Anti-mouse IgG for IP (HRP)

28 product images

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  FCN2 has a high molecular weight (400 kDa) due to extensive disulfide bridge formation (PMID: 16595153)
  

  Negative control: Human brain (PMID: 8576206)

  A weak unidentifiable band was observed in brain tissue

  Blocking/diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-Ficolin 2 antibody [EPR23146-225] (Anti-Ficolin 2 antibody [EPR23146-225] ab267473) at 1/1000 dilution

  Lane 1: Human plasma at 20 µg

  Lane 2: Human liver tissue lysate at 20 µg

  Lane 3: Human brain tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 34 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Negative control: Mouse heart (PMID:15899979).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker ab254154).

  All lanes: Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker ab254154) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse heart tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 50 kDa

  Exposure time: 7s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  This data was developed using Anti-Myelin Basic Protein antibody [MBP2] ab11159 the same antibody clone in a different buffer formulation.
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Myelin Basic Protein antibody [MBP2] (Anti-Myelin Basic Protein antibody [MBP2] ab11159) at 1/1000 dilution

  All lanes: human brain tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/50000 dilution

  Predicted band size: 33 kDa

  Exposure time: 26s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking and dilution buffer: 5% NFDM/TBST.
  

  Exposure times.

  Lane 1: 125 seconds; Lanes 2-5: 26 seconds.

  The molecular weight observed is consistent with what has been described in the literature (PMID: 15236336).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (Anti-CASK antibody [K56A/50] ab252540).

  All lanes: Western blot - Anti-CASK antibody [K56A/50] (Anti-CASK antibody [K56A/50] ab252540) at 1/1000 dilution

  Lane 1: Human brain tissue lysate at 20 µg

  Lane 2: Mouse brain tissue lysate at 20 µg

  Lane 3: Mouse cerebellum lysate at 20 µg

  Lane 4: Rat brain tissue lysate at 20 µg

  Lane 5: Rat cerebellum lysate at 20 µg

  Secondary

  Lane 1: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/100000 dilution

  Lanes 2 - 5: Peroxidase-Conjugated Goat anti-Mouse IgG（H+L） at 1/100000 dilution

  Predicted band size: 105 kDa

  Observed band size: 120 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking and dilution buffer: 5% NFDM/TBST
  

  Exposure time:

  Lane 1: 15 seconds

  Lane 2: 7.75 seconds.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Elastin antibody [BA-4] ab9519).

  All lanes: Western blot - Anti-Elastin antibody [BA-4] - BSA and Azide free (Anti-Elastin antibody [BA-4] - BSA and Azide free ab269450) at 0.51 µg/mL

  Lane 1: Human artery tissue lysate at 20 µg

  Lane 2: Human skin tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 68 kDa

  Observed band size: 68 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24797388).

  This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (Anti-Properdin/PFC antibody [3A3E1] ab252531).

  All lanes: Western blot - Anti-Properdin/PFC antibody [3A3E1] (Anti-Properdin/PFC antibody [3A3E1] ab252531) at 0.454 µg/mL

  Lane 1: Human plasma lysate at 20 µg

  Lane 2: Human serum lysate at 20 µg

  Lane 3: Mouse plasma lysate at 20 µg

  Lane 4: Mouse serum lysate at 20 µg

  Lane 5: Rat plasma lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 51 kDa

  Observed band size: 55 kDa

  Exposure time: 1s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  IP sample preparation: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10⁷ Jurkat (Human T cell leukemia cell line from peripheral blood) cells/ml with 5 μg mouse anti-human Caspase 7.
  

  WB conditions: Precipitate from 1x10⁶ cells was subjected to electrophoresis, transferred to an PVDF membrane, and immunoblotted with an anti-Caspase 7 antibody.

  Detection:
  Lane 1: Detection with anti-mouse IgG for IP secondary antibody (HRP) (ab131368)
  Lane 2: Detection with an HRP-conjugated anti-mouse IgG H&L secondary antibody
  Lane 3: Lane 1 was re-immunoblotted using an HRP-conjugated anti-mouse IgG H&L secondary antibody. The heavy and light-chains can now be seen, confirming that although the immunoprecipitating heavy and light-chains are present, ab131368 detects only native antibody and not denatured heavy and light-chains.

  Please note the detection of the heavy and light-chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1.

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure times: Lane 1, 2: 3 seconds; Lane 3, 4: 7 seconds.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker ab254154).

  All lanes: Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker ab254154) at 1/1000 dilution

  Lane 1: Human hippocampus tissue lysate at 20 µg

  Lane 2: Human brain tissue lysate at 20 µg

  Lane 3: Rat hippocampus tissue lysate at 20 µg

  Lane 4: Rat brain tissue lysate at 20 µg

  Secondary

  Lanes 1 - 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

  Lanes 3 - 4: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 50 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This image was produced using the same antibody clone but in a different formulation Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] ab40, PBS, sodium azide, glycerol and BSA.

  All lanes: Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] ab40) at 1/1000 dilution

  All lanes: Human skin lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Observed band size: 58 kDa

  Exposure time: 114s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta II Tubulin antibody [7B9] ab28035).

  All lanes: Western blot - Anti-beta II Tubulin antibody [7B9] (Anti-beta II Tubulin antibody [7B9] ab28035) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Rat brain tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 50 kDa

  Exposure time: 1s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  Exposure times: Lane 1: 3 seconds; Lane 2-4: 1 second.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta III Tubulin antibody [AA10] ab231083).

  All lanes: Western blot - Anti-beta III Tubulin antibody [AA10] (Anti-beta III Tubulin antibody [AA10] ab231083) at 1/1000 dilution

  Lane 1: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg

  Lane 2: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 10 µg

  Lane 3: Mouse brain tissue lysate at 10 µg

  Lane 4: Rat brain tissue lysate at 10 µg

  Secondary

  Lane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

  Lanes 2 - 4: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 50 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  This data was developed using Anti-68kDa Neurofilament/NF-L antibody [NfL23] ab273442 the same antibody clone in a different buffer formulation.
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (Anti-68kDa Neurofilament/NF-L antibody [NfL23] ab273442) at 1/1000 dilution

  Lane 1: Mouse hippocampus tissue lysate at 20 µg

  Lane 2: Mouse liver tissue lysate at 20 µg

  Lane 3: Rat brain tissue lysate at 20 µg

  Lane 4: Rat hippocampus tissue lysate at 20 µg

  Lane 5: Rat liver tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 62 kDa

  Observed band size: 68 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  This data was developed using Anti-68kDa Neurofilament/NF-L antibody [Nfl21] ab273441 the same antibody clone in a different buffer formulation.
  

  Negative control: liver (PMID: 2120242).

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Exposure times: Lane 1-2: 10 seconds; Lane 3-5: 37 seconds.

  All lanes: Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (Anti-68kDa Neurofilament/NF-L antibody [Nfl21] ab273441) at 1/1000 dilution

  Lane 1: Mouse hippocampus tissue lysate at 20 µg

  Lane 2: Mouse liver tissue lysate at 20 µg

  Lane 3: Rat brain tissue lysate at 20 µg

  Lane 4: Rat hippocampus tissue lysate at 20 µg

  Lane 5: Rat liver tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 62 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  All lanes: Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] (Anti-Estrogen Receptor alpha antibody [CRET94D] ab241557) at 1/1000 dilution

  Lane 1: Human breast cancer tissue lysates at 20 µg

  Lane 2: Mouse breast tissue lysates at 20 µg

  Lane 3: Mouse ovary tissue lysates at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Predicted band size: 66 kDa

  Observed band size: 66 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking/Dilution buffer: 5% NFDM/TBST.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta II Tubulin antibody [7B9] ab28035).

  All lanes: Western blot - Anti-beta II Tubulin antibody [7B9] (Anti-beta II Tubulin antibody [7B9] ab28035) at 1/1000 dilution

  Lane 1: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

  Lane 2: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 50 kDa

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Exposure time: Lane 1: 32 seconds; Lane 2: 93 seconds

  All lanes: Western blot - Anti-Histone H3 antibody [1B1B2] (Anti-Histone H3 antibody [1B1B2] ab309551) at 1/1000 dilution

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Observed band size: 18 kDa

• 

  Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368)

  CP2c was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with Anti-CP2c antibody [14/LSF] ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CP2c antibody [14/LSF] ab308611 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
  Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
  Lane 2: Anti-CP2c antibody [14/LSF] ab308611 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
  Lane 3: Mouse monoclonal IgG (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of Anti-CP2c antibody [14/LSF] ab308611 in K-562 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 24 seconds

  All lanes: Immunoprecipitation - Anti-CP2c antibody [14/LSF] (Anti-CP2c antibody [14/LSF] ab308611) at 1/30 dilution

  All lanes: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Exposure time: 24s

• 

  Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368)

  Histone H3 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-Histone H3 antibody [1B1B2] ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3 antibody [1B1B2] ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  Lane 2: Anti-Histone H3 antibody [1B1B2] ab309551 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  Lane 3: Mouse IgG1 monoclonal isotype control (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of Anti-Histone H3 antibody [1B1B2] ab309551 in HeLa whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 32 seconds

  All lanes: Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] (Anti-Histone H3 antibody [1B1B2] ab309551) at 1/50 dilution

  All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Exposure time: 32s

• 

  Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368)

  Histone H3 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with Anti-Histone H3 antibody [1B1B2] ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3 antibody [1B1B2] ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
  Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
  Lane 2: Anti-Histone H3 antibody [1B1B2] ab309551 IP in C6 (rat glial tumor glial cell) whole cell lysate
  Lane 3: Mouse IgG1 monoclonal isotype control (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of Anti-Histone H3 antibody [1B1B2] ab309551 in C6 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 67 seconds

  All lanes: Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] (Anti-Histone H3 antibody [1B1B2] ab309551) at 1/50 dilution

  All lanes: C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Exposure time: 67s

• 

  Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368)

  Histone H3 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Histone H3 antibody [1B1B2] ab309551 at 1/50 dilution (2ug in 0.4mg lysates). Western blot was performed on the immunoprecipitate using Anti-Histone H3 antibody [1B1B2] ab309551 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
  Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 2: Anti-Histone H3 antibody [1B1B2] ab309551 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 3: Mouse IgG1 monoclonal isotype control (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of Anti-Histone H3 antibody [1B1B2] ab309551 in NIH/3T3 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 32 seconds

  All lanes: Immunoprecipitation - Anti-Histone H3 antibody [1B1B2] (Anti-Histone H3 antibody [1B1B2] ab309551) at 1/50 dilution

  All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Exposure time: 32s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  All lanes: Western blot - Anti-Biotin antibody [Hyb-8] (Anti-Biotin antibody [Hyb-8] ab201341) at 1/1000 dilution

  Lane 1: Human nerve lysate at 10 µg

  Lane 2: Human lower limb nerve lysate at 10 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Observed band size: 58 kDa

  Exposure time: 103s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  All lanes: Western blot - Anti-Biotin antibody [Hyb-8] (Anti-Biotin antibody [Hyb-8] ab201341) at 1/1000 dilution

  Lane 1: Mouse eyeball tissue lysate at 10 µg

  Lane 2: Rat eyeball tissue lysate at 10 µg

  Lane 3: Mouse C57 P20 retina at 10 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Observed band size: 36 kDa

  Exposure time: 3min

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-Human IgG antibody [IG266] (Anti-Human IgG antibody [IG266] ab200699) at 1/5000 dilution

  All lanes: Human serum at 10 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 36 kDa

  Observed band size: 50 kDa

  Exposure time: 8s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-Human IgG antibody [IG266] (Anti-Human IgG antibody [IG266] ab200699) at 1/1000 dilution

  Lane 1: Human spleen tissue lysate at 10 µg

  Lane 2: Human tonsil tissue lysate at 10 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

  Predicted band size: 36 kDa

  Observed band size: 50 kDa

  Exposure time: 6s

• 

  Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368)

  RAB10 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 ug with Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
  Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
  Lane 2: Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 IP in C6 (rat glial tumor glial cell) whole cell lysate
  Lane 3: Mouse IgG2a Isotype control (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 in C6 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 15 seconds.

  All lanes: Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296) at 1/30 dilution

  All lanes: C6 (rat glial tumor glial cell) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Exposure time: 15s

• 

  Western blot - Anti-mouse IgG for IP (HRP) (ab131368)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Exposure Time: Lane 1-3: 15 seconds, lane 4-5: 180 seconds.

  All lanes: Western blot - Anti-VAPB antibody [EPR27026-50] (Anti-VAPB antibody [EPR27026-50] ab315013) at 1/1000 dilution

  Lane 1: Human testis tissue lysate at 20 µg

  Lane 2: Human liver tissue lysate at 20 µg

  Lane 3: Mouse testis tissue lysate at 20 µg

  Lane 4: Mouse liver tissue lysate at 20 µg

  Lane 5: Mouse colon tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Observed band size: 27 kDa

• 

  Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368)

  RAB10 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
  Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 2: Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
  Lane 3: Mouse IgG2a Isotype control (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 in NIH/3T3 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 15 seconds.

  All lanes: Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296) at 1/30 dilution

  All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Exposure time: 15s

• 

  Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368)

  RAB10 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug with Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
  Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate
  Lane 2: Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate
  Lane 3: Mouse IgG2a Isotype control (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 in A549 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 15 seconds.

  All lanes: Immunoprecipitation - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296) at 1/30 dilution

  All lanes: A549 (human lung carcinoma epithelial cell) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (ab131368) at 1/5000 dilution

  Exposure time: 15s