Horseradish peroxidase (HRP) is a rapid and stable enzyme commonly used as a detection reagent in immunoassays such as western blot, immunohistochemistry (IHC), and ELISA. The high turnover rate of HRP enables secondary antibodies conjugated to this enzyme to generate strong signals in short time spans.

HRP substrates can fall into different categories, including chromogenic (eg DAB, TMB, OPD), fluorogenic (eg ADHP), and chemiluminescent (eg ECL) depending on whether they produce a colored, fluorometric, or luminescent derivative.

We offer HRP secondary antibodies against diverse species and their isotypes, as well as in fragment and pre-adsorbed forms.

 
Western blotting secondary antibodies

These antibodies provide strong signal amplification and high resolution in western blot applications, making them ideal for detecting low-abundance proteins.

HRP-conjugated IHC secondary antibodies

Optimized for immunohistochemistry, these antibodies produce sharp, clear staining and enhance the visualization of target antigens in tissue sections.

Secondary antibodies for ELISA

These antibodies are designed to enhance ELISA assays with their high signal-to-noise ratio, ensuring accurate and reproducible quantification of antigens.

Horseradish peroxidase (HRP) conjugated secondary antibodies are an essential tool in the detection and quantification of target proteins in various immunoassays. By pairing with primary antibodies specific to an antigen of interest, HRP-conjugated secondary antibodies facilitate a cascade of signal amplification, leading to enhanced visibility of the targeted antigen. This is crucial in applications like western blot, immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA), where detecting minute amounts of a protein can be pivotal.

Our range of HRP-conjugated secondary antibodies is meticulously engineered to offer superior performance. Each antibody is conjugated to an optimal number of HRP enzymes to ensure high sensitivity, allowing you to detect even low-abundance targets with high clarity and minimal background noise. The robust nature of these antibodies allows for flexible use across a wide range of dilutions, making them cost-effective and adaptable to different experimental conditions. They also come in a ready-to-use liquid format that is stable and easy to store, adding convenience to the research workflow.

To address the challenge of cross-species reactivity, which can lead to non-specific binding and high background, we offer pre-adsorbed HRP-conjugated secondary antibodies. This specificity is particularly valuable in complex samples, enhancing the reliability of your results.