Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 4 publications.
Images
Publications
Filter by
- Nature communications 14:23532023HAPLN1 potentiates peritoneal metastasis in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLena Wiedmann,Francesca De Angelis Rigotti,Nuria Vaquero-Siguero,Elisa Donato,Elisa Espinet,Iris Moll,Elisenda Alsina-Sanchis,Hanibal Bohnenberger,Elena Fernandez-Florido,Ronja Mülfarth,Margherita Vacca,Jennifer Gerwing,Lena-Christin Conradi,Philipp Ströbel,Andreas Trumpp,Carolin Mogler,Andreas Fischer,Juan Rodriguez-VitaPubMed 37095087
- PloS one 18:e02840822023Corneal injury is associated with stromal and vascular alterations within cranial dura mater.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga V Glinskii,Vladislav V Glinsky,Leike Xie,Filiz Bunyak,Vladimir V Glinskii,Sunilima Sinha,Suneel Gupta,Renato V Iozzo,Rajiv R MohanPubMed 37079653
- Molecular cancer therapeutics 21:616-6242022Microneedle-mediated Intratumoral Delivery of Anti-CTLA-4 Promotes cDC1-dependent Eradication of Oral Squamous Cell Carcinoma with Limited irAEs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMara Gilardi,Robert Saddawi-Konefka,Victoria H Wu,Miguel Angel Lopez-Ramirez,Zhiyong Wang,Fernando Soto,Dana J Ramms,Marco Proietto,Zbigniew Mikulski,Haruka Miki,Andrew Sharabi,Daniel Kupor,Ricardo Rueda,Daniel P Hollern,Joseph Wang,J Silvio GutkindPubMed 35086958
- JCI insight 5:2020Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhanli Xie et. al.PubMed 32641582
Key facts
- Host species
- Goat
- Target species
- Syrian hamster
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 4 publications.
Key facts
- Description
- Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 647)
- Host species
- Goat
- Target species
- Syrian hamster
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 647) (ab180117)
IHC image of Podoplanin / gp36 staining in a section of formalin-fixed paraffin-embedded Mouse normal lung. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Anti-Podoplanin antibody [RTD4E10] - BSA and Azide free ab11936 at 1/100 dilution (Syrian hamster monoclonal [RTD4E10] to Podoplanin / gp36). The secondary antibody (shown in red) was ab180117 at 1/500 dilution (Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 647) and counterstained using Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195883, Rat monoclonal [YOL1/34] to Tubulin- Microtubule Marker (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Alexa Fluor® - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 647) (ab180117)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com