AB157532

Mouse Anti-goat IgG for IP (HRP)

VeriBlot
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(7 Publications)

Suitable for WB. Ideal for western blot. Cited in 7 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Mouse

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

applications

Clonality

Monoclonal

Clone number

eB270

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The dilution will depend on the sensitivity of the HRP substrate. The recommended dilution is 1 µl in 1 ml milk. Based on the recommended dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples.</p>" } } }

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Product details

Number of blots:
At least 20 blots (based on a 1 μl/ml dilution in 5 ml milk).

Important protocol notes:
1. The anti-goat VeriBlot for IP secondary antibody (HRP) detects goat IgG antibodies.
2. The anti-goat VeriBlot for IP secondary antibody (HRP) preferentially detects the non-reduced form of goat IgG over the reduced, SDS-denatured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.

Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips

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Properties and storage information

Form

Liquid

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.16% Sodium phosphate, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:4596-4607 PubMed33733587

2021

CDX2 enhances natural killer cell-mediated immunotherapy against head and neck squamous cell carcinoma through up-regulating CXCL14.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Wang,Shanji Nan,Ying Wang,Chengbi Xu

Molecular medicine reports 18:4239-4246 PubMed30221720

2018

Long non‑coding RNA ferritin heavy polypeptide 1 pseudogene 3 controls glioma cell proliferation and apoptosis via regulation of the microRNA‑224‑5p/tumor protein D52 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Zhang,Ying Li,Jing Wang,Ping Lei

Cellular physiology and biochemistry : internation 46:873-889 PubMed29669318

2018

NeuroD Expression in Podocytes and Interrelationships with Nephrin at Both Nuclear and Cytoplasmic Sites.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Armelloni,Masami Ikehata,Deborah Mattinzoli,Min Li,Carlo Maria Alfieri,Mariapia Rastaldi,Piergiorgio Messa

International journal of oncology 52:945-954 PubMed29393396

2018

PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Peng,Qiang Li,Jia-Yuan Sun,Ying Luo,Ming Chen,Yong Bao

PloS one 12:e0185289 PubMed28957379

2017

Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels.

Applications

Unspecified application

Species

Unspecified reactive species

Lizbeth Grimaldo,Alejandro Sandoval,Edgar Garza-López,Ricardo Felix

Molecular medicine reports 14:1631-5 PubMed27314868

2016

microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway.

Applications

Species

Unspecified reactive species

Gao Feng,Zhang Yan,Chuanchuan Li,Yuemei Hou

Molecular medicine reports 12:4598-4603 PubMed26126698

2015

Chitosan prevents adhesion during rabbit flexor tendon repair via the sirtuin 1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Chen,Hui Lu,Hu Yang

View all publications

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Product promise

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Mouse Anti-goat IgG for IP (HRP)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Clone number

Isotype

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (7)

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