Goat Anti-Rat IgG H&L (HRP) preadsorbed

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (AB7097)

This blot was produced using a 4-20% Criterion TGX gel in 1X SDS buffer. The gel was run at 180V for 40 minutes before being transferred onto a Nitrocellulose membrane at 25V for 7 minutes using the Trans-Blot turbo transfer system. The membrane was then blocked for an hour using 3% milk before being incubated with ab211293 overnight at 4°C at 1μg/ml. Antibody binding was detected using a Goat anti-Rat antibody conjugated to HRP, and visualised using Bio-Rad Clarity Western ECL substrate.

All lanes:

Western blot - Anti-PAX5 antibody [1H9] (<a href='/en-us/products/primary-antibodies/pax5-antibody-1h9-ab211293'>ab211293</a>) at 1 µg/mL

Lane 1:

Human tonsil whole tissue lysate at 20 µg

Lane 2:

Ramos whole cell lysate at 20 µg

Lane 3:

Daudi whole cell lysate at 20 µg

Lane 4:

Mouse spleen whole tissue lysate at 20 µg

Lane 5:

Rat spleen whole tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

Predicted band size: 42 kDa

Observed band size: 45 kDa

true

Exposure time: 10s

• WB

Supplier Data

Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (AB7097)

Both the heavy and light chains of rat IgG are detected.

All lanes:

Rat IgG, 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097) at 1/1000 dilution

false