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Suitable for IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 22 publications.
Alternative names=Ig gamma-1 chain C region
- Host species
Goat
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Horse, Cow, Human
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Applications
IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF
- Clonality
Polyclonal
- Format
F(ab')2
- Immunogen
Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link: P20759
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes Use 1 x 106 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. |
Application IM | Reactivity Reacts | Dilution info 1/50 - 1/200 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes - |
Recommended products
Suitable for IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 22 publications.
Alternative names=Ig gamma-1 chain C region
Key facts
- Description
Goat anti-Rat IgG H&L (PE) Pre-Adsorbed
- Host species
Goat
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Horse, Cow, Human
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Applications
IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF
- Clonality
Polyclonal
- Format
F(ab')2
- Pre-adsorbed
Yes
- Immunogen
Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link: P20759
- Specificity
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Purification technique
Affinity purification Immunogen
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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