Suitable for WB. Cited in 1 publication.
Rat
Mouse
IgG
Heavy & Light chains
Biotin
WB
Monoclonal
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 0.5 µg/mL | Notes The dilution will depend on the sensitivity of the avidin/streptavidin HRP. The recommended is dilution is 0.5 µg/ml. Based on a 0.5 µg/ml dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples in combination with avidin/streptavidin HRP. |
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Suitable for WB. Cited in 1 publication.
Anti-mouse IgG for IP (Biotin)
Rat
Mouse
IgG
Heavy & Light chains
Biotin
WB
Monoclonal
eB144
No
VeriBlot for IP secondary antibodies are anti-mouse immunoblotting reagents that enable the trouble-free detection of immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the (co-)immunoprecipitated protein without masking by the IgG heavy (50 kDa) and light chains (25 kDa). In general this interference tends to originate from secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself. VeriBlot for IP secondary antibodies only recognize native (non-reduced) antibodies and therefore the detection of heavy and light chains is highly minimized, if the immunoprecipitate is fully reduced.
IgG
Liquid
Preservative: 0.09% Sodium azide
Constituents: 0.88% Sodium chloride, 0.16% Sodium phosphate
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Number of blots:
At least 20 blots (based on a 0.5 μg/ml dilution in 5 ml milk).
Important protocol notes:
1. The anti-mouse VeriBlot for IP secondary antibody (Biotin) detects mouse IgG antibodies (subtypes IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse VeriBlot for IP secondary antibody (Biotin) preferentially detects the non-reduced form of mouse IgG (subtypes: IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-deantured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
5. Anti-mouse IgG VeriBlot for IP secondary antibody (Biotin) (ab131367) is the biotinylated form of the VeriBlot for IP anti-mouse HRP (Anti-mouse IgG for IP (HRP) ab131368). It can be used as a secondary antibody in combination with avidin/streptavidin HRP (Avidin Peroxidase Conjugate ab59653/Streptavidin (HRP) ab7403).
Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips
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