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AB172333

Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 647)

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(5 Publications)

Suitable for IHC-Fr, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 5 publications.
2 Images
Flow Cytometry - Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 647) (AB172333)
  • Flow Cyt

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Flow Cytometry - Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 647) (AB172333)

Overlay histogram showing subclass specificity of ab172333. HeLa cells were fixed with 80% methanol (-20°C, 5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106) as indicated : ab16881 (Rat IgG1), ab90458 (Rat IgG2a), ab81461 (Rat IgG2b), ab50610 (Rat IgGc) for 30 min at 22°C. The secondary antibody (ab172333 - Mouse monoclonal (2A 8F4) Anti-Rat IgG2a, heavy chain - Alexa Fluor® 647) was used at 1/3000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.

Alexa Fluor® - Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 647) (AB172333)
  • Alexa Fluor®

Supplier Data

Alexa Fluor® - Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 647) (AB172333)

Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

  • 519 Alexa Fluor® 488

    Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 488)

Key facts

Host species

Mouse

Target species

Rat

Target isotype

IgG2a

Target specificity

Heavy chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

IHC-Fr, Flow Cyt

applications

Clonality

Monoclonal

Clone number

2A 8F4

Isotype

IgG1

Light chain type

kappa

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/3000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Rat IgG2a
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40912913

2025

Elucidating the role of human skeletal muscles in the pathogenesis of enterovirus D68 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Brigitta M Laksono,Atze J Bergsma,Alessandro Iuliano,Dominique Y Veldhoen,Stefan van Nieuwkoop,Marjan Boter,Lonneke Leijten,Lisa Bauer,Bas B Oude Munnink,Wwm Pim Pijnappel,Debby van Riel

Science advances 9:eabo2810 PubMed36857453

2023

Mechanisms of antigen-induced reversal of CNS inflammation in experimental demyelinating disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Lisen Lu,Kyle Binder,Jian Xiong,Lilin Ye,Yan H Cheng,Sonia Majri-Morrison,Wei Lu,Jae W Lee,Zhihong Zhang,Yu-Zhang Wu,Lixin Zheng,Michael J Lenardo

Nature communications 12:7296 PubMed34911975

2021

CD137 (4-1BB) costimulation of CD8 T cells is more potent when provided in cis than in trans with respect to CD3-TCR stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Itziar Otano,Arantza Azpilikueta,Javier Glez-Vaz,Maite Alvarez,José Medina-Echeverz,Ivan Cortés-Domínguez,Carlos Ortiz-de-Solorzano,Peter Ellmark,Sara Fritzell,Gabriela Hernandez-Hoyos,Michelle Hase Nelson,María Carmen Ochoa,Elixabet Bolaños,Doina Cuculescu,Patricia Jaúregui,Sandra Sanchez-Gregorio,Iñaki Etxeberria,María E Rodriguez-Ruiz,Miguel F Sanmamed,Álvaro Teijeira,Pedro Berraondo,Ignacio Melero

Nature immunology 18:321-333 PubMed28135254

2017

The IgM receptor FcμR limits tonic BCR signaling by regulating expression of the IgM BCR.

Applications

Unspecified application

Species

Unspecified reactive species

Trang T T Nguyen,Kathrin Kläsener,Christa Zürn,Patricia A Castillo,Ingrid Brust-Mascher,Denise M Imai,Charles L Bevins,Colin Reardon,Michael Reth,Nicole Baumgarth

eLife 5: PubMed27855783

2016

Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy.

Applications

IHC-P

Species

Unspecified reactive species

Shuhong Qi,Hui Li,Lisen Lu,Zhongyang Qi,Lei Liu,Lu Chen,Guanxin Shen,Ling Fu,Qingming Luo,Zhihong Zhang
View all publications
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