Suitable for Dot, WB, ICC, IHC-Fr, ELISA. Ideal for western blot. Cited in 13 publications.
Mouse
Human
IgG4
Fc region
HRP
Dot, WB, ICC, IHC-Fr, ELISA
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes for Dot and slot immunoblotting. |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/1000 - 1/2000 | Notes - |
Select an associated product type
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
Suitable for Dot, WB, ICC, IHC-Fr, ELISA. Ideal for western blot. Cited in 13 publications.
Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)
Mouse
Human
IgG4
Fc region
HRP
Dot, WB, ICC, IHC-Fr, ELISA
Monoclonal
HP6023
No
IgG3
kappa
Liquid
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Affinity purification Protein A
Blue Ice
+4°C
+4°C
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ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99817.
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