AB131367

Anti-mouse IgG for IP (Biotin)

VeriBlot
What is this?

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(1 Publication)

Suitable for WB. Cited in 1 publication.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Rat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

applications

Clonality

Monoclonal

Clone number

eB144

Isotype

IgG

Specificity

VeriBlot for IP secondary antibodies are anti-mouse immunoblotting reagents that enable the trouble-free detection of immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the (co-)immunoprecipitated protein without masking by the IgG heavy (50 kDa) and light chains (25 kDa). In general this interference tends to originate from secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself. VeriBlot for IP secondary antibodies only recognize native (non-reduced) antibodies and therefore the detection of heavy and light chains is highly minimized, if the immunoprecipitate is fully reduced.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.5 µg/mL", "notes":"<p>The dilution will depend on the sensitivity of the avidin/streptavidin HRP. The recommended is dilution is 0.5 µg/ml. Based on a 0.5 µg/ml dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples in combination with avidin/streptavidin HRP.</p>" } } }

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Product details

Number of blots:
At least 20 blots (based on a 0.5 μg/ml dilution in 5 ml milk).

Important protocol notes:
1. The anti-mouse VeriBlot for IP secondary antibody (Biotin) detects mouse IgG antibodies (subtypes IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse VeriBlot for IP secondary antibody (Biotin) preferentially detects the non-reduced form of mouse IgG (subtypes: IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-deantured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
5. Anti-mouse IgG VeriBlot for IP secondary antibody (Biotin) (ab131367) is the biotinylated form of the VeriBlot for IP anti-mouse HRP (ab131368). It can be used as a secondary antibody in combination with avidin/streptavidin HRP (ab59653/ab7403).

Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.09% Sodium azide Constituents: 0.88% Sodium chloride, 0.16% Sodium phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 12:e1002 PubMed36030524

2022

Translocation of gasdermin D induced mitochondrial injury and mitophagy mediated quality control in lipopolysaccharide related cardiomyocyte injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqing Yu,Zilong Xiao,Lichun Guan,Pei Bao,Yong Yu,Yixiu Liang,Minghui Li,Zhenzhen Huang,Xueying Chen,Ruizhen Chen,Yangang Su,Junbo Ge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-mouse IgG for IP (Biotin)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Clone number

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (1)

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise