman,Eli Farhat,Anna Zobrab,Ankita Roy,Ashish Kumar,Idan Carmon,Eli Reich,Mona Dvir-GinzbergPubMed 38254681
- International journal of molecular sciences 24:2023Sex Drives Functional Changes in the Progression and Regression of Liver Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatia Sayaf,Ilaria Zanotto,Daniela Gabbia,Dafne Alberti,Giulia Pasqual,Alice Zaramella,Alberto Fantin,Sara De Martin,Francesco Paolo RussoPubMed 38003640
- American journal of cancer research 13:4918-49302023AZD7648, a DNA-PKcs inhibitor, overcomes radioresistance in Hep3B xenografts and cells under tumor hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJimin Ha,Mijeong Park,Yuri Lee,Sang Hyun Choi,Byoung Soo Kim,Hunjoo Ha,Youn Kyoung JeongPubMed 37970336
- Retrovirology 20:152023Primate TRIM34 is a broadly-acting, TRIM5-dependent lentiviral restriction factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoy Twentyman,Anthony Khalifeh,Abby L Felton,Michael Emerman,Molly OhainlePubMed 37608289
- International journal of molecular sciences 24:2023Suppression of Indoxyl Sulfate Accumulation Reduces Renal Fibrosis in Sulfotransferase 1a1-Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuixian Hou,Mai Horikawa,Yuki Narita,Hirofumi Jono,Yutaka Kakizoe,Yuichiro Izumi,Takashige Kuwabara,Masashi Mukoyama,Hideyuki SaitoPubMed 37511089
- World journal of gastroenterology 29:3084-31022023Metronomic capecitabine inhibits liver transplant rejection in rats by triggering recipients' T cell ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Wang,Zheng-Lu Wang,Sai Zhang,De-Jun Kong,Rui-Ning Yang,Lei Cao,Jian-Xi Wang,Sei Yoshida,Zhuo-Lun Song,Tao Liu,Shun-Li Fan,Jia-Shu Ren,Jiang-Hong Li,Zhong-Yang Shen,Hong ZhengPubMed 37346150
- Cells 12:2023A Novel Small Promoter Enhances AAV-Mediated Gene Therapy in Mouse Models of Niemann-Pick Type C1 Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Paul Hughes,Hemanth Ramesh Nelvagal,Oliver Coombe-Tennant,Dave Smith,Claire Smith,Giulia Massaro,Laura Poupon-Bejuit,Frances Mary Platt,Ahad Abdul RahimPubMed 37371089
- PLoS pathogens 19:e10114302023An essential role for an Fe-S cluster protein in the cytochrome c oxidase complex of Toxoplasma parasites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel A Leonard,Yuan Tian,Feng Tan,Giel G van Dooren,Jenni A HaywardPubMed 37262100
- Nature 618:402-4102023Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHector Foronda,Yangxue Fu,Adriana Covarrubias-Pinto,Hartmut T Bocker,Alexis González,Eric Seemann,Patricia Franzka,Andrea Bock,Ramachandra M Bhaskara,Lutz Liebmann,Marina E Hoffmann,Istvan Katona,Nicole Koch,Joachim Weis,Ingo Kurth,Joseph G Gleeson,Fulvio Reggiori,Gerhard Hummer,Michael M Kessels,Britta Qualmann,Muriel Mari,Ivan Dikić,Christian A HübnerPubMed 37225994
- NPJ vaccines 8:742023Developmental and reproductive toxicity of a recombinant protein subunit COVID-19 vaccine (ZF2001) in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYisheng Song,Jinjin Shao,Guangbiao She,Wanqiang Lv,Guoyu Chen,Jing Liu,Lili Zhang,Chengda Zhang,Jiahong Wang,Ruiyu Tian,Lianpan Dai,George F Gao,Enqi Huang,Lijiang ZhangPubMed 37225729
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/5000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (as primary) |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/20000.00000 | Notes Colorimetric: Use 1/5000-1/30000, Chemiluminescent: use 1/10000-1/50000 |
Alternative names
Ig gamma-1 chain C region
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Goat anti-rat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot and ELISA.
- Cited in over 140 publications
Key facts
- Description
- Goat Anti-Rat IgG H&L (HRP)
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
Part of the AbExcel range.
Product promise
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab97057)
IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97057, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057)
SDS Page 10-20% gradient gel.
All lanes: Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (Anti-IZUMO1 antibody [125] - BSA and Azide free ab211626) at 1/1000 dilution
Lane 1: mouse testis lysate
Lane 2: mouse sperm lysate
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution
Predicted band size: 39 kDa
Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057)
All lanes: Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (Anti-IZUMO1 antibody [125] - BSA and Azide free ab211626) at 1/100 dilution
Lane 1: human testis lysate
Lane 2: human sperm lysate
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution
Predicted band size: 39 kDa
Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057)
The main band is as expected at 80 kDa since the target is heavily glycosylated and phosphorylated.
All lanes: Western blot - Anti-CD34 antibody [MEC 14.7] (Anti-CD34 antibody [MEC 14.7] ab8158) at 1 µg/mL
All lanes: Mouse Lung Membrane Tissue Lysate (ab171830) at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 80 kDa
Exposure time: 2min
Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057)
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
SecondaryLanes 1 - 2: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/2000 dilution
Lanes 3 - 4: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution
Lanes 5 - 6: Western blot - Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 52 kDa
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