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Suitable for IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 22 publications.

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Publications

Key facts

Host species
Goat
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Sheep, Rabbit, Horse, Cow, Human
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Applications
IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF
Clonality
Polyclonal
Format
F(ab')2

Immunogen

  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759

Reactivity data

Application
IHC-Fr
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Expected
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Expected
Dilution info
1/100 - 1/250
Notes

Use 1 x 106 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.

Application
IM
Reactivity
Expected
Dilution info
1/50 - 1/200
Notes

-

Application
ICC/IF
Reactivity
Expected
Dilution info
1/100 - 1/250
Notes

-

Target data

Alternative names

Ig gamma-1 chain C region

Recommended products

Suitable for IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 22 publications.

Key facts

Description
Goat anti-Rat IgG H&L (PE) Pre-Adsorbed
Host species
Goat
Target species
Rat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Sheep, Rabbit, Horse, Cow, Human
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Applications
IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF
Clonality
Polyclonal
Format
F(ab')2
Pre-adsorbed
Yes
Immunogen
  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759
Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Product promise

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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