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AB172334

Mouse monoclonal [2B 10A8] Anti-Rat IgG2b heavy chain (Alexa Fluor® 488)

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(1 Publication)

Suitable for IHC-Fr, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 1 publication.
2 Images
Flow Cytometry - Mouse monoclonal [2B 10A8] Anti-Rat IgG2b heavy chain (Alexa Fluor® 488) (AB172334)
  • Flow Cyt

Unknown

Flow Cytometry - Mouse monoclonal [2B 10A8] Anti-Rat IgG2b heavy chain (Alexa Fluor® 488) (AB172334)

Overlay histogram showing subclass specificity of ab172334. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated : ab16881 (Rat IgG1), ab90458 (Rat IgG2a), ab81461 (Rat IgG2b), ab50610 (Rat IgGc) for 30 min at 22°C. The secondary antibody (ab172334 - Mouse monoclonal (2B 10A8) Anti-Rat IgG2b, heavy chain - Alexa Fluor® 488) was used at 1/3000 dilution for 30 min at 22°C. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Alexa Fluor® - Mouse monoclonal [2B 10A8] Anti-Rat IgG2b heavy chain (Alexa Fluor® 488) (AB172334)
  • Alexa Fluor®

Supplier Data

Alexa Fluor® - Mouse monoclonal [2B 10A8] Anti-Rat IgG2b heavy chain (Alexa Fluor® 488) (AB172334)

Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.

  • 665 Alexa Fluor® 647

    Mouse monoclonal [2B 10A8] Anti-Rat IgG2b heavy chain (Alexa Fluor® 647)

Key facts

Host species

Mouse

Target species

Rat

Target isotype

IgG2b

Target specificity

Heavy chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

Flow Cyt, IHC-Fr

applications

Clonality

Monoclonal

Clone number

2B 10A8

Isotype

IgG2b

Light chain type

kappa

Specificity

Reacts with y2b heavy chain (Fc) of rat IgG2b.

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Complementary Products

Secondary Antibodies

AB172332

Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 488)

Flow Cytometry - Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 488) (AB172332)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB97245

Goat Anti-Mouse IgG2a heavy chain (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (AB97245)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/3000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Rat IgG2b
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:7296 PubMed34911975

2021

CD137 (4-1BB) costimulation of CD8 T cells is more potent when provided in cis than in trans with respect to CD3-TCR stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Itziar Otano,Arantza Azpilikueta,Javier Glez-Vaz,Maite Alvarez,José Medina-Echeverz,Ivan Cortés-Domínguez,Carlos Ortiz-de-Solorzano,Peter Ellmark,Sara Fritzell,Gabriela Hernandez-Hoyos,Michelle Hase Nelson,María Carmen Ochoa,Elixabet Bolaños,Doina Cuculescu,Patricia Jaúregui,Sandra Sanchez-Gregorio,Iñaki Etxeberria,María E Rodriguez-Ruiz,Miguel F Sanmamed,Álvaro Teijeira,Pedro Berraondo,Ignacio Melero
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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