AB99817

Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)

Name: Mouse Anti-Human IgG4 Fc (HRP) secondary antibody (ab99817) | Abcam
Brand: Abcam Limited
SKU: AB99817
Price: 330 USD
Availability: InStock

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(15 Publications)

Mouse Anti-Human IgG4 pFc’ (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ELISA, ICC and more.

- Cited in over 10 publications

View Alternative Names

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

1 Images

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817)

ELISA

Supplier Data

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817)

ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99817.

Key facts

Host species

Mouse

Target species

Human

Target isotype

IgG4

Target specificity

Fc region

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

ELISA, WB, Dot, IHC-Fr, ICC

applications

Clonality

Monoclonal

Clone number

HP6023

Isotype

IgG3

Light chain type

kappa

Immunogen

Full Length Protein corresponding to Human IGHG4. The exact immunogen used to generate this antibody is proprietary information.

P01861

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"for Dot and slot immunoblotting." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/2000", "notes":"" } } }

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Product details

This antibody reacts with the pFc' region of Human IgG4

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.

See full target information IGHG4

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

British journal of haematology : PubMed40386886

2025

Anti-HSP90α IgG may protect against antiphospholipid syndrome in systemic lupus erythematosus by reducing platelet activation.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Barguil Macêdo,Andreas Jönsen,Anders A Bengtsson,Iva Gunnarsson,Elisabet Svenungsson,Christian Lood

Diabetes, obesity & metabolism 26:1395-1406 PubMed38287130

2024

Safety, tolerability, pharmacokinetic, pharmacodynamic and immunogenicity profiles of Exendin-4-IgG4-Fc in healthy subjects: A phase 1, single-centre, randomized, double-blind, dose escalation study.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Ling Chen,Yang Liu,Xue-Feng Gao,Kai-Qi Wu,Yun-Kai Yang,Yong Chen,Cong-Gao Peng,Ting-Han Jin,Yu-Bao Huang,Yao-Wen Zhang,Jing Su,Qi Jiang,Tong Guo,Jie Zhao,Xiang-Nan Peng,Jing-Yu Peng,Si-Xiu Li,Yong-Li Sun,Hong-Mei Zhang,Yan-Li Fu,Dan Luo,Yaru Ma,Zhen-Wei Shen,Yun-Tao Zhang,Zhang-Fei Shou

Nature immunology 24:869-883 PubMed37081150

2023

Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jesus Corria-Osorio,Santiago J Carmona,Evangelos Stefanidis,Massimo Andreatta,Yaquelin Ortiz-Miranda,Tania Muller,Ioanna A Rota,Isaac Crespo,Bili Seijo,Wilson Castro,Cristina Jimenez-Luna,Leonardo Scarpellino,Catherine Ronet,Aodrenn Spill,Evripidis Lanitis,Pedro Romero,Sanjiv A Luther,Melita Irving,George Coukos

PloS one 18:e0279389 PubMed36763580

2023

Increased number of T cells and exacerbated inflammatory pathophysiology in a human IgG4 knock-in MRL/lpr mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshie Gon,Tsugumitsu Kandou,Tatsuaki Tsuruyama,Takeshi Iwasaki,Koji Kitagori,Kosaku Murakami,Ran Nakashima,Shuji Akizuki,Akio Morinobu,Masaki Hikida,Tsuneyo Mimori,Hajime Yoshifuji

Microbial cell factories 20:115 PubMed34107983

2021

Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Xu,Mingyang Shi,Changsheng Shao,Hao Li,Jing Wu,Yin Yu,Fang Fang,Yugang Guo,Weihua Xiao

Viruses 12: PubMed33138336

2020

Association of Genetic Polymorphisms in , , , and with the Clinical Forms of Dengue in Patients from Veracruz, Mexico.

Applications

Unspecified application

Species

Unspecified reactive species

Araceli Posadas-Mondragón,José Leopoldo Aguilar-Faisal,Gerardo Zuñiga,Jonathan Javier Magaña,José Angel Santiago-Cruz,Edith Guillén-Salomón,Verónica Alcántara-Farfán,María Luisa Arellano-Flores,Juan Santiago Salas-Benito,Rocío M Neri-Bazán,Lucero Luna-Rojas,Amanda Marineth Avila-Trejo,Adolfo Chávez-Negrete

Allergy 75:1835-1836 PubMed32652627

2020

Detection of peanut allergens in serum: circumventing the inhibitory effect of immunoglobulins.

Applications

Unspecified application

Species

Unspecified reactive species

Stef J Koppelman,Michel Witteveen,Lonneke JanssenDuijghuijsen,Joseph L Baumert,Renger F Witkamp,Klaske van Norren

Arthritis & rheumatology (Hoboken, N.J.) 72:687-693 PubMed31612628

2020

Disease Severity Linked to Increase in Autoantibody Diversity in IgG4-Related Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Liu,Cory A Perugino,Musie Ghebremichael,Zachary S Wallace,Sydney B Montesi,John H Stone,Shiv Pillai

Arteriosclerosis, thrombosis, and vascular biology 39:1884-1892 PubMed31315438

2019

Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report.

Applications

Unspecified application

Species

Unspecified reactive species

David Henson,Ayman Samman Tahhan,David Nardo,Arshed Ali Quyyumi,Vincent J Venditto

Molecular medicine reports 18:5191-5197 PubMed30272291

2018

Emodin suppresses angiogenesis and metastasis in anaplastic thyroid cancer by affecting TRAF6‑mediated pathways in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Hua Shi,Lin Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Clone number

Isotype

Light chain type

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (15)

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