Goat Anti-Rat IgG H&L (HRP) preadsorbed

2 product images

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

  Both the heavy and light chains of rat IgG are detected.

  All lanes: Rat IgG, 50 ng

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097) at 1/1000 dilution

• 

  Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

  This blot was produced using a 4-20% Criterion TGX gel in 1X SDS buffer. The gel was run at 180V for 40 minutes before being transferred onto a Nitrocellulose membrane at 25V for 7 minutes using the Trans-Blot turbo transfer system. The membrane was then blocked for an hour using 3% milk before being incubated with Anti-PAX5 antibody [1H9] ab211293 overnight at 4°C at 1μg/ml. Antibody binding was detected using a Goat anti-Rat antibody conjugated to HRP, and visualised using Bio-Rad Clarity Western ECL substrate.

  All lanes: Western blot - Anti-PAX5 antibody [1H9] (Anti-PAX5 antibody [1H9] ab211293) at 1 µg/mL

  Lane 1: Human tonsil whole tissue lysate at 20 µg

  Lane 2: Ramos whole cell lysate at 20 µg

  Lane 3: Daudi whole cell lysate at 20 µg

  Lane 4: Mouse spleen whole tissue lysate at 20 µg

  Lane 5: Rat spleen whole tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 42 kDa

  Observed band size: 45 kDa

  Exposure time: 10s