Suitable for IHC-P, Ie. Cited in 8 publications.
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- Frontiers in immunology 13:10177802022Attenuated Salmonella potentiate PD-L1 blockade immunotherapy in a preclinical model of colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBesan H Al-Saafeen,Ashraf Al-Sbiei,Ghada Bashir,Yassir A Mohamed,Razan J Masad,Maria J Fernandez-Cabezudo,Basel K Al-RamadiPubMed 36605208
- Biomedicines 10:2022Combination of Dexamethasone and Tofacitinib Reduces Xenogeneic MSC-Induced Immune Responses in a Mouse Model of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Kyung Lee,Su Hyeon Myeong,Jung Won Hwang,Jason K Sa,Hyo Jin Son,Hee Jin Kim,Hyemin Jang,Jong Wook Chang,Duk L NaPubMed 36009433
- Frontiers in pharmacology 13:6638552022Mitigation of Ionizing Radiation-Induced Gastrointestinal Damage by Insulin-Like Growth Factor-1 in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaroslav Pejchal,Ales Tichy,Adela Kmochova,Lenka Fikejzlova,Klara Kubelkova,Marcela Milanova,Anna Lierova,Alzbeta Filipova,Lubica Muckova,Jana CizkovaPubMed 35847048
- Disease models & mechanisms 15:2022An enriched environment re-establishes metabolic homeostasis by reducing obesity-induced inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSol Díaz de León-Guerrero,Jonathan Salazar-León,Karla F Meza-Sosa,David Valle-Garcia,Diana Aguilar-León,Gustavo Pedraza-Alva,Leonor Pérez-MartínezPubMed 35112705
- OncoTargets and therapy 14:3373-33812021Efficacy and Safety of Systemic and Locoregional Cisplatin Chronotherapy in Rats with Ovarian Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGalina Kireeva,Ekaterina Gubareva,Mikhail Maydin,Vladislav Osetnik,Stepan Kruglov,Andrey Panchenko,Anastasia Dorofeeva,Margarita Tyndyk,Elena Fedoros,Vladimir AnisimovPubMed 34079283
- Nature communications 11:63642020Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingyan Li et. al.PubMed 33311474
- Molecules (Basel, Switzerland) 25:2020Modeling of Chemoperfusion vs. Intravenous Administration of Cisplatin in Wistar Rats: Adsorption and Tissue Distribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGalina Kireeva et. al.PubMed 33076418
- Clinical cancer research : an official journal of 24:2574-25842018Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroshi Nakashima et. al.PubMed 29511029
Key facts
- Host species
Goat
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Conjugation
HRP polymer
- Applications
IHC-P, Ie
- Clonality
Polyclonal
Immunogen
- Full Length Protein corresponding to Rat Ig gamma-1 chain C region. The exact immunogen used to generate this antibody is proprietary information. Database link P20759
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Ie | Reactivity Reacts | Dilution info - | Notes This product is provided in a pre-diluted, ready-to-use format. Incubate 150 μl per slide for 1 hour. |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for IHC-P, Ie. Cited in 8 publications.
Key facts
- Description
Goat Anti-Rat IgG H&L (HRP polymer)
- Host species
Goat
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Conjugation
HRP polymer
- Applications
IHC-P, Ie
- Clonality
Polyclonal
- Pre-adsorbed
No
- Immunogen
- Full Length Protein corresponding to Rat Ig gamma-1 chain C region. The exact immunogen used to generate this antibody is proprietary information. Database link P20759
- Specificity
Based on IEP, ab214882 reacts with heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
Preservative: 0.1% Proclin 300
Constituents: 98% PBS, 1% BSA- Purification technique
Affinity purification
- Purification notes
ab214882 was affinity purified using solid phase Rat IgG. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Product promise
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10 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
C57BL/6 mouse spleen stained with 1 μg/ml of rat anti-mouse CD45R/B220 detected using ab214882 Goat Anti-Rat IgG H&L (HRP polymer).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CCXCR1/XCR1 with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Negative control: No staining on human kidney.
The section was incubated with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CCXCR1/XCR1 with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Positive staining on human Hodgkin's lymphoma.
The section was incubated with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCXCR1/XCR1 with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Positive staining on human tonisl.
The section was incubated with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CCXCR1/XCR1 with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Negative control: No staining on human skeletal muscle.
The section was incubated with Anti-CCXCR1/XCR1 antibody [CIA207A] ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Immunohistochemistry analysis of paraffin-embedded mouse lymph node tissue section labelling CSF-1-R with Anti-CSF-1-R antibody [YEYE311] ab271294 at 1/200 dilution. The section was incubated with Anti-CSF-1-R antibody [YEYE311] ab271294 at +4°C overnight. Goat Anti-Rat IgG H&L (HRP polymer) (ab214882) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Positive staining on the macrophages in mouse lymph node. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
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