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Suitable for Dot, WB, ICC, IHC-Fr, ELISA. Ideal for western blot. Cited in 13 publications.

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Images

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817), expandable thumbnail

Publications

Key facts

Host species

Mouse

Target species

Human

Target isotype

IgG4

Target specificity

Fc region

Conjugation

HRP

Applications

Dot, WB, ICC, IHC-Fr, ELISA

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link P01861

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Reactivity data

Application

Dot

Reactivity

Reacts

Dilution info

-

Notes

for Dot and slot immunoblotting.

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ICC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/1000 - 1/2000

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

Recommended products

Suitable for Dot, WB, ICC, IHC-Fr, ELISA. Ideal for western blot. Cited in 13 publications.

Key facts

Description

Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)

Host species

Mouse

Target species

Human

Target isotype

IgG4

Target specificity

Fc region

Conjugation

HRP

Applications

Dot, WB, ICC, IHC-Fr, ELISA

Clonality

Monoclonal

Clone number

HP6023

Pre-adsorbed

No

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link P01861
Isotype

IgG3

Light chain type

kappa

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS

Purification technique

Affinity purification Protein A

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Product promise

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1 product image

  • ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (ab99817), expandable thumbnail

    ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (ab99817)

    ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99817.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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